In order to contribute to a better understanding of the biodiversity of local chicken populations, this study focused on the description of the essential qualitative parameters in the phenotypic characterization of lo...In order to contribute to a better understanding of the biodiversity of local chicken populations, this study focused on the description of the essential qualitative parameters in the phenotypic characterization of local species. Conducted in 6 localities in the Far North Region of Cameroon (Doukoula, Yagoua, Guidiguis, Dziguilao, Maroua 3rd and Méri), a population of 240 local chickens, including 172 hens and 68 cocks were characterized in family farms. The choice of localities was made on the basis of their strong potential local chicken flocks in the region. To this end, each animal was the subject of a qualitative description based on the identification of the sex of the animal, the description of the colorations of the plumage and extremities, and the description of the types of format, plumage and crests. All observations were made with the naked eye and in daylight and then photographed. The main results show that the plumage colors are multiple and dominated by the White-Pied-Black (21.7%) and the Mille-fleur (20.8%);the wattles are dominated by the red (60%) and the pink (35.4%);the tarsi are dominated by the white (43.8%) and the black (32.08%);the white skin (92.5%) dominates over the pink skin (7.5%);the “Smooth-uniform” feather is dominant (97.08%), followed by the smooth-crested type (2.08%) and the fries type (0.82%);the medium size of the chickens is dominant (86.66%), followed by the dwarf size (9.58%) and the large size (3.75%). The results of this study demonstrate that there is a strong phenotypic diversity within the local chicken population. This diversity can serve as a basis for the development of selection, conservation and genetic potential improvement programs based on rational exploitation of the local chicken.展开更多
Malaria is a potentially lethal disease caused by parasites of the Plasmodium genus, transmitted to humans through the bite of infected female mosquitoes, primarily Anopheles. To control this disease, various strategi...Malaria is a potentially lethal disease caused by parasites of the Plasmodium genus, transmitted to humans through the bite of infected female mosquitoes, primarily Anopheles. To control this disease, various strategies are implemented, including biological control, which targets the vectors of the parasite. This approach uses biological agents such as entomopathogenic fungi, including Metarhizium pingshaense, a fungus capable of causing lethal infections in mosquitoes. The production of Metarhizium pingshaense is still limited in Burkina Faso, and local cultivation of this fungus could help fill this gap. A study was conducted to identify optimal local substrates that promote its growth. Indeed, after gathering information on the dietary habits of populations in Bobo-Dioulasso and Bama, three potential substrates were selected: rice, cornmeal dough (MFL), and beans. These substrates were inoculated with two strains of Metarhizium pingshaense (S10 and S26) to assess their ability to support fungal growth and their effectiveness. Experimental results showed that MFL and bean substrates favored optimal growth of Metarhizium pingshaense, with growths of 1.91 cm and 2.13 cm after 8 days, compared to 1.83 cm on a standard media (PDA). In terms of virulence, S26 strain caused 60% mosquito mortality on both the bean and PDA media, while S10 strain induced mortalities of 50% for bean and 62% for PDA.展开更多
