This review focuses on the occurrence and interactions of engineered nanoparticles(ENPs)and brominated flame retardants(BFRs)such as polybrominated diphenyl ethers(PBDEs)in water systems and the generation of highly c...This review focuses on the occurrence and interactions of engineered nanoparticles(ENPs)and brominated flame retardants(BFRs)such as polybrominated diphenyl ethers(PBDEs)in water systems and the generation of highly complex compounds in the environment.The release of ENPs and BFRs(e.g.PBDEs)to aquatic environments during their usage and disposal are summarised together with their key interaction mechanisms.The major interaction mechanisms including electrostatic,van derWaals,hydrophobic,molecular bridging and steric,hydrogen andπ-bonding,cation bridging and ligand exchange were identified.The presence of ENPs could influence the fate and behaviour of PBDEs through the interactions as well as induced reactions under certain conditions which increases the formation of complex compounds.The interaction leads to alteration of behaviour for PBDEs and their toxic effects to ecological receptors.The intermingled compound(ENPs-BFRs)would show different behaviour from the parental ENPs or BFRs,which are currently lack of investigation.This review provided insights on the interactions of ENPs and BFRs in artificial,environmental water systems and wastewater treatment plants(WWTPs),which are important for a comprehensive risk assessment.展开更多
Effective risk assessment and control of environmental antibiotic resistance depend on comprehensive information about antibiotic resistance genes(ARGs)and their microbial hosts.Advances in sequencing technologies and...Effective risk assessment and control of environmental antibiotic resistance depend on comprehensive information about antibiotic resistance genes(ARGs)and their microbial hosts.Advances in sequencing technologies and bioinformatics have enabled the identification of ARG hosts using metagenomeassembled contigs and genomes.However,these approaches often suffer from information loss and require extensive computational resources.Here we introduce a bioinformatic strategy that identifies ARG hosts by prescreening ARG-like reads(ALRs)directly from total metagenomic datasets.This ALRbased method offers several advantages:(1)it enables the detection of low-abundance ARG hosts with higher accuracy in complex environments;(2)it establishes a direct relationship between the abundance of ARGs and their hosts;and(3)it reduces computation time by approximately 44e96%compared to strategies relying on assembled contigs and genomes.We applied our ALR-based strategy alongside two traditional methods to investigate a typical human-impacted environment.The results were consistent across all methods,revealing that ARGs are predominantly carried by Gammaproteobacteria and Bacilli,and their distribution patterns may indicate the impact of wastewater discharge on coastal resistome.Our strategy provides rapid and accurate identification of antibiotic-resistant bacteria,offering valuable insights for the high-throughput surveillance of environmental antibiotic resistance.This study further expands our knowledge of ARG-related risk management in future.展开更多
Class 1 integrons facilitate horizontal gene transfer,significantly influencing antibiotic resistance gene(ARG)dissemination within microbial communities.Wastewater treatment plants(WWTPs)are critical reservoirs of AR...Class 1 integrons facilitate horizontal gene transfer,significantly influencing antibiotic resistance gene(ARG)dissemination within microbial communities.Wastewater treatment plants(WWTPs)are critical reservoirs of ARGs and integrons,yet the integron-mediated dynamics of ARG transfer across different WWTP types remain poorly understood.Here we show distinct ARG profiles associated with class 1 integrons in municipal and industrial WWTPs using a novel approach combining nested-like highthroughput q PCR and Pac Bio sequencing.Although industrial WWTPs contained higher absolute integron abundances,their relative ARG content was lower(1.27×10^(7)–9.59×10^(7) copies per ng integron)compared to municipal WWTPs(3.72×10^(7)–1.98×10^(8) copies per ng integron).Of the 132,084 coding sequences detected from integrons,56.8%encoded antibiotic resistance,with industrial plants showing lower ARG proportions,reduced ARG array diversity,and greater incorporation of non-ARG sequences.These findings suggest industrial WWTP integrons integrate a broader array of exogenous genes,reflecting adaptation to complex wastewater compositions.This work enhances our understanding of integron-driven ARG dynamics in wastewater and offers a robust strategy for environmental integron analysis.展开更多
Antimicrobial resistance(AMR)has emerged as a significant challenge in human health.Wastewater treatment plants(WWTPs),acting as a link between human activities and the environment,create ideal conditions for the sele...Antimicrobial resistance(AMR)has emerged as a significant challenge in human health.Wastewater treatment plants(WWTPs),acting as a link between human activities and the environment,create ideal conditions for the selection and spread of antibiotic resistance genes(ARGs)and antibioticresistant bacteria(ARB).Unfortunately,current treatment processes are ineffective in removing ARGs,resulting in the release of large quantities of ARB and ARGs into the aquatic environment through WWTP effluents.This,in turn,leads to their dispersion and potential transmission to human through water and the food chain.To safeguard human and environmental health,it is crucial to comprehend the mechanisms by which WWTP effluent discharge influences the distribution and diffusion of ARGs in downstream waterbodies.In this study,we examine the latest researches on the antibiotic resistome in various waterbodies that have been exposed to WWTP effluent,highlighting the key influencing mechanisms.Furthermore,recommendations for future research and management strategies to control the dissemination of ARGs from WWTPs to the environment are provided,with the aim to achieve the“One Health”objective.展开更多
