The doubled haploid(DH)technique accelerates homozygosity by inducing chromosome doubling in haploid embryos derived from hybrid plants.This approach offers significant advantages over conventional rice breeding metho...The doubled haploid(DH)technique accelerates homozygosity by inducing chromosome doubling in haploid embryos derived from hybrid plants.This approach offers significant advantages over conventional rice breeding methods by shortening the breeding cycle and enabling rapid development of pure homozygous lines.Anther culture(AC)has been established as an efficient and successful method for producing DH plants via androgenesis in rice.However,despite its success in japonica rice.展开更多
The fertile soil of the countryside is also rich in artistic inspiration.“If farmers pick up a brush,they can become artists.If one devotes oneself wholeheartedly to something,a stroke of genius will follow,”said Mi...The fertile soil of the countryside is also rich in artistic inspiration.“If farmers pick up a brush,they can become artists.If one devotes oneself wholeheartedly to something,a stroke of genius will follow,”said Miao Huixin,a cultural envoy to Shengfeng Village in Youchegang Town,Xiuzhou District,Jiaxing City,Zhejiang Province.展开更多
This paper looks back to the development of plant tissue culture in China in the last century. Since 1934, tissue culture studies in China has kept up with the international development in the fields. Progress has bee...This paper looks back to the development of plant tissue culture in China in the last century. Since 1934, tissue culture studies in China has kept up with the international development in the fields. Progress has been made by Chinese in nearly every branches of tissue culture, including in vitro organogenesis, shoot tip culture, anther culture, ovary culture, endosperm culture, protoplast culture as well as mass cell culture. On the basis of reviewing the articles written by Chinese on plant tissue culture, the internationally recognized contributions are specially mentioned. The applications of plant tissue culture to agriculture and industry in China are also introduced.展开更多
At dawn in Wufu Village,in Dinghu District of Zhaoqing City,Guangdong Province,the newly restored Chaoyangli Cultural Retreat is already welcoming its first visitors.A visitor surnamed Chen,who has travelled from Guan...At dawn in Wufu Village,in Dinghu District of Zhaoqing City,Guangdong Province,the newly restored Chaoyangli Cultural Retreat is already welcoming its first visitors.A visitor surnamed Chen,who has travelled from Guangzhou with his child,stops in front of the Chaxi Academy to admire local intangible cultural heritage crafts.展开更多
This work examines media through a decolonial lens,centring Dagba$epistemologies to demonstrate how African languages,cultures,and traditions can transform how we think of knowledge.Focusing on film,television and rad...This work examines media through a decolonial lens,centring Dagba$epistemologies to demonstrate how African languages,cultures,and traditions can transform how we think of knowledge.Focusing on film,television and radio in Ghanaian African languages,the book argues for a decolonial politics and praxis in co-creating knowledge with Indigenous communities.It critically links the struggles of global majority countries,showing how colonialism and imperialism obstruct liberatory futures,while foregrounding African language media as vital tools for cultural and epistemological decolonisation.Heralded as part of an unstoppable shift away from Eurocentrism,the book is distinguished by its radical feminist subaltern analysis and its deep grounding in lived experience from Northern Ghana.By excavating local agency within the digital mediascape,Mohammed o!ers a crucial historical and political-economic theorisation of media practice and consumption.展开更多
In the year 2026,the world cultural exchanges will continue to develop,while the international public opinion environment remainsdive rseand complex.Looking back at this pivotal moment in history,the life journey of S...In the year 2026,the world cultural exchanges will continue to develop,while the international public opinion environment remainsdive rseand complex.Looking back at this pivotal moment in history,the life journey of Sunny Wu,Chairman of the Hong Kong Doyens’Club,resonates perfectly with the national development.展开更多
