Compounds isolated from Epimedium include the total flavonoids of Epimedium,icariin,and its metabolites(icaritin,icariside Ⅰ,and icariside Ⅱ),which have similar molecular structures.Modern pharmacological research a...Compounds isolated from Epimedium include the total flavonoids of Epimedium,icariin,and its metabolites(icaritin,icariside Ⅰ,and icariside Ⅱ),which have similar molecular structures.Modern pharmacological research and clinical practice have proved that Epimedium and its active components have a wide range of pharmacological effects,especially in improving sexual function,hormone regulation,anti-osteoporosis,immune function regulation,anti-oxidation,and anti-tumor activity.To date,we still need a comprehensive source of knowledge about the pharmacological effects of Epimedium and its bioactive compounds on the male reproductive system.However,their actions in other tissues have been reviewed in recent years.This review critically focuses on the Epimedium,its bioactive compounds,and the biochemical and molecular mechanisms that modulate vital pathways associated with the male reproductive system.Such intrinsic knowledge will significantly further studies on the Epimedium and its bioactive compounds that protect the male reproductive system and provide some guidances for clinical treatment of related male reproductive disorders.展开更多
[Objectives]Epimedium brevicornu Maxim is a well-known plant having the concomitant function of both medicine and foodstuff with the bioactivity of anti-inflammatory and cardiovascular protection.It has important ther...[Objectives]Epimedium brevicornu Maxim is a well-known plant having the concomitant function of both medicine and foodstuff with the bioactivity of anti-inflammatory and cardiovascular protection.It has important therapeutic effects and health benefits for various chronic diseases.This study aimed to analyze the studies on E.brevicornu Maxim from 2003 to 2022 with bibliometric analysis.[Methods]Publications related to E.brevicornu Maxim was retrieved in Web of Science Core Collection(WoSCC)from January 1,2003 to November 19,2022,and analyzed in Microsoft Excle,CiteSpace,and VOSviewer.[Results]In total,1317 documents were extracted from the WoSCC database.The growth rate of the publications showed a rapid increase from 2017.China provided the most documents,and Chinese Academy of Medical Sciences,Fudan University and Chinese Academy of Sciences contributed more papers.Wang Ying from Chinese Academy of Sciences was the most productive author,and the Journal of Ethnopharmacology was the most co-cited journal.The words"icariin","activation","oxidative stress","apoptosis","proliferation","osteogenic differentiation"and"flavonoid"were frequently occurred in the abstract and title of articles.Cluster analysis of keywords demonstrated"hippocampal neuron","efficient production","liquid extraction","bone regeneration"and"leukemia cell growth"were the hot topics from 2003 to 2022.The focus of the research has changed from the liquid extraction of flavonoids to the inflammatory response and metabolism,while cognitive deficit has emerged as a recent research hotspot.[Conclusions]The research on E.brevicornu Maxim has been expanding,and more studies are related to signaling pathways and metabolism diseases.展开更多
This study investigates the diurnal patterns of nectar secretion,sugar content,and caloric value in Epimedium wushanense,and their interaction mechanisms with pollinator behavior under varying environmental conditions...This study investigates the diurnal patterns of nectar secretion,sugar content,and caloric value in Epimedium wushanense,and their interaction mechanisms with pollinator behavior under varying environmental conditions.Nectar secretion exhibited a diurnal pattern,peaking between 11:00 and 13:00,with progressive increases in both volumes(19.07±1.66μL/day)and caloric value(6.03±0.55 cal/day)over four consecutive days,culminating in maximal production on Day 4(p<0.05).Floral bagging significantly altered nectar traits(Mann-Whitney U test,p<0.05),with bagged inflorescences demonstrating 61.82%higher nectar volume productivity relative to unbagged controls.Pollinator visitation,primarily by Bombus trifasciatus and Bombus grahami,was strongly correlated with nectar sugar concentration and distribution,peaking during midday when temperatures and humidity were optimal.Notably,B.trifasciatus displayed legitimate pollination behavior,while B.grahami exhibited nectar robbing.Bombus grahami peaked at 15:00(7.67±0.33 visits)under 22.8°C/58%RH,outperforming Bombus trifasciatus(5.67±0.27 at 13:00;p<0.05),highlighting differential pollinator effectiveness.Temperature negatively impacted unbagged nectar volume and caloric value but positively influenced bagged nectar,suggesting adaptive resource allocation strategies.These findings underscore the intricate relationship between environmental factors,nectar dynamics,and pollinator behavior,revealing how E.wushanense optimizes reproductive success through temporal and ecological adaptations.This study provides critical insights into the ecological mechanisms driving plant-pollinator interactions and resource allocation in changing environments.展开更多
