The present study analyzed the predictive value of combined analysis of collapsin response mediator protein 4 （CRMP4） methylation levels and the Cancer of the Prostate Risk Assessment （CAPRA-S） Postsurgical score ...The present study analyzed the predictive value of combined analysis of collapsin response mediator protein 4 （CRMP4） methylation levels and the Cancer of the Prostate Risk Assessment （CAPRA-S） Postsurgical score of patients who required adjuvant hormone therapy （AHT） after radical prostatectomy （RP）, We retrospectively analyzed 305 patients with prostate cancer （PCa） who received RP and subsequent androgen deprivation therapy （ADT）. Two hundred and thirty patients with clinically high-risk PCa underwent immediate ADT, and 75 patients with intermediate risk PCa underwent deferred ADT. CRMP4 methylation levels in biopsies were determined, and CAPRA-S scores were calculated. In the deferred ADT group, the values of the hazard ratios for tumor progression and cancer-specific mortality （CSM） in patients with 〉15% CRMP4 methylation were 6.81 （95% CI, 2.34-19.80） and 12.83 （95% CI. 2.16-26.10）, respectively. Receiver-operating characteristic curve analysis indicated that CRMP4 methylation levels 〉15% served as a significant prognostic marker of tumor progression and CSM. In the immediate ADT group, CAPRA-S scores ：〉6 and CRMP4 methylation levels ：〉15% were independent predictors of these outcomes （uni- and multi-variable Cox regression analyses）. The differences in the 5-year progression-free survival between each combination were statistically significant. Combining CAPRA-S score and CRMP4 methylation levels improved the area under the curve compared with the CRMP4 or CAPRA-S model. Therefore, CRMP4 methylation levels ：〉15% were significantly associated with a poor prognosis and their combination with CAPRA-S score accurately predicted tumor progression and metastasis for patients requiring AHT after RP.展开更多
Skeletal muscle accounts for about 40% of mammalian body weight, the development of which is a dynamic, complex and precisely regulated process that is critical for meat production. We here described the transcriptome...Skeletal muscle accounts for about 40% of mammalian body weight, the development of which is a dynamic, complex and precisely regulated process that is critical for meat production. We here described the transcriptome expression profile in 21 goat samples collected at 7 growth stages from fetus to kid, including fetal 45(F45), 65(F65), 90(F90), 120(F120), and 135(F135) days, and birth 1(B1) day and 90(B90) days kids. Paraffin sections combined with RNA-seq data of the 7 stages divided the transcriptomic functions of skeletal muscle into 4 states: before F90, F120, F135 and B1, and B90. And the dynamic expression of all 4 793 differentially expressed genes(DEGs) was identified. Furthermore, DEGs were clustered by weighted gene correlation network analysis into 4 modules(turquoise, grey, blue and brown) that corresponded to these 4 states. Functional and pathway analysis indicated that the active genes in the stages before F90(turquoise) were closely related to skeletal muscle proliferation. The DEGs in the F120-related module(grey) were found to participate in the regulation of skeletal muscle structure and skeletal muscle development by regulating t RNA. The brown module(F135 and B1) regulated fatty acid biological processes to maintain the normal development of muscle cells. The DEGs of B90 high correlation module(blue) were involved the strengthening and power of skeletal muscle through the regulation of actin filaments and tropomyosin. Our current data thus revealed the internal functional conversion of the goat skeletal muscle in the growth from fetus to kid. The results provided a theoretical basis for analyzing the involvement of m RNA in skeletal muscle development.展开更多
Amino acids are an important type of bioactive components in Chinese traditional medicines, especially animal drugs. However, few modern analytical methods targeted at amino acids have been developed for the quality c...Amino acids are an important type of bioactive components in Chinese traditional medicines, especially animal drugs. However, few modern analytical methods targeted at amino acids have been developed for the quality control of animal drugs. A gas chromatography-mass spectrometric method was built for the quantification analysis of amino acids in the extracts of Comus Caprae Hircus which has been widely used in Chinese medicine compound prescriptions. The full method validation, including the linearity, limit of detection and quantification, repeatability, precision, stability, and recovery test, was examined. The results indicate that the developed method is suitable for the quantification of amino acids in the extracts of Comus Caprae Hircus. The developed method was applied to the quantification analysis of twelve amino acids in different batches of extracts of Comus Caprae Hircus.展开更多