Probiotics can regulate gut microbes to maintain human health.However,the sensitivity of probiotics to environmental conditions reduces their bioavailability.In contrast,the formation of probiotic biofilm provides a n...Probiotics can regulate gut microbes to maintain human health.However,the sensitivity of probiotics to environmental conditions reduces their bioavailability.In contrast,the formation of probiotic biofilm provides a natural physical barrier against external interference.Our previous study established a dynamic culture system of the biofilm-state Bifidobacterium adolescentis Gr19(B-DC-B.adolescentis Gr19),forming higher density and more structurally stable biofilms,which enhanced its potential probiotic properties in vivo.Thus,the protective effect and mechanism of B-DC-B.adolescentis Gr19 on lipopolysaccharide(LPS)-induced intestinal barrier dysfunction were investigated in this study.The results showed that B-DC-B.adolescentis Gr19 not only had high resistance and adhesion activity,but also improved the intestinal barrier by increasing goblet cells and promoting the expression of tight junction(TJ)-related proteins.Moreover,B-DC-B.adolescentis Gr19 effectively attenuated intestinal barrier injury in Caco-2 cells by improving intestinal permeability and integrity.Remarkably,B-DC-B.adolescentis Gr19 enhanced expression of TJ proteins,restored localization of cytoskeleton and reduced intestinal inflammation by suppressing the Ras homolog family member A/Rho-associated coiled-coil-forming kinases/nuclear factor kappa B/myosin light chain kinase/myosin light chain(RhoA/ROCK/NF-κB/MLCK/MLC)pathway.Therefore,B-DC-B.adolescentis Gr19 plays a key role in mitigating LPS-induced intestinal barrier dysfunction.Overall,the present study provides a theoretical basis for ameliorating intestinal barrier dysfunction and developing novel functional foods by using biofilm-state probiotics under dynamic culture.展开更多
Background:Therapeutic responses of breast cancer vary among patients and lead to drug resistance and recurrence due to the heterogeneity.Current preclinical models,however,are inadequate for predicting individual pat...Background:Therapeutic responses of breast cancer vary among patients and lead to drug resistance and recurrence due to the heterogeneity.Current preclinical models,however,are inadequate for predicting individual patient responses towards different drugs.This study aimed to investigate the patient-derived breast cancer culture models for drug sensitivity evaluations.Methods:Tumor and adjacent tissues from female breast cancer patients were collected during surgery.Patient-derived breast cancer cells were cultured using the conditional reprogramming technique to establish 2D models.The obtained patient-derived conditional reprogramming breast cancer(CRBC)cells were subsequently embedded in alginate-gelatin methacryloyl hydrogel microspheres to form 3D culture models.Comparisons between 2D and 3D models were made using immunohistochemistry(tumor markers),MTS assays(cell viability),flow cytometry(apoptosis),transwell assays(migration),and Western blotting(protein expression).Drug sensitivity tests were conducted to evaluate patient-specific responses to anti-cancer agents.Results:2D and 3D culture models were successfully established using samples from eight patients.The 3D models retained histological and marker characteristics of the original tumors.Compared to 2D cultures,3D models exhibited increased apoptosis,enhanced drug resistance,elevated stem cell marker expression,and greater migration ability—features more reflective of in vivo tumor behavior.Conclusion:Patient-derived 3D CRBC models effectively mimic the in vivo tumor microenvironment and demonstrate stronger resistance to anti-cancer drugs than 2D models.These hydrogel-based models offer a cost-effective and clinically relevant platform for drug screening and personalized breast cancer treatment.展开更多
This paper undertakes a systematic combing of the development of research on integrating Chinese culture into foreign language education in China from the 1980s to 2025,dividing it into three stages:cultural attachmen...This paper undertakes a systematic combing of the development of research on integrating Chinese culture into foreign language education in China from the 1980s to 2025,dividing it into three stages:cultural attachment,cultural compensation,and cultural symbiosis,and reveals the logical shift of the research from the dominance of target language culture to the construction of the subjectivity of Chinese culture.Through quantitative and qualitative analysis of 435 CSSCI papers,three core themes are extracted:what to integrate,why to integrate,and how to integrate.This paper critically analyzes three pairs of contradictions:the imbalance between instrumentality and humanism,the separation of national narrative and individual expression,and the disconnection between traditional inheritance and modern transformation.It is proposed that future research should reconstruct the educational logic based on the Chinese context,integrate the national and individual dimensions,and build a dialogue mechanism between tradition and modernity,so as to provide theoretical and practical reference for the construction of a foreign language education system with Chinese characteristics.展开更多