To assess the potential effect of total flavonoids of epimedium （TFE） on cytochrome P450 and activity of its main isoforms in rat liver microsomes. TFE （300 mg/kg） was administered once daily to male Sprague-Dowle...To assess the potential effect of total flavonoids of epimedium （TFE） on cytochrome P450 and activity of its main isoforms in rat liver microsomes. TFE （300 mg/kg） was administered once daily to male Sprague-Dowley rats by gavage for fifteen days. The total cytochrome P450 content and its main isoforms CYP1A2, CYP3A4 and CYP2E1 activities in rat liver microsomes were detected. The activity of CYP1A2 was measured by fluorometry and the activities of CYP3A4 and CYP2E1 were determined by measuring the amount of methanal and p-aminophenol formed using UV/Vis spectrophotometer, respectively. Administration of TFE significantly increased the total CYP450 content and activities ofCYP1A2, CYP3A4 and CYP2E1 in rat liver microsomes, compared with the control group. Particularly, the activities of CYP1A2 and CYP2E1 were enhanced significantly （P〈0.01）. TFE induced the increase in total CYP450 content and its main isoforms CYP1A2, CYP3A4 and CYP2E1 activities in rat liver microsomes.展开更多
In the present paper is reported the method for the isolation and extraction of total flavonoids of Epimedium Koreanum Nakai by means of supercritical fluid extraction(SFE). By examining pressure, temperature, amounts...In the present paper is reported the method for the isolation and extraction of total flavonoids of Epimedium Koreanum Nakai by means of supercritical fluid extraction(SFE). By examining pressure, temperature, amounts of modifier and extraction time, the optimized condition of SFE is confirmed as 30 MPa and 60 ℃, with 70% ethanol as the modifier. The samples were statically extracted for 30 min, followed by dynamic extraction for 120 min at a flow rate of 6 mL/min. The quantitative analysis of total flavonoids was performed by UV-Vis spectrophotometry. Compared with the conventional method, the SFE method is more efficient, more rapid and more friendly environmentally.展开更多
Obesity is associated with a number of metabolic abnormalities such as type 2 diabetes and has become a major health problem worldwide. In the present study, we investigated the effects of Epimedium koreanum Nakai(Her...Obesity is associated with a number of metabolic abnormalities such as type 2 diabetes and has become a major health problem worldwide. In the present study, we investigated the effects of Epimedium koreanum Nakai(Herba Epimedii, HE) and its main constituent icariin on the adipocyte differentiation in 3T3-L1 preadipocytes. HE extract and icariin significantly reduced lipid accumulation and suppressed the expressions of PPARγ, C/EBPα, and SREBP-1c in 3T3-L1 adipocytes. They also inhibited fatty acid synthase(FAS), acyl-Co A synthase(ACS1), and perilipin. Moreover, HE extract and icariin markedly increased the phosphorylation of AMPK. These results indicated that HE extract and icariin can inhibit the adipocyte differentiation through downregulation of the adipogenic transcription factors, suggesting that HE containing icariin may be used as a potential therapeutic agent in the treatment and prevention of obesity.展开更多
In this paper, a sensitive and specific liquid chromatography-electrospray ionisation-mass spectrometry （LC-ESI-MS） method has been developed and validated for the identification and determination of seven flavonoid...In this paper, a sensitive and specific liquid chromatography-electrospray ionisation-mass spectrometry （LC-ESI-MS） method has been developed and validated for the identification and determination of seven flavonoids, namely epimedin A, epimedin B, epimedin C, icariin, sagittatoside B, 2＂-O-rhamnosyl icariside II, and baohuoside I in Epimedium from different sources.展开更多