Municipal wastewater consists of a downstream collection of flushed sewage(without solid waste),other household runoffs,industrial runoffs,hospital runoffs and agricultural runoffs through an underground pipe before t...Municipal wastewater consists of a downstream collection of flushed sewage(without solid waste),other household runoffs,industrial runoffs,hospital runoffs and agricultural runoffs through an underground pipe before treatment.A runoff collection system called the wastewater treatment plant(WWTPs)treats such wastewater before release into environment following specific regulatory standards.This years-long practice has been improved upon by adding end-to-end pipe technologies with a view to enhancing the quality of effluent released.However,effluents released into the environment from design/application of WWTPs appear to contain emerging contaminants of both biotic and abiotic nature.The observation of chemical contaminants,antibiotic resistant bacteria(ARB),antibiotic resistant genes(ARGs)and diverse pathogenic bacteria genera in wastewater works release further affirm the abundance of such emerging contaminants.As a result,the government and water regulatory organizations in various part of the world are considering the removal of water reuse act from recycling policy/process.Current global debate is focused on questions about sustenance of any improved additional treatment level;effect of energy consumption by added treatment stage and its impact on the environmental wellness as contaminants borne wastewater is consistently released.Technological advancement/research suggests implementation of newer innovative infrastructural systems(NIIS)such as Mobbing Bed Biofilm Rector(MBBR),for wastewater effluent management which involve addition of newer wastewater treatment stages.This review addressed current pitfalls including wastewater microbiota of high epidemiological/public health relevance and affirms the need for such improvement which requires modification of ongoing institutional framework with a view to encourage implementation of NIIS for an improved effluent release.Exploiting the advances of microbial biofilming and the potentials of microbial biofueling as discussed in various section promises a future of robust environmental system,stable operational standard,release of quality effluent and sustainable management of wastewater works.Application of the aforementioned would enhance qualityWWTPs release and in-defacto reduces spread of ARB/ARGs as well as impacts both the environment wellness and public health.展开更多
文摘This review focuses on the occurrence and interactions of engineered nanoparticles(ENPs)and brominated flame retardants(BFRs)such as polybrominated diphenyl ethers(PBDEs)in water systems and the generation of highly complex compounds in the environment.The release of ENPs and BFRs(e.g.PBDEs)to aquatic environments during their usage and disposal are summarised together with their key interaction mechanisms.The major interaction mechanisms including electrostatic,van derWaals,hydrophobic,molecular bridging and steric,hydrogen andπ-bonding,cation bridging and ligand exchange were identified.The presence of ENPs could influence the fate and behaviour of PBDEs through the interactions as well as induced reactions under certain conditions which increases the formation of complex compounds.The interaction leads to alteration of behaviour for PBDEs and their toxic effects to ecological receptors.The intermingled compound(ENPs-BFRs)would show different behaviour from the parental ENPs or BFRs,which are currently lack of investigation.This review provided insights on the interactions of ENPs and BFRs in artificial,environmental water systems and wastewater treatment plants(WWTPs),which are important for a comprehensive risk assessment.
基金supported by the National Natural Science Foundation of China(Grant No.52300243,52170185,51938001,and 52070111)the China Postdoctoral Science Foundation(No.2022M721815)the“Shuimu Tsinghua Scholar”Program of Tsinghua University,the National Treasure Ecological Research Synergetic Innovation Center,and High-Performance Computing Platform of Peking University.
文摘Effective risk assessment and control of environmental antibiotic resistance depend on comprehensive information about antibiotic resistance genes(ARGs)and their microbial hosts.Advances in sequencing technologies and bioinformatics have enabled the identification of ARG hosts using metagenomeassembled contigs and genomes.However,these approaches often suffer from information loss and require extensive computational resources.Here we introduce a bioinformatic strategy that identifies ARG hosts by prescreening ARG-like reads(ALRs)directly from total metagenomic datasets.This ALRbased method offers several advantages:(1)it enables the detection of low-abundance ARG hosts with higher accuracy in complex environments;(2)it establishes a direct relationship between the abundance of ARGs and their hosts;and(3)it reduces computation time by approximately 44e96%compared to strategies relying on assembled contigs and genomes.We applied our ALR-based strategy alongside two traditional methods to investigate a typical human-impacted environment.The results were consistent across all methods,revealing that ARGs are predominantly carried by Gammaproteobacteria and Bacilli,and their distribution patterns may indicate the impact of wastewater discharge on coastal resistome.Our strategy provides rapid and accurate identification of antibiotic-resistant bacteria,offering valuable insights for the high-throughput surveillance of environmental antibiotic resistance.This study further expands our knowledge of ARG-related risk management in future.