【目的】制备番鸭丝裂原活化蛋白激酶1(mitogen-activated protein kinase,MAPK1)蛋白多克隆抗体,鉴定其特异性与适用性,并探究MAPK1蛋白在卵泡颗粒细胞及卵泡中的表达定位,为研究其在番鸭卵泡发育与生殖调控中的功能提供试验工具。【...【目的】制备番鸭丝裂原活化蛋白激酶1(mitogen-activated protein kinase,MAPK1)蛋白多克隆抗体,鉴定其特异性与适用性,并探究MAPK1蛋白在卵泡颗粒细胞及卵泡中的表达定位,为研究其在番鸭卵泡发育与生殖调控中的功能提供试验工具。【方法】以番鸭卵巢组织cDNA为模板,通过PCR扩增MAPK1基因并测序,运用生物信息学工具分析MAPK1蛋白理化性质及主要抗原表位,筛选免疫原区段。对免疫原序列进行大肠杆菌密码子优化并人工合成,经同源重组克隆至原核表达载体pET-30a(+),构建重组表达载体pET30a-MAPK1并转化大肠杆菌BL21(DE3)感受态细胞。在HB-PET自诱导培养基中表达、纯化MAPK1重组蛋白,将纯化蛋白与QuickAntibody-Rabbit8W佐剂混合后免疫新西兰白兔制备多克隆抗体。采用ELISA、Western blotting、细胞免疫荧光(CIF)、组织免疫荧光(TIF)和免疫组化(IHC)等方法检测多克隆抗体的效价、特异性与适用性,并以颗粒细胞标志物促卵泡激素受体(FSHR)为参照分析MAPK1表达定位。【结果】成功克隆了番鸭MAPK1基因,大小约1107 bp,编码368个氨基酸。MAPK1蛋白理论分子质量约42 ku。生物信息学预测显示,MAPK1蛋白不含信号肽和跨膜结构;第50—100、200—300和250—350位氨基酸区段亲水性良好,第30—90和200—300氨基酸区段抗原指数较高,第30—70、120—150、200—260及320—360位氨基酸区段的氨基酸表面暴露概率较大。MAPK1蛋白二级结构以α-螺旋为主,包含典型激酶保守结构,活化环呈无规则卷曲状态,主要定位于细胞质和细胞核。综合蛋白特性,最终筛选了第4—364位氨基酸作为候选免疫原区段。成功构建原核重组表达载体pET30-MAPK1,MAPK1重组蛋白以可溶性形式表达,分子质量约48 ku。成功制备了兔抗鸭MAPK1蛋白多克隆抗体,ELISA结果显示该抗体效价达1∶102400;Western blotting、CIF、TIF和IHC结果显示,制备的多克隆抗体可特异识别MAPK1重组蛋白及番鸭卵泡颗粒细胞和卵泡中的内源性MAPK1蛋白。在颗粒细胞中,MAPK1主要定位于卵泡颗粒细胞的细胞质和细胞核;在卵泡中,MAPK1主要分布于卵泡颗粒细胞层,表达定位与FSHR基本一致。【结论】试验成功制备了番鸭MAPK1多克隆抗体,该抗体特异性良好,适用于番鸭卵泡相关细胞与组织样本中MAPK1的免疫学检测。研究结果为MAPK1在卵泡发育及生殖调控中的功能研究提供了技术支撑。展开更多
为分析集成三维点云逆向建模方法(integrated 3D point cloud reverse modeling,IPCRM)在建立薄厚型钢构件三维模型时的精度表现,以局部变形角钢为研究对象,利用SfM(structure from motion)-MVS(multi-view stereo)算法建立其三维点云模...为分析集成三维点云逆向建模方法(integrated 3D point cloud reverse modeling,IPCRM)在建立薄厚型钢构件三维模型时的精度表现,以局部变形角钢为研究对象,利用SfM(structure from motion)-MVS(multi-view stereo)算法建立其三维点云模型,借助逆向建模技术生成曲面模型,重点开展了模型精度验证试验.结果表明:各表面形状特征参数的相对误差均在8%以内(吻合度验证);4种角钢模型与实际角钢间无显著性差异(P值,P=0.99),且角钢厚度对模型精度无显著性影响(P值,P=0.95),结论在95%的置信度水平下成立(差异显著性验证).研究结果为后续算法优化及利用此类方法进行合理的钢构件局部变形损伤检测与承载性能评价提供依据.展开更多
针对弱纹理和变光照环境下基于点特征的视觉SLAM(simultaneous localization and mapping)算法轨迹漂移的问题,提出了一种基于改进自适应阈值ELSED算法(Adaptive-ELSED)的快速点线融合双目视觉SLAM算法。通过在ELSED算法中添加自适应阈...针对弱纹理和变光照环境下基于点特征的视觉SLAM(simultaneous localization and mapping)算法轨迹漂移的问题,提出了一种基于改进自适应阈值ELSED算法(Adaptive-ELSED)的快速点线融合双目视觉SLAM算法。通过在ELSED算法中添加自适应阈值矩阵,动态调整不同光照条件下梯度阈值,并使用长度抑制和短线合并策略,提高线特征的质量。利用基于双目几何约束和图像结构相似性(SSIM)进行快速线段特征三角化。基于历史位姿及误差分析获取初始位姿,通过自适应因子实现光束法平差过程中点线特征的更有效融合。实验结果表明,所提算法在提高线特征质量的同时,耗时仅为LSD算法的50%,线特征匹配速度较传统LBD算法提升67%,挑战性场景下轨迹误差较ORB-SLAM3降低62.2%,系统的平均跟踪帧率为27帧/s,在保证系统实时性的同时,显著提升了系统在弱纹理、变光照环境下的精度和鲁棒性。展开更多
文摘In order to contribute to a better understanding of the biodiversity of local chicken populations, this study focused on the description of the essential qualitative parameters in the phenotypic characterization of local species. Conducted in 6 localities in the Far North Region of Cameroon (Doukoula, Yagoua, Guidiguis, Dziguilao, Maroua 3rd and Méri), a population of 240 local chickens, including 172 hens and 68 cocks were characterized in family farms. The choice of localities was made on the basis of their strong potential local chicken flocks in the region. To this end, each animal was the subject of a qualitative description based