The aim of this project was to study indigenous breeding practices used in management of Capra hircus (Galla and Small East African goats) populations in Kajiado County in Rift valley province and Makueni County in Ea...The aim of this project was to study indigenous breeding practices used in management of Capra hircus (Galla and Small East African goats) populations in Kajiado County in Rift valley province and Makueni County in Eastern province. Data were obtained through a field survey using questionnaires (Appendix I) and personal observations between 1st September, 2013 and 2nd December, 2013. This study covered key characteristics of goats production and areas of goat breeding, such as general farm details, number of goats, main activities of the farmers, farming types, breeds, flock structure, feeding, housing, catastrophes, selection, mating systems, breeding systems, average age at parturition, breeding problems, and the importance of goats. Results showed that the average number of goats in Kajiado was 100.65 ± std 49.88 while in Makueni it was 12.28 ± std 6.46. The main activity of the people interviewed was farming as 58 people (96.67%) in Kajiado and 42 people (61.60%) chose farming as their main activity because this was their main source of livelihood. Flocks were dominated by breeding females at a mean of 39.06 ± std 16.75 in Kajiado and a mean of 5.62 ± std 3.50 in Makueni because females were kept to reproduce to increase the size of the flock and the males were kept majorly for cash and only one or two were left to reproduce with the females. Drought was the major catastrophe as it killed an average number of goats of 6.33 ± std 4.36. Pneumonia and diarrhoea were the major diseases according to 28 farmers (46.66%) in Kajiado and 31 farmers (51.66%) in Makueni. Ticks and fleas were the major parasites according to 42 farmers (70%) in Kajiado and 4 farmers (63.34%) in Makueni. Treatment was mostly done by the farmers individually as 54 farmers (90%) in Kajiado and 46 farmers (76.67%) in Makueni treated the animals by themselves. This was so because it was either not easy to get a veterinarian or expensive for them to hire veterinarian doctors. Some farmers used traditional medicine like mavuavui;Steganotaenia araliacea was used to treat pneumonia. Farmers also devised feeding methods during drought as 48 farmers (80.00%) in Kajiado and 23 farmers (38.33%) in Makueni cut leaves from up trees to feed the goats. When doing selection of breed, 58 farmers (96.67%) and 57 farmers (95%) considered large body size and drought resistance respectively in Kajiado. The farmers in Makueni considered age and drought resistance at equal chances of 59 farmers (98.33%). The main mating system was naturally uncontrolled as 113 farmers (95.17%) of the overall 120 farmers interviewed in Kajiado and Makueni chose this as the main mating method. The major breeding system was pure breeding at 85 farmers (70.83%). The average age at parturition of the goats was 1.435 ± 0.125 years in Kajiado and 1.44 ± 0.121 years in Makueni. Abortion was the major breeding problem because it was caused by environmental stressors like drought and diseases as 54 farmers (93.92%) in Kajiado and 55 farmers (95.66%) claimed that it was a problem. Goats were majorly kept for cash (100%) and meat (100%) in Kajiado and for cash (100%) and dowry (100%) in Makueni. In conclusion, the study showed that crossbreeding was encouraged by mating that occurred at the markets, water points, free ranging feeding method, pastoralism due to drought, selection methods and translocation of female goats from Makueni County to be mated with the males in Kajiado County. Environmental problems like drought and diseases caused several deaths and reduced the level of existing gene pool of the goats.展开更多
文摘The present study analyzed the predictive value of combined analysis of collapsin response mediator protein 4 （CRMP4） methylation levels and the Cancer of the Prostate Risk Assessment （CAPRA-S） Postsurgical score of patients who required adjuvant hormone therapy （AHT） after radical prostatectomy （RP）, We retrospectively analyzed 305 patients with prostate cancer （PCa） who received RP and subsequent androgen deprivation therapy （ADT）. Two hundred and thirty patients with clinically high-risk PCa underwent immediate ADT, and 75 patients with intermediate risk PCa underwent deferred ADT. CRMP4 methylation levels in biopsies were determined, and CAPRA-S scores were calculated. In the deferred ADT group, the values of the hazard ratios for tumor progression and cancer-specific mortality （CSM） in patients with 〉15% CRMP4 methylation were 6.81 （95% CI, 2.34-19.80） and 12.83 （95% CI. 2.16-26.10）, respectively. Receiver-operating characteristic curve analysis indicated that CRMP4 methylation levels 〉15% served as a significant prognostic marker of tumor progression and CSM. In the immediate ADT group, CAPRA-S scores ：〉6 and CRMP4 methylation levels ：〉15% were independent predictors of these outcomes （uni- and multi-variable Cox regression analyses）. The differences in the 5-year progression-free survival between each combination were statistically significant. Combining CAPRA-S score and CRMP4 methylation levels improved the area under the curve compared with the CRMP4 or CAPRA-S model. Therefore, CRMP4 methylation levels ：〉15% were significantly associated with a poor prognosis and their combination with CAPRA-S score accurately predicted tumor progression and metastasis for patients requiring AHT after RP.