BACKGROUND The unique survival environment of Helicobacter pylori(H.pylori)presents challenges in establishing appropriate in vitro culture conditions.The fastidious nature of H.pylori and its relatively low growth ra...BACKGROUND The unique survival environment of Helicobacter pylori(H.pylori)presents challenges in establishing appropriate in vitro culture conditions.The fastidious nature of H.pylori and its relatively low growth rate emphasize the need for customized culture conditions,which are essential for both research and clinical applications.AIM To identify the optimal liquid culture medium for H.pylori using both reference and clinical strains.METHODS Nine H.pylori strains were incubated in 10 different broth media commonly used for bacterial cultures under microaerophilic conditions.Bacterial concentrations were estimated using serial dilutions and the pour plate method.A range of H.pylori initial inoculum concentrations was tested for each strain.For growth evaluation,optical density at 600 nm was measured at 24,48,and 72 hours.The growth trends of H.pylori were compared among strains and media.RESULTS All H.pylori strains grew successfully over time,regardless of the initial inoculum concentration.The specific growth trends and the broth that yielded the highest optical density value at each time point varied with strain.The reference strains(H.pylori 26695,ATCC 43504,and J99)consistently exhibited robust growth,whereas the clinical strains displayed distinct media preferences.Overall,bacterial growth was higher in chopped meat carbohydrate broth,Columbia broth,and fastidious anaerobe broth than in Brucella broth after 72 hours.CONCLUSION Chopped meat carbohydrate broth was the most effective for H.pylori liquid culture,and Columbia broth and fastidious anaerobe broth also supported greater growth than Brucella broth.展开更多
Yunhe County in Lishui City,southwestern Zhejiang Province,doesn’t have any Disney castles.Instead,it has built bridges to the world with wood.Known as“China’s town of wooden toys,”accounting for about 40 percent ...Yunhe County in Lishui City,southwestern Zhejiang Province,doesn’t have any Disney castles.Instead,it has built bridges to the world with wood.Known as“China’s town of wooden toys,”accounting for about 40 percent of the global wooden toy market,Yunhe’s story began with a small factory established in 1970.Today,it has grown into a distinctive industrial cluster with an annual output value exceeding 8 billion yuan($1.15 billion),exporting products to 82 countries and regions.展开更多
Cell cultured meat has been extensively studied as an environmentally friendly,energy-saving and more effective technology.However,there are many technical bottlenecks,especially the regulatory mechanism and manufactu...Cell cultured meat has been extensively studied as an environmentally friendly,energy-saving and more effective technology.However,there are many technical bottlenecks,especially the regulatory mechanism and manufacturing method of in vitro myogenesis.Based on an edible modified silk protein scaffold,with 3D culturing,in situ differentiated and transcriptome analysis,this study describes novel scaffolds and fabrication methods for cell cultured meat.The results showed that the effective space and utilization efficiency for cell culture of the scaffold is 26–1000 that of the traditional culture dish;it could form a tissue-like structure.Transcriptomics revealed the regulatory pathways and key factors of different cycles.It clarifies that the multi-cycle process of myoblast myogenesis in vitro is different from the single feedback regulation in vivo.More importantly,a novel scaffold-based cell cultured meat manufacturing method was developed,further develop a new tissue culture solution that is different from existing cell culture meat production.For manufacturing processes,it provides a new cell culture meat technology system,provides a theoretical basis for the regulation of cell proliferation and muscle growth,and lays the technical foundation for in situ tissue culture of cell cultured meat in vitro.展开更多
