Despite their biodegradability and economic advantage, plant leaves used as packaging can constitute a public health problem. The aim of this study was to characterize the microbial diversity contaminating plant leave...Despite their biodegradability and economic advantage, plant leaves used as packaging can constitute a public health problem. The aim of this study was to characterize the microbial diversity contaminating plant leaves used as food packaging. In total, two hundred and forty (240) samples composed of Thaumatococcus daniellii and Musa paradisiaca leaves were collected and analyzed. Microbial diversity was assessed using specific medium and biochemical tests. The resistance profile was determined by the Müeller-Hinton agar diffusion method. The resistance (blaSHV, blaIMP, blaTEM) and biofilm formation (pslA, pelA) genes were searched by PCR method. Plant leaves were contaminated by bacterial (68.7%) and fungal (100%) strains. Extreme bacterial (7.1 log10 cfu/cm2) and fungal (3.5 log10 cfu/cm2) loads were obtained on Thaumatococcus daniellii leaves. Bacterial prevalence was 45.1% (S. aureus), 38.8% (E. coli) and 16.1 (P. aeruginosa). In order of decreasing importance, the prevalence of fungal species was 41.1% (A. flavus), 33.1% (A. fumigattus), 13.7% (A. niger) and 12.1% Candida sp. Resistance of E. coli to penicillins ranges from 31.6% to 87.3% and to cephalosporins from 13.3% to 28%. The P. aeruginosa strains were mainly resistant to aztreonam (87.6%). Those of S. aureus showed resistance to tetracycline (67.6), vancomycin (53), erythromycin (44.6) and levofloxacin (32.7). The blaSHV (14.28% to 18.60%) and blaIMP (9.52% to 16.28%) genes were detected in the bacterial strains. P. aeruginosa strains (19.05%) harbored the pslA and pelA genes. The health safety of these biodegradable plant-based packaging contributes to their valorization.展开更多
香蕉品种‘Agbagaba’和‘Orishele’的胚性细胞悬浮系(ECS)在液体培养基中分别预培养1和2周后,将其接种在RD1和M3培养基上,于光照或黑暗条件下进行体胚的再生。从沉积细胞体积(SCV) 为1 mL(1 mL SCV)的ECS获得的再生体胚数量因预培养...香蕉品种‘Agbagaba’和‘Orishele’的胚性细胞悬浮系(ECS)在液体培养基中分别预培养1和2周后,将其接种在RD1和M3培养基上,于光照或黑暗条件下进行体胚的再生。从沉积细胞体积(SCV) 为1 mL(1 mL SCV)的ECS获得的再生体胚数量因预培养时间、再生培养基的种类及培养条件的不同而异。植株的再生率及从1 mL SCV的ECS获得的再生植株数量也受上述体胚再生条件的间接影响。展开更多
植物光系统Ⅰ反应中心亚基Ⅴ(photosystemⅠreaction center subunitⅤ,简称PSAG或PSⅠ-G)是光合系统Ⅰ的主要组件,具有维持PSⅠ复合体稳定性的重要作用,并与抗盐密切相关。本研究以福州宦溪野生蕉(Musa spp.AB group)叶片为材料,采用...植物光系统Ⅰ反应中心亚基Ⅴ(photosystemⅠreaction center subunitⅤ,简称PSAG或PSⅠ-G)是光合系统Ⅰ的主要组件,具有维持PSⅠ复合体稳定性的重要作用,并与抗盐密切相关。本研究以福州宦溪野生蕉(Musa spp.AB group)叶片为材料,采用同源克隆的方法 ,首次分离到PSAG基因的2个成员:PSAG1、PSAG2(GenBank登录号分别为JX317082、JX317083),分别为800、827 bp,分别编码150、160个氨基酸;PSAG1、PSAG2的基因组序列分析表明2个成员均没有内含子。生物信息学分析表明:PSAG1、PSAG2具有PSⅠ的Ⅹ亚基超家族(photosystemⅠreaction center subunitⅩpsaK)保守结构域,是不具有信号肽的跨膜蛋白,具有亲水性;PSAG1、PSAG2均有4个位点发生磷酸化。宦溪野生蕉PSAG在进化过程中形成了特殊的结构特征,即PSAG1和PSAG2没有内含子,并且在不同物种间保守区具有高度的一致性,为保持PSAG功能的稳定性提供了重要保证。展开更多
The center of diversity of the genus Musa （Musaceae） is in Southeast Asia, a region not studied in detail and where new species and varieties continue to be reported. A new wild banana species, M. chunii Hakkinen fr...The center of diversity of the genus Musa （Musaceae） is in Southeast Asia, a region not studied in detail and where new species and varieties continue to be reported. A new wild banana species, M. chunii Hakkinen from Yunnan, China is described and illustrated based on observed morphological characteristics in the field. This extremely rare new species was only found in Tongbiguan Nature Reserve, Dehong District, West Yunnan. A key to M. chunii and related taxa is provided. In addition, critical notes regarding M. rubra Kurz identity are given.展开更多