Background Growth-promoting antibiotics have been banned by law in the livestock and poultry breeding industry in many countries.Various alternatives to antibiotics have been investigated for using in livestock.Epimed...Background Growth-promoting antibiotics have been banned by law in the livestock and poultry breeding industry in many countries.Various alternatives to antibiotics have been investigated for using in livestock.Epimedium(EM)is an herb rich in flavonoids that has many beneficial effects on animals.Therefore,this study was planned to explore the potential of EM as a new alternative antibiotic product in animal feed.Methods A total of 7201-day-old male broilers(Arbor Acres Plus)were randomly divided into six groups and fed basal diet(normal control;NC),basal diet supplemented with antibiotic(75 mg/kg chlortetracycline;CTC),and basal diet supplemented with 100,200,400 or 800 mg/kg EM extract for 6 weeks(EM100,EM200,EM400 and EM800 groups).The growth performance at weeks 3 and 6 was measured.Serum,intestinal tissue and feces were collected to assay for antioxidant indexes,intestinal permeability,lactic acid and short-chain fatty acids(SCFAs)profiles,microbial composition,and expression of intestinal barrier genes.Results The average daily feed intake in CTC group at 1-21 d was significantly higher than that in the NC group,and had no statistical difference with EM groups.Compared with NC group,average daily gain in CTC and EM200 groups increased significantly at 1-21 and 1-42 d.Compared with NC group,EM200 and EM400 groups had significantly decreased levels of lipopolysaccharide and D-lactic acid in serum throughout the study.The concentrations of lactic acid,acetic acid,propionic acid,butyric acid and SCFAs in feces of birds fed 200 mg/kg EM diet were significantly higher than those fed chlortetracycline.The dietary supplementation of chlortetracycline and 200 mg/kg EM significantly increased ileal expression of SOD1,Claudin-1 and ZO-1 genes.Dietary supplemented with 200 mg/kg EM increased the relative abundances of g_NK4A214_group and Lactobacillus in the jejunal,while the relative abundances of Microbacterium,Kitasatospora,Bacteroides in the jejunal and Gallibacterium in the ileum decreased.Conclusion Supplementation with 200 mg/kg EM extract improved the composition of intestinal microbiota by regulating the core bacterial genus Lactobacillus,and increased the concentration of beneficial metabolites lactic acid and SCFAs in the flora,thereby improving the antioxidant capacity and intestinal permeability,enhancing the function of tight junction proteins.These beneficial effects improved the growth performance of broilers.展开更多
A new aryltetrahydronaphthalene lignan, （＋）-cycloolivil formaldehyde condensate （1）, was isolated from the whole plants of Epimedium brevicornum （Berberidaceae）. Its structure was elucidated on the basis of MS,...A new aryltetrahydronaphthalene lignan, （＋）-cycloolivil formaldehyde condensate （1）, was isolated from the whole plants of Epimedium brevicornum （Berberidaceae）. Its structure was elucidated on the basis of MS, 1D and 2D NMR techniques.展开更多
Six new prenylated flavonoid glycosides,including four new furan-flavonoid glycosides wushepimedoside A–D(1–4)and two new prenyl flavonoid derivatives wushepimedoside E–F(5–6),and one know analog epimedkoreside B(...Six new prenylated flavonoid glycosides,including four new furan-flavonoid glycosides wushepimedoside A–D(1–4)and two new prenyl flavonoid derivatives wushepimedoside E–F(5–6),and one know analog epimedkoreside B(7)were isolated from biotransformation products of the aerial parts of Epimedium wushanense.Their structures were elucidated according to comprehensive analysis of HR-MS and NMR spectroscopic data,and the absolute configurations were assigned using experimental and calculated electronic circular dichroism(ECD)data.The regulatory activity of compounds 1–7 on the production of testosterone in primary rat Leydig cells were investigated,and 4 and 5 exhibited testosterone production-promoting activities.Molecular docking analysis suggested that bioactive compounds 4 and 5 showed the stable binding with 3β-HSD and 4 also had good affinity with Cyp17A1,which suggested that these compounds may regulate testosterone production through stimulating the expression of the above two key proteins.展开更多
The effect of Epimedium extract （EE） on erythrocytes was investigated by atomic force microscopy （AFM）. The images of the surface structures showed clear concave and progressive increase of surface roughness of er...The effect of Epimedium extract （EE） on erythrocytes was investigated by atomic force microscopy （AFM）. The images of the surface structures showed clear concave and progressive increase of surface roughness of erythrocyte after incubation with EE at concentration of 0.2 or 0.05 g/L, far below its critical hemolytic levels. The AFM results also indicated that the granules of the fine surface structure increased, which caused by aggregation of membrane protein. Further study showed that the change in surface topography of erythrocyte membrane might be connected with the increase of intracellular free Ca^2＋ induced by EE.展开更多