Liposarcoma is one of the most common soft tissue sarcomas,however,its occurrence rate is still rare compared to other cancers.Due to its rarity,in vitro experiments are an essential approach to elucidate liposarcoma ...Liposarcoma is one of the most common soft tissue sarcomas,however,its occurrence rate is still rare compared to other cancers.Due to its rarity,in vitro experiments are an essential approach to elucidate liposarcoma pathobiology.Conventional cell culture-based research(2D cell culture)is still playing a pivotal role,while several shortcomings have been recently under discussion.In vivo,mouse models are usually adopted for pre-clinical analyses with expectations to overcome the issues of 2D cell culture.However,they do not fully recapitulate human dedifferentiated liposarcoma(DDLPS)characteristics.Therefore,three-dimensional(3D)culture systems have been the recent research focus in the cell biology field with the expectation to overcome at the same time the disadvantages of 2D cell culture and in vivo animal models and fill in the gap between them.Given the liposarcoma rarity,we believe that 3D cell culture techniques,including 3D cell cultures/co-cultures,and Patient-Derived tumor Organoids(PDOs),represent a promising approach to facilitate liposarcoma investigation and elucidate its molecular mechanisms and effective therapy development.In this review,we first provide a general overview of 3D cell cultures compared to 2D cell cultures.We then focus on one of the recent 3D cell culture applications,Patient-Derived Organoids(PDOs),summarizing and discussing several PDO methodologies.Finally,we discuss the current and future applications of PDOs to sarcoma,particularly in the field of liposarcoma.展开更多
An earlier version of this paper has been presented online at the annual Conference of Canadian Sociological Association,during the Congress of Humanities and Social Sciences in Toronto in June 2025.It explores how ra...An earlier version of this paper has been presented online at the annual Conference of Canadian Sociological Association,during the Congress of Humanities and Social Sciences in Toronto in June 2025.It explores how rap music in Quebec,particularly through the work of the rap artist Webster and his collective Limoilou Starz(LS),serves as a tool for meaning-making,political expression,and resistance against racial profiling and police brutality experienced by Black youth in Quebec City.The study focuses on the song“SPVQ”(Service de police de la Ville de Québec)as a case study to analyze the encoding and reception of anti-racist messages within rap culture.Webster draws from a global tradition of socio-political rap-like that of African-American rappers KRS-One,LL Cool J,and Tupac-while anchoring his critique in the local realities of Limoilou,a marginalized,racially diverse district of Quebec City.Through a narrative,figurative,semantic,and ideological analysis of the song,the paper reveals how Webster articulates themes of police brutality,structural discrimination,economic marginalization,and resistance.The rapper’s message is both a form of testimony and a civic intervention.His broader activism is analyzed through ethnographic techniques-including participant observation and interviews during workshops,media appearances,and online campaigns.It translates these messages into tangible social practices.To explain the persistence of these injustices,the paper situates the issue within Quebec’s interculturalism framework,which ostensibly promotes dialogue and integration but often masks or even reinforces structural racism.This model maintains a symbolic majority/minority duality and conditions inclusion on conformity to a dominant White Francophone identity,thus rendering racialized youth as perpetual outsiders.展开更多
Chengdu teahouses,as core public spaces in marketplace society,have undergone transformative reconstruction-from“containers of everyday life”to“containers of commercial traffic and digital flows”-during the proces...Chengdu teahouses,as core public spaces in marketplace society,have undergone transformative reconstruction-from“containers of everyday life”to“containers of commercial traffic and digital flows”-during the process of modernization.Employing spatial archaeology as a methodology,combined with fieldwork and analysis of historical documents,this study systematically examines the diachronic evolution of architectural forms,functional orientations,and social networks within Chengdu teahouses.The study reveals the logic of spatial reconstruction under the interplay of multiple forces,including cultural heritage preservation,capital-driven development,and technological intervention.The findings identify three paradigms of spatial transformation in teahouses.First,heritage specimenization,which reinforces the continuity of collective memory through symbolic