Objective:To invesligale the hepatoprotective activity of stem of Musa paradisiaca(M.paradisiaca)in CCl_4 and paracetamol induced hepatotoxicity models in rats.Methods:Hepatoprotective activity of alcoholic and aqueou...Objective:To invesligale the hepatoprotective activity of stem of Musa paradisiaca(M.paradisiaca)in CCl_4 and paracetamol induced hepatotoxicity models in rats.Methods:Hepatoprotective activity of alcoholic and aqueous extracts of stem of M.paradisiaca was demonstrated by using two experimentally induced hepatotoxicity models.Results:Administration of hepatotoxins(CCl_4 and paracetamol)showed significant biochemical and histological deteriorations in the liver of experimental animals.Pretreatment with alcoholic extract(500 mg/kg),more significantly and to a lesser extent the alcoholic extract(250 mg/kg)and aqueous extract(500 mg/kg),reduced the elevated levels of the serum enzymes like serum glutamic-oxaloacetic transaminase(SCOT),serum glutamic pyruvic transaminase(SGPT),alkaline phosphatase(ALP)and bilirubin levels and alcoholic and aqueous extracts reversed the hepatic damage towards the normal,which further evidenced the hepatoprotective activity of stem of M.paradisiaca.Conclusions:The alcoholic extract at doses of 250 and 500 mg/kg,p.o.and aqueous extract at a dose of 500 mg/kg,p.o.of stem of M.paradisiaca have significant effect on the liver of CCl_4 and paracetamol induced hepatotoxicity animal models.展开更多
文摘Despite their biodegradability and economic advantage, plant leaves used as packaging can constitute a public health problem. The aim of this study was to characterize the microbial diversity contaminating plant leaves used as food packaging. In total, two hundred and forty (240) samples composed of Thaumatococcus daniellii and Musa paradisiaca leaves were collected and analyzed. Microbial diversity was assessed using specific medium and biochemical tests. The resistance profile was determined by the Müeller-Hinton agar diffusion method. The resistance (blaSHV, blaIMP, blaTEM) and biofilm formation (pslA, pelA) genes were searched by PCR method. Plant leaves were contaminated by bacterial (68.7%) and fungal (100%) strains. Extreme bacterial (7.1 log10 cfu/cm2) and fungal (3.5 log10 cfu/cm2) loads were obtained on Thaumatococcus daniellii leaves. Bacterial prevalence was 45.1% (S. aureus), 38.8% (E. coli) and 16.1 (P. aeruginosa). In order of decreasing importance, the prevalence of fungal species was 41.1% (A. flavus), 33.1% (A. fumigattus), 13.7% (A. niger) and 12.1% Candida sp. Resistance of E. coli to penicillins ranges from 31.6% to 87.3% and to cephalosporins from 13.3% to 28%. The P. aeruginosa strains were mainly resistant to aztreonam (87.6%). Those of S. aureus showed resistance to tetracycline (67.6), vancomycin (53), erythromycin (44.6) and levofloxacin (32.7). The blaSHV (14.28% to 18.60%) and blaIMP (9.52% to 16.28%) genes were detected in the bacterial strains. P. aeruginosa strains (19.05%) harbored the pslA and pelA genes. The health safety of these biodegradable plant-based packaging contributes to their valorization.
