Objective To compare the effects of 2 dosage-forms (granular and decoction) of Arnebia euchroma (royle) Johnst (Arnebia EJ in short below) on medical abortion with that of mifepristone combined with misoprostol Met...Objective To compare the effects of 2 dosage-forms (granular and decoction) of Arnebia euchroma (royle) Johnst (Arnebia EJ in short below) on medical abortion with that of mifepristone combined with misoprostol Methods Totally 648 women, who had pregnancy of 38-45 d and were willing to terminate pregnancy with mifepristone and misoprostol, were randomly divided into 3 groups, each of which was respectively given granular of Arnebia EJ, placebo granular, or decoction of Arnebia EJ besides mifepristone and misoprostol. The abortion results, bleeding duration, menstruation recovery and side-effects were observed. Results Neither complete abortion rates nor average bleeding durations of the granular group and the decoction group were significantly different (P>0.05). The complete abortion rate and bleeding duration of the two groups were respectively higher and shorter than those of the placebo group (P<0.05). However, the menstruation recov- ery was not significantly different among the three groups (P>0.05). The decoction of Arnebia EJ caused significantly more nausea and vomiting than the other groups (P<0.05). Conclusion The granular form did not have the odor of Arnebia EJ, and caused much less nausea and vomiting compared with the decoction form. The granular and decoc- tion forms were equally effective in improving the results of medical abortion. There- fore it is necessary to conduct further studies on the granular form of Arnebia EJ.展开更多
AIM: To evaluate the therapeutic effect of hydroxynaphthoquinone mixture (HM) on dextran sulfate sodium (DSS)-induced colitis and explore the underlying mechanisms.
AIM:To evaluate the potential effectiveness of hydroxynaphthoquinone mixture(HM)in rats with 2,4,6-trinitrobenzene sulfonic acid(TNBS)-induced colitis.METHODS:Colitis was induced by intracolonic administration of TNBS...AIM:To evaluate the potential effectiveness of hydroxynaphthoquinone mixture(HM)in rats with 2,4,6-trinitrobenzene sulfonic acid(TNBS)-induced colitis.METHODS:Colitis was induced by intracolonic administration of TNBS(80 mg/kg,dissolved in 50%ethanol).Rats were treated daily for 7 d with HM(2.5,5,10 mg/kg)and mesalazine 100 mg/kg 24 h after TNBS instillation.Disease progression was monitored daily by observation of clinical signs and body weight change.At the end of the experiment,macroscopic and histopathologic lesions of rats were scored,and myeloperoxidase(MPO)activity was determined.We also determined inflammatory cytokine tumor necrosis factor(TNF)-αlevel by ELISA,Western blotting and immunochemistry to explore the potential mechanisms of HM.RESULTS:After intracolonic instillation of TNBS,animals developed colitis associated with soft stool,diarrhea and marked colonic destruction.Administration of HM significantly attenuated clinical and histopathologic severity of TNBS-induced colitis in a dose-dependent manner.It abrogated body weight loss,diarrhea and inflammation,decreased macroscopic damage score,and improved histological signs,with a significant reduction of inflammatory infiltration,ulcer size and the severity of goblet cell depletion(all P<0.05 vs TNBS alone group).HM could reduce MPO activity.In addition,it also decreased serum TNF-αlevel and down-regulated TNF-αexpression in colonic tissue.This reduction was statistically significant when the dose of HM was 10 mg/kg(P<0.05 vs TNBS alone group),and the effect was comparable to that of mesalazine and showed no apparent adverse effect.The underlying mechanism may be associated with TNF-αinhibition.CONCLUSION:These findings suggest that HM possesses favourable therapeutic action in TNBS-induced colitis,which provides direct pharmacological evidence for its clinical application.展开更多
Objectives:To investigate the diversity and community composition of endophytes in Sinopodophyllum hexandrum(Royle)Ying.Methods:S.hexandrum samples(n=15)were collected at five sites on the Tibetan Plateau.High quality...Objectives:To investigate the diversity and community composition of endophytes in Sinopodophyllum hexandrum(Royle)Ying.Methods:S.hexandrum samples(n=15)were collected at five sites on the Tibetan Plateau.High quality sequences from high-throughput sequencing were clustered into operational taxonomic units(OTUs)by Clustering and Classification Inference with U-Statistics,and the OTUs were further divided into different genera according to Ribosomal Database Project classification.Alpha and beta diversity analyses were conducted to explore community diversity and composition.Results:A total of 1221 OTUs were obtained,representing 133 genera(with 98 specific to genus).The QJ and XH sampling sites had the most(n=67)and fewest(n=36)genera,respectively.The dominant fungi at the DT,LD,QJ and XH sites were Ascomycota,while the dominant fungi at the BX site were Basidiomycota.Tetracladium,Exophiala and Pseudogymnoascus showed obvious host preference in all samples.The dominant fungi in the three provinces were Tetracladium,Penicillium,and Cantharellales,respectively.The Shannon-Weiner diversity indices of the 15 samples ranged from 0.48 to 3.10.Endophytic fungi were clustered into three branches based on geographical location.Conclusion:Our findings provide a research basis for the exploitation and utilization of national medicinal plants with abundant microbial resources.展开更多
文摘Objective To compare the effects of 2 dosage-forms (granular and decoction) of Arnebia euchroma (royle) Johnst (Arnebia EJ in short below) on medical abortion with that of mifepristone combined with misoprostol Methods Totally 648 women, who had pregnancy of 38-45 d and were willing to terminate pregnancy with mifepristone and misoprostol, were randomly divided into 3 groups, each of which was respectively given granular of Arnebia EJ, placebo granular, or decoction of Arnebia EJ besides mifepristone and misoprostol. The abortion results, bleeding duration, menstruation recovery and side-effects were observed. Results Neither complete abortion rates nor average bleeding durations of the granular group and the decoction group were significantly different (P>0.05). The complete abortion rate and bleeding duration of the two groups were respectively higher and shorter than those of the placebo group (P<0.05). However, the menstruation recov- ery was not significantly different among the three groups (P>0.05). The decoction of Arnebia EJ caused significantly more nausea and vomiting than the other groups (P<0.05). Conclusion The granular form did not have the odor of Arnebia EJ, and caused much less nausea and vomiting compared with the decoction form. The granular and decoc- tion forms were equally effective in improving the results of medical abortion. There- fore it is necessary to conduct further studies on the granular form of Arnebia EJ.