extraction but risks diminishing the vitality of everyday social interactions.Second,consumption upgrading,which caters to the demands of emerging groups through iterative business models yet necessitates vigilance against spatial differentiation eroding marketplace inclusivity.Third,digital parasitism,which expands communicative dimensions through technological empowerment but confronts the risk of flattening localized knowledge.These paradigms reflect both adaptive responses of traditional spaces to contemporary pressure and the tension of reconstruction imposed by instrumental rationality on marketplace networks.The study demonstrates that spatial transformation in Chengdu teahouses is not unidirectional alienation but rather a multifaceted configuration where the continuity of tradition coexists with innovative practices amid functional diversification.This research advocates for striking a balance between the preservation of traditional spaces and modern renewal and explores organic integration approaches for traditional and modern elements,thereby providing a theoretical framework and practical insights for the transformation of traditional public spaces.展开更多
Rice callus suspension culture(RCSC)has been shown to have anticancer activity based on cytotoxic activity on human colon and lung cancer cell lines.In the present study,the effect of RCSC on the expression of protein...Rice callus suspension culture(RCSC)has been shown to have anticancer activity based on cytotoxic activity on human colon and lung cancer cell lines.In the present study,the effect of RCSC on the expression of proteins in lung(A549)and colon(HT29)cancer cell lines was examined by using proteomics analysis.The protein-protein interaction study of differentially expressed proteins was done by using the Search Tool for the Retrieval of Interacting Genes(STRING),and the results showed that the proteins interacting with each other belong to different pathways.展开更多
The Yangtze River Economic Belt is the birthplace of the world s rice farming culture,and is the origin of common rice(Oryza sativa L.),wild rice(Oryza Rufipogon Griff.),medicinal rice(Oryza officinalis Wall.ex G.Watt...The Yangtze River Economic Belt is the birthplace of the world s rice farming culture,and is the origin of common rice(Oryza sativa L.),wild rice(Oryza Rufipogon Griff.),medicinal rice(Oryza officinalis Wall.ex G.Watt),and wart-grain rice(Oryza granate Nees et Arn.ex Hook.f.).Rice culture is the main theme of agricultural culture in this economic belt and an outstanding representative of excellent traditional Chinese culture.This study provides an overview of the history of rice cultivation in the belt from the prehistory,post-history and modern progress.It studies the agricultural cultural heritage resources of the whole rice industry chain from the biological genetic resources,world cultural heritage,key cultural relics protection units,national archaeological site parks,movable cultural relics,important agricultural cultural heritage,intangible cultural heritage,China time-honored brands,historical names,traditional markers,tribute culture,archival and documentary heritage.It also analyzes the six main problems in the inheritance and development of rice farming culture,and proposes 8 strategies,including promoting the inheritance and innovative development of excellent traditional Chinese culture through the inheritance of rice farming culture,promoting the Exploration of the Origins of Chinese Civilization Project through rice farming culture,and creating the Yangtze River National Cultural Park with rice farming culture as the main theme.展开更多
This paper conducts a systematic analysis of the pathways for integrating excellent traditional culture into ideological and political education in higher education institutions,exploring four dimensions:incorporating...This paper conducts a systematic analysis of the pathways for integrating excellent traditional culture into ideological and political education in higher education institutions,exploring four dimensions:incorporating classical content into the curriculum system,enhancing teachers’cultural literacy,implementing practice-oriented teaching that integrates knowledge and action,and fostering a campus cultural environment conducive to such integration.The study proposes a“three-tier screening-multi-dimensional integration-dynamic update”mechanism for textbook integration,a“theoretical foundation-practical refinement-reflective enhancement”model for teacher development,a“goal-oriented-activity design-effect evaluation”system for practical teaching,and a“spatial renovation-activity planning-digital empowerment”strategy for cultural environment creation.By establishing a systematic operational process and a closed-loop training mechanism,the integration of traditional culture and ideological and political education is achieved,effectively enhancing the cultural depth and educational effectiveness of ideological and political teaching,and laying a solid foundation for universities to fulfill their fundamental mission of cultivating virtue and fostering talent.展开更多