on the identification of the sex of the animal, the description of the colorations of the plumage and extremities, and the description of the types of format, plumage and crests. All observations were made with the naked eye and in daylight and then photographed. The main results show that the plumage colors are multiple and dominated by the White-Pied-Black (21.7%) and the Mille-fleur (20.8%);the wattles are dominated by the red (60%) and the pink (35.4%);the tarsi are dominated by the white (43.8%) and the black (32.08%);the white skin (92.5%) dominates over the pink skin (7.5%);the “Smooth-uniform” feather is dominant (97.08%), followed by the smooth-crested type (2.08%) and the fries type (0.82%);the medium size of the chickens is dominant (86.66%), followed by the dwarf size (9.58%) and the large size (3.75%). The results of this study demonstrate that there is a strong phenotypic diversity within the local chicken population. This diversity can serve as a basis for the development of selection, conservation and genetic potential improvement programs based on rational exploitation of the local chicken.
文摘Malaria is a potentially lethal disease caused by parasites of the Plasmodium genus, transmitted to humans through the bite of infected female mosquitoes, primarily Anopheles. To control this disease, various strategies are implemented, including biological control, which targets the vectors of the parasite. This approach uses biological agents such as entomopathogenic fungi, including Metarhizium pingshaense, a fungus capable of causing lethal infections in mosquitoes. The production of Metarhizium pingshaense is still limited in Burkina Faso, and local cultivation of this fungus could help fill this gap. A study was conducted to identify optimal local substrates that promote its growth. Indeed, after gathering information on the dietary habits of populations in Bobo-Dioulasso and Bama, three potential substrates were selected: rice, cornmeal dough (MFL), and beans. These substrates were inoculated with two strains of Metarhizium pingshaense (S10 and S26) to assess their ability to support fungal growth and their effectiveness. Experimental results showed that MFL and bean substrates favored optimal growth of Metarhizium pingshaense, with growths of 1.91 cm and 2.13 cm after 8 days, compared to 1.83 cm on a standard media (PDA). In terms of virulence, S26 strain caused 60% mosquito mortality on both the bean and PDA media, while S10 strain induced mortalities of 50% for bean and 62% for PDA.
文摘为分析集成三维点云逆向建模方法(integrated 3D point cloud reverse modeling,IPCRM)在建立薄厚型钢构件三维模型时的精度表现,以局部变形角钢为研究对象,利用SfM(structure from motion)-MVS(multi-view stereo)算法建立其三维点云模型,借助逆向建模技术生成曲面模型,重点开展了模型精度验证试验.结果表明:各表面形状特征参数的相对误差均在8%以内(吻合度验证);4种角钢模型与实际角钢间无显著性差异(P值,P=0.99),且角钢厚度对模型精度无显著性影响(P值,P=0.95),结论在95%的置信度水平下成立(差异显著性验证).研究结果为后续算法优化及利用此类方法进行合理的钢构件局部变形损伤检测与承载性能评价提供依据.
文摘针对弱纹理和变光照环境下基于点特征的视觉SLAM(simultaneous localization and mapping)算法轨迹漂移的问题,提出了一种基于改进自适应阈值ELSED算法(Adaptive-ELSED)的快速点线融合双目视觉SLAM算法。通过在ELSED算法中添加自适应阈值矩阵,动态调整不同光照条件下梯度阈值,并使用长度抑制和短线合并策略,提高线特征的质量。利用基于双目几何约束和图像结构相似性(SSIM)进行快速线段特征三角化。基于历史位姿及误差分析获取初始位姿,通过自适应因子实现光束法平差过程中点线特征的更有效融合。实验结果表明,所提算法在提高线特征质量的同时,耗时仅为LSD算法的50%,线特征匹配速度较传统LBD算法提升67%,挑战性场景下轨迹误差较ORB-SLAM3降低62.2%,系统的平均跟踪帧率为27帧/s,在保证系统实时性的同时,显著提升了系统在弱纹理、变光照环境下的精度和鲁棒性。