基金supported by the National Natural Science Foundation of China (31772566 and 31972629)the Anhui Key Research and Development Program, China (1804a07020128)the Anhui Province Academic and Technology Leader Reserve Talent Project, China (2019H206)。
文摘Skeletal muscle accounts for about 40% of mammalian body weight, the development of which is a dynamic, complex and precisely regulated process that is critical for meat production. We here described the transcriptome expression profile in 21 goat samples collected at 7 growth stages from fetus to kid, including fetal 45(F45), 65(F65), 90(F90), 120(F120), and 135(F135) days, and birth 1(B1) day and 90(B90) days kids. Paraffin sections combined with RNA-seq data of the 7 stages divided the transcriptomic functions of skeletal muscle into 4 states: before F90, F120, F135 and B1, and B90. And the dynamic expression of all 4 793 differentially expressed genes(DEGs) was identified. Furthermore, DEGs were clustered by weighted gene correlation network analysis into 4 modules(turquoise, grey, blue and brown) that corresponded to these 4 states. Functional and pathway analysis indicated that the active genes in the stages before F90(turquoise) were closely related to skeletal muscle proliferation. The DEGs in the F120-related module(grey) were found to participate in the regulation of skeletal muscle structure and skeletal muscle development by regulating t RNA. The brown module(F135 and B1) regulated fatty acid biological processes to maintain the normal development of muscle cells. The DEGs of B90 high correlation module(blue) were involved the strengthening and power of skeletal muscle through the regulation of actin filaments and tropomyosin. Our current data thus revealed the internal functional conversion of the goat skeletal muscle in the growth from fetus to kid. The results provided a theoretical basis for analyzing the involvement of m RNA in skeletal muscle development.
基金Supported by the National Basic Research Program of China(No.2005CB523402) the Program for New Century Excellent Talents in University of China(No.NCET-06-0515)
文摘Amino acids are an important type of bioactive components in Chinese traditional medicines, especially animal drugs. However, few modern analytical methods targeted at amino acids have been developed for the quality control of animal drugs. A gas chromatography-mass spectrometric method was built for the quantification analysis of amino acids in the extracts of Comus Caprae Hircus which has been widely used in Chinese medicine compound prescriptions. The full method validation, including the linearity, limit of detection and quantification, repeatability, precision, stability, and recovery test, was examined. The results indicate that the developed method is suitable for the quantification of amino acids in the extracts of Comus Caprae Hircus. The developed method was applied to the quantification analysis of twelve amino acids in different batches of extracts of Comus Caprae Hircus.
文摘The aim of this project was to study indigenous breeding practices used in management of Capra hircus (Galla and Small East African goats) populations in Kajiado County in Rift valley province and Makueni County in Eastern province. Data were obtained through a field survey using questionnaires (Appendix I) and personal observations between 1st September, 2013 and 2nd December, 2013. This study covered key characteristics of goats production and areas of goat breeding, such as general farm details, number of goats, main activities of the farmers, farming types, breeds, flock structure, feeding, housing, catastrophes, selection, mating systems, breeding systems, average age at parturition, breeding problems, and the importance of goats. Results showed that the average number of goats in Kajiado was 100.65 ± std 49.88 while in Makueni it was 12.28 ± std 6.46. The main activity of the people interviewed was farming as 58 people (96.67%) in Kajiado and 42 people (61.60%) chose farming as their main activity because this was their main source of livelihood. Flocks were dominated by breeding females at a mean of 39.06 ± std 16.75 in Kajiado and a mean of 5.62 ± std 3.50 in Makueni because females were kept to reproduce to increase the size of the flock and the males were kept majorly for cash and only one or two were left to reproduce with the females. Drought was the major catastrophe as it killed an average number of goats of 6.33 ± std 4.36. Pneumonia and diarrhoea were the major diseases according to 28 farmers (46.66%) in Kajiado and 31 farmers (51.66%) in Makueni. Ticks and fleas were the major parasites according to 42 farmers (70%) in Kajiado and 4 farmers (63.34%) in Makueni. Treatment was mostly done by the farmers individually as 54 farmers (90%) in Kajiado and 46 farmers (76.67%) in Makueni treated the animals by themselves. This was so because it was either not easy to get a veterinarian or expensive for them to hire veterinarian doctors. Some farmers used traditional medicine like mavuavui;Steganotaenia araliacea was used to treat pneumonia. Farmers also devised feeding methods during drought as 48 farmers (80.00%) in Kajiado and 23 farmers (38.33%) in Makueni cut leaves from up trees to feed the goats. When doing selection of breed, 58 farmers (96.67%) and 57 farmers (95%) considered large body size and drought resistance respectively in Kajiado. The farmers in Makueni considered age and drought resistance at equal chances of 59 farmers (98.33%). The main mating system was naturally uncontrolled as 113 farmers (95.17%) of the overall 120 farmers interviewed in Kajiado and Makueni chose this as the main mating method. The major breeding system was pure breeding at 85 farmers (70.83%). The average age at parturition of the goats was 1.435 ± 0.125 years in Kajiado and 1.44 ± 0.121 years in Makueni. Abortion was the major breeding problem because it was caused by environmental stressors like drought and diseases as 54 farmers (93.92%) in Kajiado and 55 farmers (95.66%) claimed that it was a problem. Goats were majorly kept for cash (100%) and meat (100%) in Kajiado and for cash (100%) and dowry (100%) in Makueni. In conclusion, the study showed that crossbreeding was encouraged by mating that occurred at the markets, water points, free ranging feeding method, pastoralism due to drought, selection methods and translocation of female goats from Makueni County to be mated with the males in Kajiado County. Environmental problems like drought and diseases caused several deaths and reduced the level of existing gene pool of the goats.