With the in-depth advancement of rural revitalization and urban-rural integration strategies,the integration of agriculture,culture,and tourism has become an important path for promoting high-quality development in me...With the in-depth advancement of rural revitalization and urban-rural integration strategies,the integration of agriculture,culture,and tourism has become an important path for promoting high-quality development in metropolitan suburbs.Taking Cihui Subdistrict in Wuhan as an example,this research systematically sorts out its resource endowments,development models,and implementation effectiveness of the agriculture-culture-tourism integration through field research and case analysis.It further delves into the existing problems,such as insufficient planning and coordination,weak factor support,and insufficient industrial integration,along with their underlying causes.On such basis,targeted countermeasures are proposed from the aspects of scientific planning,industrial collaboration,talent introduction and cultivation,brand building,and policy optimization.The study aims to build an integrated development system of agriculture,culture,and tourism tailored to the characteristics of metropolitan suburbs,providing theoretical references and policy inspiration for similar regions.展开更多
In the context of rural revitalization,the landscape design of public spaces in Enshi Xiaomaopoying Village presents both new opportunities and challenges.This study explores the significance of integrating regional c...In the context of rural revitalization,the landscape design of public spaces in Enshi Xiaomaopoying Village presents both new opportunities and challenges.This study explores the significance of integrating regional culture into rural public spaces.Field research has been conducted to assess the current utilization of regional culture in the public spaces of Xiaomaopoying Village.Subsequently,design optimization strategies are analyzed and proposed.Additionally,the practical application of regional cultural elements is examined,in order to offer guidance for the application of cultural creation of traditional characteristic villages in rural public spaces.展开更多
基金supported by the Science and Technology Innovation Program of Fujian Agriculture and Forestry University,China(Grant No.KFB22045)the General Program of Natural Science Foundation Fujian,China(Grant No.2023J01460).
文摘The doubled haploid(DH)technique accelerates homozygosity by inducing chromosome doubling in haploid embryos derived from hybrid plants.This approach offers significant advantages over conventional rice breeding methods by shortening the breeding cycle and enabling rapid development of pure homozygous lines.Anther culture(AC)has been established as an efficient and successful method for producing DH plants via androgenesis in rice.However,despite its success in japonica rice.
文摘The fertile soil of the countryside is also rich in artistic inspiration.“If farmers pick up a brush,they can become artists.If one devotes oneself wholeheartedly to something,a stroke of genius will follow,”said Miao Huixin,a cultural envoy to Shengfeng Village in Youchegang Town,Xiuzhou District,Jiaxing City,Zhejiang Province.
文摘This paper looks back to the development of plant tissue culture in China in the last century. Since 1934, tissue culture studies in China has kept up with the international development in the fields. Progress has been made by Chinese in nearly every branches of tissue culture, including in vitro organogenesis, shoot tip culture, anther culture, ovary culture, endosperm culture, protoplast culture as well as mass cell culture. On the basis of reviewing the articles written by Chinese on plant tissue culture, the internationally recognized contributions are specially mentioned. The applications of plant tissue culture to agriculture and industry in China are also introduced.
文摘At dawn in Wufu Village,in Dinghu District of Zhaoqing City,Guangdong Province,the newly restored Chaoyangli Cultural Retreat is already welcoming its first visitors.A visitor surnamed Chen,who has travelled from Guangzhou with his child,stops in front of the Chaxi Academy to admire local intangible cultural heritage crafts.
