为明确不同圆柱山羊草种群对甲基二磺隆的敏感性差异机制,本研究以采自我国4个省(市)的10个圆柱山羊草种群为研究对象,利用整株生物测定法评估其对甲基二磺隆的敏感性水平。结果显示,不同圆柱山羊草种群对甲基二磺隆的敏感性存在差异(GR...为明确不同圆柱山羊草种群对甲基二磺隆的敏感性差异机制,本研究以采自我国4个省(市)的10个圆柱山羊草种群为研究对象,利用整株生物测定法评估其对甲基二磺隆的敏感性水平。结果显示,不同圆柱山羊草种群对甲基二磺隆的敏感性存在差异(GR_(50)=3.84~10.87 g a.i.·hm^(-2)),其中HB-1种群对甲基二磺隆最敏感(GR_(50)=3.84 g a.i.·hm^(-2)),HB-2种群对甲基二磺隆的敏感性较低(GR_(50)=10.87 g a.i.·hm^(-2))。ALS基因比对发现,HB-1和HB-2种群ALS基因序列完全一致,在已知的与除草剂抗性相关的9个关键位点无任何氨基酸缺失、插入或突变。乙酰乳酸合成酶(ALS)离体活性测定结果显示,HB-1和HB-2种群ALS对甲基二磺隆的敏感性无明显差异,其I50值分别为0.174和0.166μmol·L^(-1)。ALS基因表达量测定结果显示,甲基二磺隆处理后的HB-2种群ALS基因表达量整体极显著高于HB-1;施药后7 d,HB-2种群ALS基因的绝对表达量和相对表达量分别是HB-1的8.62和10.28倍。以上结果表明,ALS基因转录水平不同是导致圆柱山羊草不同种群对甲基二磺隆敏感性差异的主要原因之一。本研究结果为圆柱山羊草的区域性防控提供了理论依据。展开更多
目的探讨微小RNA-6321(microRNA-6321,miR-6321)对大鼠心肌缺血再灌注损伤(myocardial ischemia-reperfusion injury,MIRI)的作用,并进一步分析其作用机制与N6-甲基腺苷甲基化之间的关系。方法随机从36只雄性SD大鼠中取12只,分为预实验...目的探讨微小RNA-6321(microRNA-6321,miR-6321)对大鼠心肌缺血再灌注损伤(myocardial ischemia-reperfusion injury,MIRI)的作用,并进一步分析其作用机制与N6-甲基腺苷甲基化之间的关系。方法随机从36只雄性SD大鼠中取12只,分为预实验假手术组和预实验模型组,每组6只,用于验证MIRI模型。预实验假手术组在左前降支下方穿线而不结扎,预实验模型组结扎左前降支30 min后解开结扎线再灌注2 h。随后,本研究将剩余24只大鼠随机分为4组:假手术组、模型组(MIRI组)、腺相关病毒阴性对照组(adeno-associated virus negative control group,AAV-NC组)以及miR-6321过表达干预组(AAV-miR-6321组),每组6只。AAV-NC组和AAV-miR-6321组在模型建立前两周经尾静脉单次注射AAV-NC和AAV-miR-6321。采用冠状动脉左前降支结扎法建立大鼠MIRI模型。假手术组在左前降支下方穿线而不结扎,MIRI组、AAV-NC组和AAV-miR-6321组均进行缺血30 min、再灌注120 min处理。采用苏木精-伊红染色检测分析心肌组织损伤程度,蛋白质免疫印迹法检测心肌组织中甲基转移酶样蛋白3(methyltransferase-like protein 3,METTL3)、烷基化修复同系物5(alkylation repair homolog 5,ALKBH5)及YTH结构域家族蛋白1(YTH domain family protein 1,YTHDF1)表达水平。结果与预实验假手术组比较,预实验模型组ALKBH5的表达显著下降,METTL3、YTHDF1的表达显著上调,差异有统计学意义(P<0.05)。与假手术组比较,MIRI组心肌损伤程度显著升高,miR-6321表达显著降低,METTL3和YTHDF1表达显著升高(0.96±0.06 vs 0.52±0.08,1.04±0.04 vs 0.47±0.04,P<0.05),ALKBH5表达显著降低(0.36±0.14 vs 0.89±0.09,P<0.05);与MIRI组比较,AAV-miR-6321组心肌损伤程度显著降低,miR-6321表达显著升高,METTL3和YTHDF1表达显著降低(0.58±0.05 vs 0.96±0.06,0.55±0.04 vs 1.04±0.04,P<0.05),ALKBH5表达显著升高(0.76±0.14 vs 0.36±0.14,P<0.05)。结论miR-6321可调控N6-甲基腺苷甲基化修饰从而减轻大鼠心肌缺血再灌注损伤。展开更多
N-methyl pyrrolidone,hydrochloric acid and thiourea dioxide were adopted as the raw material,and 4-methyl guanidine butyric acid was synthesized through two-step reaction.The optimum synthesis condition for the first ...N-methyl pyrrolidone,hydrochloric acid and thiourea dioxide were adopted as the raw material,and 4-methyl guanidine butyric acid was synthesized through two-step reaction.The optimum synthesis condition for the first step was as follows：n（N-methyl pyrrolidone）∶n（10% HCl）= 1∶2.0,reaction temperature 135 ℃,reaction time 5 h;at that moment,the yield of intermediate 4-methyl-amino butyric acid hydrochloride was 72.89%.The optimum synthesis condition for the second step was as follows：n（4-methyl-amino butyric acid hydrochloride）∶n（thiourea dioxide）= 1∶2.0,reaction temperature 25 ℃,reaction time 12 h,at that moment,the yield of target product was 82.68%.Structure characterization on the intermediates and the target products were carried out through Fourier transform infrared spectroscopy and elemental analysis.展开更多