文摘香蕉品种‘Agbagaba’和‘Orishele’的胚性细胞悬浮系(ECS)在液体培养基中分别预培养1和2周后,将其接种在RD1和M3培养基上,于光照或黑暗条件下进行体胚的再生。从沉积细胞体积(SCV) 为1 mL(1 mL SCV)的ECS获得的再生体胚数量因预培养时间、再生培养基的种类及培养条件的不同而异。植株的再生率及从1 mL SCV的ECS获得的再生植株数量也受上述体胚再生条件的间接影响。
文摘植物光系统Ⅰ反应中心亚基Ⅴ(photosystemⅠreaction center subunitⅤ,简称PSAG或PSⅠ-G)是光合系统Ⅰ的主要组件,具有维持PSⅠ复合体稳定性的重要作用,并与抗盐密切相关。本研究以福州宦溪野生蕉(Musa spp.AB group)叶片为材料,采用同源克隆的方法 ,首次分离到PSAG基因的2个成员:PSAG1、PSAG2(GenBank登录号分别为JX317082、JX317083),分别为800、827 bp,分别编码150、160个氨基酸;PSAG1、PSAG2的基因组序列分析表明2个成员均没有内含子。生物信息学分析表明:PSAG1、PSAG2具有PSⅠ的Ⅹ亚基超家族(photosystemⅠreaction center subunitⅩpsaK)保守结构域,是不具有信号肽的跨膜蛋白,具有亲水性;PSAG1、PSAG2均有4个位点发生磷酸化。宦溪野生蕉PSAG在进化过程中形成了特殊的结构特征,即PSAG1和PSAG2没有内含子,并且在不同物种间保守区具有高度的一致性,为保持PSAG功能的稳定性提供了重要保证。
文摘The center of diversity of the genus Musa （Musaceae） is in Southeast Asia, a region not studied in detail and where new species and varieties continue to be reported. A new wild banana species, M. chunii Hakkinen from Yunnan, China is described and illustrated based on observed morphological characteristics in the field. This extremely rare new species was only found in Tongbiguan Nature Reserve, Dehong District, West Yunnan. A key to M. chunii and related taxa is provided. In addition, critical notes regarding M. rubra Kurz identity are given.
文摘Objective:To invesligale the hepatoprotective activity of stem of Musa paradisiaca(M.paradisiaca)in CCl_4 and paracetamol induced hepatotoxicity models in rats.Methods:Hepatoprotective activity of alcoholic and aqueous extracts of stem of M.paradisiaca was demonstrated by using two experimentally induced hepatotoxicity models.Results:Administration of hepatotoxins(CCl_4 and paracetamol)showed significant biochemical and histological deteriorations in the liver of experimental animals.Pretreatment with alcoholic extract(500 mg/kg),more significantly and to a lesser extent the alcoholic extract(250 mg/kg)and aqueous extract(500 mg/kg),reduced the elevated levels of the serum enzymes like serum glutamic-oxaloacetic transaminase(SCOT),serum glutamic pyruvic transaminase(SGPT),alkaline phosphatase(ALP)and bilirubin levels and alcoholic and aqueous extracts reversed the hepatic damage towards the normal,which further evidenced the hepatoprotective activity of stem of M.paradisiaca.Conclusions:The alcoholic extract at doses of 250 and 500 mg/kg,p.o.and aqueous extract at a dose of 500 mg/kg,p.o.of stem of M.paradisiaca have significant effect on the liver of CCl_4 and paracetamol induced hepatotoxicity animal models.