文摘This work examines media through a decolonial lens,centring Dagba$epistemologies to demonstrate how African languages,cultures,and traditions can transform how we think of knowledge.Focusing on film,television and radio in Ghanaian African languages,the book argues for a decolonial politics and praxis in co-creating knowledge with Indigenous communities.It critically links the struggles of global majority countries,showing how colonialism and imperialism obstruct liberatory futures,while foregrounding African language media as vital tools for cultural and epistemological decolonisation.Heralded as part of an unstoppable shift away from Eurocentrism,the book is distinguished by its radical feminist subaltern analysis and its deep grounding in lived experience from Northern Ghana.By excavating local agency within the digital mediascape,Mohammed o!ers a crucial historical and political-economic theorisation of media practice and consumption.
文摘In the year 2026,the world cultural exchanges will continue to develop,while the international public opinion environment remainsdive rseand complex.Looking back at this pivotal moment in history,the life journey of Sunny Wu,Chairman of the Hong Kong Doyens’Club,resonates perfectly with the national development.
基金funded by Beijing Natural Science Foundation(6252001)Guangdong Basic and Applied Basic Research Foundation(2022A1515140021)Natural Science Foundation of China(31871772).
文摘Probiotics can regulate gut microbes to maintain human health.However,the sensitivity of probiotics to environmental conditions reduces their bioavailability.In contrast,the formation of probiotic biofilm provides a natural physical barrier against external interference.Our previous study established a dynamic culture system of the biofilm-state Bifidobacterium adolescentis Gr19(B-DC-B.adolescentis Gr19),forming higher density and more structurally stable biofilms,which enhanced its potential probiotic properties in vivo.Thus,the protective effect and mechanism of B-DC-B.adolescentis Gr19 on lipopolysaccharide(LPS)-induced intestinal barrier dysfunction were investigated in this study.The results showed that B-DC-B.adolescentis Gr19 not only had high resistance and adhesion activity,but also improved the intestinal barrier by increasing goblet cells and promoting the expression of tight junction(TJ)-related proteins.Moreover,B-DC-B.adolescentis Gr19 effectively attenuated intestinal barrier injury in Caco-2 cells by improving intestinal permeability and integrity.Remarkably,B-DC-B.adolescentis Gr19 enhanced expression of TJ proteins,restored localization of cytoskeleton and reduced intestinal inflammation by suppressing the Ras homolog family member A/Rho-associated coiled-coil-forming kinases/nuclear factor kappa B/myosin light chain kinase/myosin light chain(RhoA/ROCK/NF-κB/MLCK/MLC)pathway.Therefore,B-DC-B.adolescentis Gr19 plays a key role in mitigating LPS-induced intestinal barrier dysfunction.Overall,the present study provides a theoretical basis for ameliorating intestinal barrier dysfunction and developing novel functional foods by using biofilm-state probiotics under dynamic culture.
基金supported by the Natural Science Foundation of Guangdong Province(No.2021B1515120053)Guangdong Basic and Applied Basic Research Foundation(Grant No.2024A1515140166).
文摘Background:Therapeutic responses of breast cancer vary among patients and lead to drug resistance and recurrence due to the heterogeneity.Current preclinical models,however,are inadequate for predicting individual patient responses towards different drugs.This study aimed to investigate the patient-derived breast cancer culture models for drug sensitivity evaluations.Methods:Tumor and adjacent tissues from female breast cancer patients were collected during surgery.Patient-derived breast cancer cells were cultured using the conditional reprogramming technique to establish 2D models.The obtained patient-derived conditional reprogramming breast cancer(CRBC)cells were subsequently embedded in alginate-gelatin methacryloyl hydrogel microspheres to form 3D culture models.Comparisons between 2D and 3D models were made using immunohistochemistry(tumor markers),MTS assays(cell viability),flow cytometry(apoptosis),transwell assays(migration),and Western blotting(protein expression).Drug sensitivity tests were conducted to evaluate patient-specific responses to anti-cancer agents.Results:2D and 3D culture models were successfully established using samples from eight patients.The 3D models retained histological and marker characteristics of the original tumors.Compared to 2D cultures,3D models exhibited increased apoptosis,enhanced drug resistance,elevated stem cell marker expression,and greater migration ability—features more reflective of in vivo tumor behavior.Conclusion:Patient-derived 3D CRBC models effectively mimic the in vivo tumor microenvironment and demonstrate stronger resistance to anti-cancer drugs than 2D models.These hydrogel-based models offer a cost-effective and clinically relevant platform for drug screening and personalized breast cancer treatment.