Three Epimedium species,E.pubescens Maxim.,E.sagittatum(Sieb.&Zucc.)Maxim.,and E.wushanense T.S.Ying,which are sympatrically distributed in the western Hubei Province,have been used in traditional Chinese medicine...Three Epimedium species,E.pubescens Maxim.,E.sagittatum(Sieb.&Zucc.)Maxim.,and E.wushanense T.S.Ying,which are sympatrically distributed in the western Hubei Province,have been used in traditional Chinese medicine(TCM)for about 2,000 years.Genetic variability and population genetic structure of 11 natural populations of these Epimedium species were investigated using isoelectric focusing in thin-layer polyacrylamide slab gels.Of the 22 enzyme systems prescreened,six coding for 13 loci and 45 alleles were resolved,which were used for analyzing genetic diversity and population structure at both intraspecific and interspecific levels.The results showed that:l)high levels of genetic diversity were observed in all three species(A=2.6-3.2,P=69.2%-84.6%,Ho=0.274-0.377,HE=0.282-0.369),which were higher than that of other herbaceous and aulmal-pollinated species with similar life-history characteristics;2)there was significant deviation from Hardy-Weinberg Equilibrium,with one half of the loci showing heterozygote excess and the other homozygote excess,in all populations,suggesting the complicated breeding system of Epimedium species;3)the low level of intraspecific and interspecific genetic differentiation(GST=0.0246-0.0409 and 0.0495-0.1213,respectively)indicated a high level of gene flow among populations and close genetic relationship among the three species;and 4)UPGMA cluster analysis further showed that E.pubescens was more closely related to E.sagittatum than to E.wushanense,which was in good agreement with the morphological characters and the recent phylogenetic analysis of these species.On the basis of these results,it was concluded that the mixed breeding system,long-lived perennial life form,ancient evolutionary history,and seed dispersal by ants in Epimedium are responsible for the genetic variation and population structure of these species.展开更多
Four new prenylflavonoid glycosides,namely koreanoside H-K(1-4),together with eleven known ones(5-15)were isolated from the leaves of Epimedium koreanum Nakai.Their structures were elucidated by ID NMR,2D NMR,HR-ESI-M...Four new prenylflavonoid glycosides,namely koreanoside H-K(1-4),together with eleven known ones(5-15)were isolated from the leaves of Epimedium koreanum Nakai.Their structures were elucidated by ID NMR,2D NMR,HR-ESI-MS,IR and UV.The identification of the sugar moieties was carried out by means of acid hydrolysis and HPLC analysis of their derivatives.It is worth noting that compound 3 and compound 4 were elucidated to contain fucose and quinovose moieties,which were two extremely rare sugar units from the genus Epimedium.The anti-pulmonary fibrosis activity of the new compounds was evaluated using A549 cell line.Compounds 1,2 and 4 showed significant anti-pulmonary fibrosis activities.展开更多
A novel flavone glycoside named 3＇＇＇-carbonyl-2＇＇-β-L-quinovosyl icariside Ⅱ was isolated from the aerial parts of Epimedium koreanum. Its chemical structure was elucidated on the basis of spectral analysis.
Two new prenylflavonoids, named sutchuenmedin A （1） and sutchuenmedin B （2）, were isolated from the 70% EtOH extract of Epimedium sutchuenense. Their structures were determined from spectral analysis. C 2009 Guang...Two new prenylflavonoids, named sutchuenmedin A （1） and sutchuenmedin B （2）, were isolated from the 70% EtOH extract of Epimedium sutchuenense. Their structures were determined from spectral analysis. C 2009 Guang Chen. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved,展开更多
Aim To develop a rapid, effective method for the determination of flavonoidsand ginsenosides in one injection and evaluate the flavonoids and ginsenosides content to controlthe ratio of Epimedium and Ginseng herbs in ...Aim To develop a rapid, effective method for the determination of flavonoidsand ginsenosides in one injection and evaluate the flavonoids and ginsenosides content to controlthe ratio of Epimedium and Ginseng herbs in botanical combinations. Methods The quality evaluationwas determinatted using reversed-phase high performance liquid chromatog-raphy (HPLC), referred bythe major flavonoids from Epimedium, epimedin A, epimedin B, epimedin C, and icariin as thestandards, and the major ginsenosides Rg_1, Re, Rf, Rb_1, Rb_2, and Rd as the standards, includedEpimedium brevicornum Maxim., E. sagittatum (Sieb. et Zucc) Maxim., E. koreanum NaKai, P. ginseng C.A. Meyer, P. quinquefolium L., P. notoginseng and some products containing the above herbs. ResultsThe main flavonoids and ginsenosides could be clearly resolved in the single analysis. ConclusionThe results can be effectively used in evaluating qualitatively and quantitatively the ration ofEpimedium and Ginseng contained products.展开更多
基金supported by the National Nature Science Foundation of China(NSFC,No.31960156,No.31660338,No.31760627,and No.32270848)Collaborative Innovation Center of Chinese Ministry of Education(2020-39),Science and Technology Support Program of Guizhou Province(QKH[2021]111,QKH[2020]4Y192,QKH[2018]5772-006,and QKH[2019]5406)Science and Technology Fund of Guizhou Provincial Health Commission(gzwkj2022-019).
