After being degreased with acetone, the feInal gonad of Chlamys (Azumapecten)farreri was extracted with watr, and then subjected to DEAE - cellulose column chromatogrphyy and pu-rified by Sephadex G - 100 chromatograp...After being degreased with acetone, the feInal gonad of Chlamys (Azumapecten)farreri was extracted with watr, and then subjected to DEAE - cellulose column chromatogrphyy and pu-rified by Sephadex G - 100 chromatography to obain a pure female gonad glycoprotein (FGGP). The IRspectra, electrophoresis and chemical analysis of FGGP indicated tha it was a kind of acid glycoproteinand that the content of total protein and sugar were 54. 8% and 40%, respectively. Pharmacological testsshawd that FGGP could inhibit (inhibition rate of 40. 87% ) the growth of Sarorna 180 of mice.展开更多
The glutathione S-transferases(GSTs)are a superfamily of enzymes that function in cellular protection against toxic substances and oxidative stress.Bivalves could accumulate high concentration of paralytic shellfish t...The glutathione S-transferases(GSTs)are a superfamily of enzymes that function in cellular protection against toxic substances and oxidative stress.Bivalves could accumulate high concentration of paralytic shellfish toxins(PSTs)from harmful algae.To understand the possible involvement of GSTs in protecting bivalves during PST accumulation and metabolism,the GST genes were systemically analyzed in two cultured scallops,Azumapecten farreri and Mizuhopecten yessoensis,which were reported for PST deposition during harmful algae bloom.A total of 35 and 37 GSTs were identified in A.farreri(AfGSTs)and M.yessoensis(MyGSTs)genome,respectively,and the expansion of the sigma class from the cytosolic subfamily was observed.In both scallop species,sigma class GSTs showed higher expression than other members.The high GSTs expression was detected mainly during/after larvae stages and in the two most toxic organs,hepatopancreas and kidney.After ingesting PST-producing dinoflagellates,all the regulated AfGSTs in the hepatopancrcas were from the sigma class,but with opposite regulation pattern between Alexandrium catenella and A.minutum exposure.In scallop kidneys,where PSTs transformed into higher toxicity,more AfGSTs were regulated than in the hepatopancreas,and most of them were from the sigma class,with similar regulation pattern between A.catenella and A.minutum exposure.In M.yessoensis exposed to A.catenella,MyGST-σ2 was the only up-regulated MyGST in both hepatopancreas and kidney.Our results suggested the possible diverse function of scallop GSTs and the importance of sigma class in the defense against PSTs,which would contribute to the adaptive evolution of scallops in marine environments.展开更多
文摘After being degreased with acetone, the feInal gonad of Chlamys (Azumapecten)farreri was extracted with watr, and then subjected to DEAE - cellulose column chromatogrphyy and pu-rified by Sephadex G - 100 chromatography to obain a pure female gonad glycoprotein (FGGP). The IRspectra, electrophoresis and chemical analysis of FGGP indicated tha it was a kind of acid glycoproteinand that the content of total protein and sugar were 54. 8% and 40%, respectively. Pharmacological testsshawd that FGGP could inhibit (inhibition rate of 40. 87% ) the growth of Sarorna 180 of mice.
基金This work was funded by the National Key R&D Program of China(2019YFC1605704)the National Natural Science Foundation of China(31630081 and 31802292)Taishan Industry Leading Talent Project.
文摘The glutathione S-transferases(GSTs)are a superfamily of enzymes that function in cellular protection against toxic substances and oxidative stress.Bivalves could accumulate high concentration of paralytic shellfish toxins(PSTs)from harmful algae.To understand the possible involvement of GSTs in protecting bivalves during PST accumulation and metabolism,the GST genes were systemically analyzed in two cultured scallops,Azumapecten farreri and Mizuhopecten yessoensis,which were reported for PST deposition during harmful algae bloom.A total of 35 and 37 GSTs were identified in A.farreri(AfGSTs)and M.yessoensis(MyGSTs)genome,respectively,and the expansion of the sigma class from the cytosolic subfamily was observed.In both scallop species,sigma class GSTs showed higher expression than other members.The high GSTs expression was detected mainly during/after larvae stages and in the two most toxic organs,hepatopancreas and kidney.After ingesting PST-producing dinoflagellates,all the regulated AfGSTs in the hepatopancrcas were from the sigma class,but with opposite regulation pattern between Alexandrium catenella and A.minutum exposure.In scallop kidneys,where PSTs transformed into higher toxicity,more AfGSTs were regulated than in the hepatopancreas,and most of them were from the sigma class,with similar regulation pattern between A.catenella and A.minutum exposure.In M.yessoensis exposed to A.catenella,MyGST-σ2 was the only up-regulated MyGST in both hepatopancreas and kidney.Our results suggested the possible diverse function of scallop GSTs and the importance of sigma class in the defense against PSTs,which would contribute to the adaptive evolution of scallops in marine environments.