基金National Natural Science Foundation of China(No.51938001,51708253,and 52300243)National Key Research and Development Program of China(2023YFC3207004)。
文摘Class 1 integrons facilitate horizontal gene transfer,significantly influencing antibiotic resistance gene(ARG)dissemination within microbial communities.Wastewater treatment plants(WWTPs)are critical reservoirs of ARGs and integrons,yet the integron-mediated dynamics of ARG transfer across different WWTP types remain poorly understood.Here we show distinct ARG profiles associated with class 1 integrons in municipal and industrial WWTPs using a novel approach combining nested-like highthroughput q PCR and Pac Bio sequencing.Although industrial WWTPs contained higher absolute integron abundances,their relative ARG content was lower(1.27×10^(7)–9.59×10^(7) copies per ng integron)compared to municipal WWTPs(3.72×10^(7)–1.98×10^(8) copies per ng integron).Of the 132,084 coding sequences detected from integrons,56.8%encoded antibiotic resistance,with industrial plants showing lower ARG proportions,reduced ARG array diversity,and greater incorporation of non-ARG sequences.These findings suggest industrial WWTP integrons integrate a broader array of exogenous genes,reflecting adaptation to complex wastewater compositions.This work enhances our understanding of integron-driven ARG dynamics in wastewater and offers a robust strategy for environmental integron analysis.
基金supported by the National Natural Science Foundation of China(Grant Nos.51938001,52170185 and 52070111)the China Postdoctoral Science Foundation(No.2022M721815)。
文摘Antimicrobial resistance(AMR)has emerged as a significant challenge in human health.Wastewater treatment plants(WWTPs),acting as a link between human activities and the environment,create ideal conditions for the selection and spread of antibiotic resistance genes(ARGs)and antibioticresistant bacteria(ARB).Unfortunately,current treatment processes are ineffective in removing ARGs,resulting in the release of large quantities of ARB and ARGs into the aquatic environment through WWTP effluents.This,in turn,leads to their dispersion and potential transmission to human through water and the food chain.To safeguard human and environmental health,it is crucial to comprehend the mechanisms by which WWTP effluent discharge influences the distribution and diffusion of ARGs in downstream waterbodies.In this study,we examine the latest researches on the antibiotic resistome in various waterbodies that have been exposed to WWTP effluent,highlighting the key influencing mechanisms.Furthermore,recommendations for future research and management strategies to control the dissemination of ARGs from WWTPs to the environment are provided,with the aim to achieve the“One Health”objective.
文摘Municipal wastewater consists of a downstream collection of flushed sewage(without solid waste),other household runoffs,industrial runoffs,hospital runoffs and agricultural runoffs through an underground pipe before treatment.A runoff collection system called the wastewater treatment plant(WWTPs)treats such wastewater before release into environment following specific regulatory standards.This years-long practice has been improved upon by adding end-to-end pipe technologies with a view to enhancing the quality of effluent released.However,effluents released into the environment from design/application of WWTPs appear to contain emerging contaminants of both biotic and abiotic nature.The observation of chemical contaminants,antibiotic resistant bacteria(ARB),antibiotic resistant genes(ARGs)and diverse pathogenic bacteria genera in wastewater works release further affirm the abundance of such emerging contaminants.As a result,the government and water regulatory organizations in various part of the world are considering the removal of water reuse act from recycling policy/process.Current global debate is focused on questions about sustenance of any improved additional treatment level;effect of energy consumption by added treatment stage and its impact on the environmental wellness as contaminants borne wastewater is consistently released.Technological advancement/research suggests implementation of newer innovative infrastructural systems(NIIS)such as Mobbing Bed Biofilm Rector(MBBR),for wastewater effluent management which involve addition of newer wastewater treatment stages.This review addressed current pitfalls including wastewater microbiota of high epidemiological/public health relevance and affirms the need for such improvement which requires modification of ongoing institutional framework with a view to encourage implementation of NIIS for an improved effluent release.Exploiting the advances of microbial biofilming and the potentials of microbial biofueling as discussed in various section promises a future of robust environmental system,stable operational standard,release of quality effluent and sustainable management of wastewater works.Application of the aforementioned would enhance qualityWWTPs release and in-defacto reduces spread of ARB/ARGs as well as impacts both the environment wellness and public health.