文摘Compounds isolated from Epimedium include the total flavonoids of Epimedium,icariin,and its metabolites(icaritin,icariside Ⅰ,and icariside Ⅱ),which have similar molecular structures.Modern pharmacological research and clinical practice have proved that Epimedium and its active components have a wide range of pharmacological effects,especially in improving sexual function,hormone regulation,anti-osteoporosis,immune function regulation,anti-oxidation,and anti-tumor activity.To date,we still need a comprehensive source of knowledge about the pharmacological effects of Epimedium and its bioactive compounds on the male reproductive system.However,their actions in other tissues have been reviewed in recent years.This review critically focuses on the Epimedium,its bioactive compounds,and the biochemical and molecular mechanisms that modulate vital pathways associated with the male reproductive system.Such intrinsic knowledge will significantly further studies on the Epimedium and its bioactive compounds that protect the male reproductive system and provide some guidances for clinical treatment of related male reproductive disorders.
基金Supported by The Scientific Research Program(B2021234)from Hubei Provincial Department of EducationKey Research and Development Program of Hubei Province(2022BCE013)+2 种基金Open fund of Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization(202303302)Scientific Research Foundation for Advanced Talents(2042021040)from Huanggang Normal UniversityChinese Foundation for Hepatitis Prevention and Control-Chinese Hepatitis B Research Fund(YGFK20220019).
文摘[Objectives]Epimedium brevicornu Maxim is a well-known plant having the concomitant function of both medicine and foodstuff with the bioactivity of anti-inflammatory and cardiovascular protection.It has important therapeutic effects and health benefits for various chronic diseases.This study aimed to analyze the studies on E.brevicornu Maxim from 2003 to 2022 with bibliometric analysis.[Methods]Publications related to E.brevicornu Maxim was retrieved in Web of Science Core Collection(WoSCC)from January 1,2003 to November 19,2022,and analyzed in Microsoft Excle,CiteSpace,and VOSviewer.[Results]In total,1317 documents were extracted from the WoSCC database.The growth rate of the publications showed a rapid increase from 2017.China provided the most documents,and Chinese Academy of Medical Sciences,Fudan University and Chinese Academy of Sciences contributed more papers.Wang Ying from Chinese Academy of Sciences was the most productive author,and the Journal of Ethnopharmacology was the most co-cited journal.The words"icariin","activation","oxidative stress","apoptosis","proliferation","osteogenic differentiation"and"flavonoid"were frequently occurred in the abstract and title of articles.Cluster analysis of keywords demonstrated"hippocampal neuron","efficient production","liquid extraction","bone regeneration"and"leukemia cell growth"were the hot topics from 2003 to 2022.The focus of the research has changed from the liquid extraction of flavonoids to the inflammatory response and metabolism,while cognitive deficit has emerged as a recent research hotspot.[Conclusions]The research on E.brevicornu Maxim has been expanding,and more studies are related to signaling pathways and metabolism diseases.
基金supported by Sichuan Science and Technology Program(No.2023NSFSC1282)Talent Foundation of China West Normal University(Nos.17YC142 and 17YC137)+1 种基金the National General Cultivation Project of China West Normal University(No.19B029)Youth Foundation Specialization of West China Normal University(No.22KB004).