[ Objective] The aim was to select the best additives of bensulfuron-methyl. [ Method ] Taking radish as the testing plant, the synergistic effect of five common additives including Span 60, methyl olelate, silicone o...[ Objective] The aim was to select the best additives of bensulfuron-methyl. [ Method ] Taking radish as the testing plant, the synergistic effect of five common additives including Span 60, methyl olelate, silicone oil I, Tween 20 and detergent on the herbicidal activities of bensulfuron-methyl were tested in the la- borotory. [ Results ] The effects of the mixture of various additives and bensulfuran-methyl on relative stem control effect of radish, relative inhibition rate against stem length, relative inhibition rote against abeveground fresh weight, relative inhibition rote aginst root length and root fresh weight were studied, and the results showed that Span 60 and methyl olelate had stronger synergistic effect on the herbicidal activities of bensulfuron-methyl, and the synergistic effect of detergent was the weakest. [ Conclusion ] Span 60 and methyl olelate could be used as the synergistic agents of bensulfuron-methyl, and the study could provide references for se- lecting the additives of bensulfuron-methyl.展开更多
文摘为明确不同圆柱山羊草种群对甲基二磺隆的敏感性差异机制,本研究以采自我国4个省(市)的10个圆柱山羊草种群为研究对象,利用整株生物测定法评估其对甲基二磺隆的敏感性水平。结果显示,不同圆柱山羊草种群对甲基二磺隆的敏感性存在差异(GR_(50)=3.84~10.87 g a.i.·hm^(-2)),其中HB-1种群对甲基二磺隆最敏感(GR_(50)=3.84 g a.i.·hm^(-2)),HB-2种群对甲基二磺隆的敏感性较低(GR_(50)=10.87 g a.i.·hm^(-2))。ALS基因比对发现,HB-1和HB-2种群ALS基因序列完全一致,在已知的与除草剂抗性相关的9个关键位点无任何氨基酸缺失、插入或突变。乙酰乳酸合成酶(ALS)离体活性测定结果显示,HB-1和HB-2种群ALS对甲基二磺隆的敏感性无明显差异,其I50值分别为0.174和0.166μmol·L^(-1)。ALS基因表达量测定结果显示,甲基二磺隆处理后的HB-2种群ALS基因表达量整体极显著高于HB-1;施药后7 d,HB-2种群ALS基因的绝对表达量和相对表达量分别是HB-1的8.62和10.28倍。以上结果表明,ALS基因转录水平不同是导致圆柱山羊草不同种群对甲基二磺隆敏感性差异的主要原因之一。本研究结果为圆柱山羊草的区域性防控提供了理论依据。
文摘目的探讨微小RNA-6321(microRNA-6321,miR-6321)对大鼠心肌缺血再灌注损伤(myocardial ischemia-reperfusion injury,MIRI)的作用,并进一步分析其作用机制与N6-甲基腺苷甲基化之间的关系。方法随机从36只雄性SD大鼠中取12只,分为预实验假手术组和预实验模型组,每组6只,用于验证MIRI模型。预实验假手术组在左前降支下方穿线而不结扎,预实验模型组结扎左前降支30 min后解开结扎线再灌注2 h。随后,本研究将剩余24只大鼠随机分为4组:假手术组、模型组(MIRI组)、腺相关病毒阴性对照组(adeno-associated virus negative control group,AAV-NC组)以及miR-6321过表达干预组(AAV-miR-6321组),每组6只。AAV-NC组和AAV-miR-6321组在模型建立前两周经尾静脉单次注射AAV-NC和AAV-miR-6321。