基金Supported by Programs for Science and Technology Development and Plan of Yantai,No.2013ZH086
文摘AIM: To evaluate the therapeutic effect of hydroxynaphthoquinone mixture (HM) on dextran sulfate sodium (DSS)-induced colitis and explore the underlying mechanisms.
基金Supported by National Program for Important New Drugs R and D,No.2011ZX9102-006-04Programs for Science and Technology Development and Plan of Yantai,No.2013ZH086
文摘AIM:To evaluate the potential effectiveness of hydroxynaphthoquinone mixture(HM)in rats with 2,4,6-trinitrobenzene sulfonic acid(TNBS)-induced colitis.METHODS:Colitis was induced by intracolonic administration of TNBS(80 mg/kg,dissolved in 50%ethanol).Rats were treated daily for 7 d with HM(2.5,5,10 mg/kg)and mesalazine 100 mg/kg 24 h after TNBS instillation.Disease progression was monitored daily by observation of clinical signs and body weight change.At the end of the experiment,macroscopic and histopathologic lesions of rats were scored,and myeloperoxidase(MPO)activity was determined.We also determined inflammatory cytokine tumor necrosis factor(TNF)-αlevel by ELISA,Western blotting and immunochemistry to explore the potential mechanisms of HM.RESULTS:After intracolonic instillation of TNBS,animals developed colitis associated with soft stool,diarrhea and marked colonic destruction.Administration of HM significantly attenuated clinical and histopathologic severity of TNBS-induced colitis in a dose-dependent manner.It abrogated body weight loss,diarrhea and inflammation,decreased macroscopic damage score,and improved histological signs,with a significant reduction of inflammatory infiltration,ulcer size and the severity of goblet cell depletion(all P<0.05 vs TNBS alone group).HM could reduce MPO activity.In addition,it also decreased serum TNF-αlevel and down-regulated TNF-αexpression in colonic tissue.This reduction was statistically significant when the dose of HM was 10 mg/kg(P<0.05 vs TNBS alone group),and the effect was comparable to that of mesalazine and showed no apparent adverse effect.The underlying mechanism may be associated with TNF-αinhibition.CONCLUSION:These findings suggest that HM possesses favourable therapeutic action in TNBS-induced colitis,which provides direct pharmacological evidence for its clinical application.
基金The Qinghai Province Key Research&Development and Transformation Plan Project in 2023(2023-NK-133)The Special Fund for Innovation Capacity Construction of Scientific Research Institutions in Guizhou Province(QIANKEHEFUQI(2021)2,QIANKEHEFUQI[2024]010)。
文摘Objectives:To investigate the diversity and community composition of endophytes in Sinopodophyllum hexandrum(Royle)Ying.Methods:S.hexandrum samples(n=15)were collected at five sites on the Tibetan Plateau.High quality sequences from high-throughput sequencing were clustered into operational taxonomic units(OTUs)by Clustering and Classification Inference with U-Statistics,and the OTUs were further divided into different genera according to Ribosomal Database Project classification.Alpha and beta diversity analyses were conducted to explore community diversity and composition.Results:A total of 1221 OTUs were obtained,representing 133 genera(with 98 specific to genus).The QJ and XH sampling sites had the most(n=67)and fewest(n=36)genera,respectively.The dominant fungi at the DT,LD,QJ and XH sites were Ascomycota,while the dominant fungi at the BX site were Basidiomycota.Tetracladium,Exophiala and Pseudogymnoascus showed obvious host preference in all samples.The dominant fungi in the three provinces were Tetracladium,Penicillium,and Cantharellales,respectively.The Shannon-Weiner diversity indices of the 15 samples ranged from 0.48 to 3.10.Endophytic fungi were clustered into three branches based on geographical location.Conclusion:Our findings provide a research basis for the exploitation and utilization of national medicinal plants with abundant microbial resources.