文摘This study investigates the diurnal patterns of nectar secretion,sugar content,and caloric value in Epimedium wushanense,and their interaction mechanisms with pollinator behavior under varying environmental conditions.Nectar secretion exhibited a diurnal pattern,peaking between 11:00 and 13:00,with progressive increases in both volumes(19.07±1.66μL/day)and caloric value(6.03±0.55 cal/day)over four consecutive days,culminating in maximal production on Day 4(p<0.05).Floral bagging significantly altered nectar traits(Mann-Whitney U test,p<0.05),with bagged inflorescences demonstrating 61.82%higher nectar volume productivity relative to unbagged controls.Pollinator visitation,primarily by Bombus trifasciatus and Bombus grahami,was strongly correlated with nectar sugar concentration and distribution,peaking during midday when temperatures and humidity were optimal.Notably,B.trifasciatus displayed legitimate pollination behavior,while B.grahami exhibited nectar robbing.Bombus grahami peaked at 15:00(7.67±0.33 visits)under 22.8°C/58%RH,outperforming Bombus trifasciatus(5.67±0.27 at 13:00;p<0.05),highlighting differential pollinator effectiveness.Temperature negatively impacted unbagged nectar volume and caloric value but positively influenced bagged nectar,suggesting adaptive resource allocation strategies.These findings underscore the intricate relationship between environmental factors,nectar dynamics,and pollinator behavior,revealing how E.wushanense optimizes reproductive success through temporal and ecological adaptations.This study provides critical insights into the ecological mechanisms driving plant-pollinator interactions and resource allocation in changing environments.
基金Scientific Research Foundation of Shanghai Traditional Chinese Medicine in China(Grant No.2004J009C).
文摘To assess the potential effect of total flavonoids of epimedium （TFE） on cytochrome P450 and activity of its main isoforms in rat liver microsomes. TFE （300 mg/kg） was administered once daily to male Sprague-Dowley rats by gavage for fifteen days. The total cytochrome P450 content and its main isoforms CYP1A2, CYP3A4 and CYP2E1 activities in rat liver microsomes were detected. The activity of CYP1A2 was measured by fluorometry and the activities of CYP3A4 and CYP2E1 were determined by measuring the amount of methanal and p-aminophenol formed using UV/Vis spectrophotometer, respectively. Administration of TFE significantly increased the total CYP450 content and activities ofCYP1A2, CYP3A4 and CYP2E1 in rat liver microsomes, compared with the control group. Particularly, the activities of CYP1A2 and CYP2E1 were enhanced significantly （P〈0.01）. TFE induced the increase in total CYP450 content and its main isoforms CYP1A2, CYP3A4 and CYP2E1 activities in rat liver microsomes.
基金the Natural Science and Technology Foundation of Jilin Province(No.2 0 0 2 0 6 37- 1)
文摘In the present paper is reported the method for the isolation and extraction of total flavonoids of Epimedium Koreanum Nakai by means of supercritical fluid extraction(SFE). By examining pressure, temperature, amounts of modifier and extraction time, the optimized condition of SFE is confirmed as 30 MPa and 60 ℃, with 70% ethanol as the modifier. The samples were statically extracted for 30 min, followed by dynamic extraction for 120 min at a flow rate of 6 mL/min. The quantitative analysis of total flavonoids was performed by UV-Vis spectrophotometry. Compared with the conventional method, the SFE method is more efficient, more rapid and more friendly environmentally.
基金supported by Basic Science Research Program through the National Research Foundation of Korea(NRF)funded by the Ministry of Science,ICT,&Future Planning(No.2014R1A1A1003758)
文摘Obesity is associated with a number of metabolic abnormalities such as type 2 diabetes and has become a major health problem worldwide. In the present study, we investigated the effects of Epimedium koreanum Nakai(Herba Epimedii, HE) and its main constituent icariin on the adipocyte differentiation in 3T3-L1 preadipocytes. HE extract and icariin significantly reduced lipid accumulation and suppressed the expressions of PPARγ, C/EBPα, and SREBP-1c in 3T3-L1 adipocytes. They also inhibited fatty acid synthase(FAS), acyl-Co A synthase(ACS1), and perilipin. Moreover, HE extract and icariin markedly increased the phosphorylation of AMPK. These results indicated that HE extract and icariin can inhibit the adipocyte differentiation through downregulation of the adipogenic transcription factors, suggesting that HE containing icariin may be used as a potential therapeutic agent in the treatment and prevention of obesity.
文摘In this paper, a sensitive and specific liquid chromatography-electrospray ionisation-mass spectrometry （LC-ESI-MS） method has been developed and validated for the identification and determination of seven flavonoids, namely epimedin A, epimedin B, epimedin C, icariin, sagittatoside B, 2＂-O-rhamnosyl icariside II, and baohuoside I in Epimedium from different sources.