采用冠状动脉左前降支结扎法建立大鼠MIRI模型。假手术组在左前降支下方穿线而不结扎,MIRI组、AAV-NC组和AAV-miR-6321组均进行缺血30 min、再灌注120 min处理。采用苏木精-伊红染色检测分析心肌组织损伤程度,蛋白质免疫印迹法检测心肌组织中甲基转移酶样蛋白3(methyltransferase-like protein 3,METTL3)、烷基化修复同系物5(alkylation repair homolog 5,ALKBH5)及YTH结构域家族蛋白1(YTH domain family protein 1,YTHDF1)表达水平。结果与预实验假手术组比较,预实验模型组ALKBH5的表达显著下降,METTL3、YTHDF1的表达显著上调,差异有统计学意义(P<0.05)。与假手术组比较,MIRI组心肌损伤程度显著升高,miR-6321表达显著降低,METTL3和YTHDF1表达显著升高(0.96±0.06 vs 0.52±0.08,1.04±0.04 vs 0.47±0.04,P<0.05),ALKBH5表达显著降低(0.36±0.14 vs 0.89±0.09,P<0.05);与MIRI组比较,AAV-miR-6321组心肌损伤程度显著降低,miR-6321表达显著升高,METTL3和YTHDF1表达显著降低(0.58±0.05 vs 0.96±0.06,0.55±0.04 vs 1.04±0.04,P<0.05),ALKBH5表达显著升高(0.76±0.14 vs 0.36±0.14,P<0.05)。结论miR-6321可调控N6-甲基腺苷甲基化修饰从而减轻大鼠心肌缺血再灌注损伤。
文摘N-methyl pyrrolidone,hydrochloric acid and thiourea dioxide were adopted as the raw material,and 4-methyl guanidine butyric acid was synthesized through two-step reaction.The optimum synthesis condition for the first step was as follows：n（N-methyl pyrrolidone）∶n（10% HCl）= 1∶2.0,reaction temperature 135 ℃,reaction time 5 h;at that moment,the yield of intermediate 4-methyl-amino butyric acid hydrochloride was 72.89%.The optimum synthesis condition for the second step was as follows：n（4-methyl-amino butyric acid hydrochloride）∶n（thiourea dioxide）= 1∶2.0,reaction temperature 25 ℃,reaction time 12 h,at that moment,the yield of target product was 82.68%.Structure characterization on the intermediates and the target products were carried out through Fourier transform infrared spectroscopy and elemental analysis.
基金Supported by Doctoral Science Foundation in Yangtze University~~
文摘[ Objective] The aim was to select the best additives of bensulfuron-methyl. [ Method ] Taking radish as the testing plant, the synergistic effect of five common additives including Span 60, methyl olelate, silicone oil I, Tween 20 and detergent on the herbicidal activities of bensulfuron-methyl were tested in the la- borotory. [ Results ] The effects of the mixture of various additives and bensulfuran-methyl on relative stem control effect of radish, relative inhibition rate against stem length, relative inhibition rote against abeveground fresh weight, relative inhibition rote aginst root length and root fresh weight were studied, and the results showed that Span 60 and methyl olelate had stronger synergistic effect on the herbicidal activities of bensulfuron-methyl, and the synergistic effect of detergent was the weakest. [ Conclusion ] Span 60 and methyl olelate could be used as the synergistic agents of bensulfuron-methyl, and the study could provide references for se- lecting the additives of bensulfuron-methyl.