基金supported by The National Key Research and Development Program of China(2021YFD1300301/2021YFD1300303)The Special Basic Research Fund for Central Public Research Institutes(2021-YWF-ZYSQ-06)+1 种基金The Agricultural Science and Technology Innovation Program(ASTIP)in CAAS and its key projects(CAAS-ZDRW202111)The Chinese Academy of Agricultural Science and Technology Innovation Project(ASTIP-IAS-12)。
文摘Background Growth-promoting antibiotics have been banned by law in the livestock and poultry breeding industry in many countries.Various alternatives to antibiotics have been investigated for using in livestock.Epimedium(EM)is an herb rich in flavonoids that has many beneficial effects on animals.Therefore,this study was planned to explore the potential of EM as a new alternative antibiotic product in animal feed.Methods A total of 7201-day-old male broilers(Arbor Acres Plus)were randomly divided into six groups and fed basal diet(normal control;NC),basal diet supplemented with antibiotic(75 mg/kg chlortetracycline;CTC),and basal diet supplemented with 100,200,400 or 800 mg/kg EM extract for 6 weeks(EM100,EM200,EM400 and EM800 groups).The growth performance at weeks 3 and 6 was measured.Serum,intestinal tissue and feces were collected to assay for antioxidant indexes,intestinal permeability,lactic acid and short-chain fatty acids(SCFAs)profiles,microbial composition,and expression of intestinal barrier genes.Results The average daily feed intake in CTC group at 1-21 d was significantly higher than that in the NC group,and had no statistical difference with EM groups.Compared with NC group,average daily gain in CTC and EM200 groups increased significantly at 1-21 and 1-42 d.Compared with NC group,EM200 and EM400 groups had significantly decreased levels of lipopolysaccharide and D-lactic acid in serum throughout the study.The concentrations of lactic acid,acetic acid,propionic acid,butyric acid and SCFAs in feces of birds fed 200 mg/kg EM diet were significantly higher than those fed chlortetracycline.The dietary supplementation of chlortetracycline and 200 mg/kg EM significantly increased ileal expression of SOD1,Claudin-1 and ZO-1 genes.Dietary supplemented with 200 mg/kg EM increased the relative abundances of g_NK4A214_group and Lactobacillus in the jejunal,while the relative abundances of Microbacterium,Kitasatospora,Bacteroides in the jejunal and Gallibacterium in the ileum decreased.Conclusion Supplementation with 200 mg/kg EM extract improved the composition of intestinal microbiota by regulating the core bacterial genus Lactobacillus,and increased the concentration of beneficial metabolites lactic acid and SCFAs in the flora,thereby improving the antioxidant capacity and intestinal permeability,enhancing the function of tight junction proteins.These beneficial effects improved the growth performance of broilers.
基金financially supported by the Foundation for the Author of National Excellent Doctoral Dissertation of China(No.00780)Specialized Research Fund for the Doctoral Program of Higher Education(No.2009531411 0001)the Program for New Century Excellent Talents on University(No.NCET-06-0824)
文摘A new aryltetrahydronaphthalene lignan, （＋）-cycloolivil formaldehyde condensate （1）, was isolated from the whole plants of Epimedium brevicornum （Berberidaceae）. Its structure was elucidated on the basis of MS, 1D and 2D NMR techniques.
基金supported by the National Science and Technology Major Project(No.2017ZX09301072)China Postdoctoral Science Foundation(No.2016M603041).
文摘Six new prenylated flavonoid glycosides,including four new furan-flavonoid glycosides wushepimedoside A–D(1–4)and two new prenyl flavonoid derivatives wushepimedoside E–F(5–6),and one know analog epimedkoreside B(7)were isolated from biotransformation products of the aerial parts of Epimedium wushanense.Their structures were elucidated according to comprehensive analysis of HR-MS and NMR spectroscopic data,and the absolute configurations were assigned using experimental and calculated electronic circular dichroism(ECD)data.The regulatory activity of compounds 1–7 on the production of testosterone in primary rat Leydig cells were investigated,and 4 and 5 exhibited testosterone production-promoting activities.Molecular docking analysis suggested that bioactive compounds 4 and 5 showed the stable binding with 3β-HSD and 4 also had good affinity with Cyp17A1,which suggested that these compounds may regulate testosterone production through stimulating the expression of the above two key proteins.
文摘The effect of Epimedium extract （EE） on erythrocytes was investigated by atomic force microscopy （AFM）. The images of the surface structures showed clear concave and progressive increase of surface roughness of erythrocyte after incubation with EE at concentration of 0.2 or 0.05 g/L, far below its critical hemolytic levels. The AFM results also indicated that the granules of the fine surface structure increased, which caused by aggregation of membrane protein. Further study showed that the change in surface topography of erythrocyte membrane might be connected with the increase of intracellular free Ca^2＋ induced by EE.
基金This work was supported by the National Natural Science Foundation of China(No.30570171)100 Talents Program of Chinese Academy of Sciences(No.05045112)Wuhan Chenguang Project(No.20055003059-45).
文摘Three Epimedium species,E.pubescens Maxim.,E.sagittatum(Sieb.&Zucc.)Maxim.,and E.wushanense T.S.Ying,which are sympatrically distributed in the western Hubei Province,have been used in traditional Chinese medicine(TCM)for about 2,000 years.Genetic variability and population genetic structure of 11 natural populations of these Epimedium species were investigated using isoelectric focusing in thin-layer polyacrylamide slab gels.Of the 22 enzyme systems prescreened,six coding for 13 loci and 45 alleles were resolved,which were used for analyzing genetic diversity and population structure at both intraspecific and interspecific levels.The results showed that:l)high levels of genetic diversity were observed in all three species(A=2.6-3.2,P=69.2%-84.6%,Ho=0.274-0.377,HE=0.282-0.369),which were higher than that of other herbaceous and aulmal-pollinated species with similar life-history characteristics;2)there was significant deviation from Hardy-Weinberg Equilibrium,with one half of the loci showing heterozygote excess and the other homozygote excess,in all populations,suggesting the complicated breeding system of Epimedium species;3)the low level of intraspecific and interspecific genetic differentiation(GST=0.0246-0.0409 and 0.0495-0.1213,respectively)indicated a high level of gene flow among populations and close genetic relationship among the three species;and 4)UPGMA cluster analysis further showed that E.pubescens was more closely related to E.sagittatum than to E.wushanense,which was in good agreement with the morphological characters and the recent phylogenetic analysis of these species.On the basis of these results,it was concluded that the mixed breeding system,long-lived perennial life form,ancient evolutionary history,and seed dispersal by ants in Epimedium are responsible for the genetic variation and population structure of these species.
基金supported by the National Natural Science Foundation of China(Nos.81673548 and U20A20407)Liaoning Revitalization Talents Program(No.XLYC1902119).
文摘Four new prenylflavonoid glycosides,namely koreanoside H-K(1-4),together with eleven known ones(5-15)were isolated from the leaves of Epimedium koreanum Nakai.Their structures were elucidated by ID NMR,2D NMR,HR-ESI-MS,IR and UV.The identification of the sugar moieties was carried out by means of acid hydrolysis and HPLC analysis of their derivatives.It is worth noting that compound 3 and compound 4 were elucidated to contain fucose and quinovose moieties,which were two extremely rare sugar units from the genus Epimedium.The anti-pulmonary fibrosis activity of the new compounds was evaluated using A549 cell line.Compounds 1,2 and 4 showed significant anti-pulmonary fibrosis activities.
文摘A novel flavone glycoside named 3＇＇＇-carbonyl-2＇＇-β-L-quinovosyl icariside Ⅱ was isolated from the aerial parts of Epimedium koreanum. Its chemical structure was elucidated on the basis of spectral analysis.
基金supported by Young Teacher Grant from Beijing University of Chemical Technology(No.QN0615).
文摘Two new prenylflavonoids, named sutchuenmedin A （1） and sutchuenmedin B （2）, were isolated from the 70% EtOH extract of Epimedium sutchuenense. Their structures were determined from spectral analysis. C 2009 Guang Chen. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved,
文摘Aim To develop a rapid, effective method for the determination of flavonoidsand ginsenosides in one injection and evaluate the flavonoids and ginsenosides content to controlthe ratio of Epimedium and Ginseng herbs in botanical combinations. Methods The quality evaluationwas determinatted using reversed-phase high performance liquid chromatog-raphy (HPLC), referred bythe major flavonoids from Epimedium, epimedin A, epimedin B, epimedin C, and icariin as thestandards, and the major ginsenosides Rg_1, Re, Rf, Rb_1, Rb_2, and Rd as the standards, includedEpimedium brevicornum Maxim., E. sagittatum (Sieb. et Zucc) Maxim., E. koreanum NaKai, P. ginseng C.A. Meyer, P. quinquefolium L., P. notoginseng and some products containing the above herbs. ResultsThe main flavonoids and ginsenosides could be clearly resolved in the single analysis. ConclusionThe results can be effectively used in evaluating qualitatively and quantitatively the ration ofEpimedium and Ginseng contained products.