基金“A Study on the Value and Path of Integrating Excellent Traditional Chinese Culture Into Intercultural Communication Courses”(ZD2024)a project by the Beijing Higher Education Association,as well as“A Study on the Path of Empowering the Integration of Excellent Traditional Chinese Culture Into Intercultural Communication Courses With Generative AI”(2024),an institutional project of Beijing International Studies University.
文摘This paper undertakes a systematic combing of the development of research on integrating Chinese culture into foreign language education in China from the 1980s to 2025,dividing it into three stages:cultural attachment,cultural compensation,and cultural symbiosis,and reveals the logical shift of the research from the dominance of target language culture to the construction of the subjectivity of Chinese culture.Through quantitative and qualitative analysis of 435 CSSCI papers,three core themes are extracted:what to integrate,why to integrate,and how to integrate.This paper critically analyzes three pairs of contradictions:the imbalance between instrumentality and humanism,the separation of national narrative and individual expression,and the disconnection between traditional inheritance and modern transformation.It is proposed that future research should reconstruct the educational logic based on the Chinese context,integrate the national and individual dimensions,and build a dialogue mechanism between tradition and modernity,so as to provide theoretical and practical reference for the construction of a foreign language education system with Chinese characteristics.
基金Supported by the Bio&Medical Technology Development Program of the National Research Foundation Funded by the Korean Government(MSIT),No.RS-2023-00223501Hallym University Research Fund.
文摘BACKGROUND The unique survival environment of Helicobacter pylori(H.pylori)presents challenges in establishing appropriate in vitro culture conditions.The fastidious nature of H.pylori and its relatively low growth rate emphasize the need for customized culture conditions,which are essential for both research and clinical applications.AIM To identify the optimal liquid culture medium for H.pylori using both reference and clinical strains.METHODS Nine H.pylori strains were incubated in 10 different broth media commonly used for bacterial cultures under microaerophilic conditions.Bacterial concentrations were estimated using serial dilutions and the pour plate method.A range of H.pylori initial inoculum concentrations was tested for each strain.For growth evaluation,optical density at 600 nm was measured at 24,48,and 72 hours.The growth trends of H.pylori were compared among strains and media.RESULTS All H.pylori strains grew successfully over time,regardless of the initial inoculum concentration.The specific growth trends and the broth that yielded the highest optical density value at each time point varied with strain.The reference strains(H.pylori 26695,ATCC 43504,and J99)consistently exhibited robust growth,whereas the clinical strains displayed distinct media preferences.Overall,bacterial growth was higher in chopped meat carbohydrate broth,Columbia broth,and fastidious anaerobe broth than in Brucella broth after 72 hours.CONCLUSION Chopped meat carbohydrate broth was the most effective for H.pylori liquid culture,and Columbia broth and fastidious anaerobe broth also supported greater growth than Brucella broth.
文摘Yunhe County in Lishui City,southwestern Zhejiang Province,doesn’t have any Disney castles.Instead,it has built bridges to the world with wood.Known as“China’s town of wooden toys,”accounting for about 40 percent of the global wooden toy market,Yunhe’s story began with a small factory established in 1970.Today,it has grown into a distinctive industrial cluster with an annual output value exceeding 8 billion yuan($1.15 billion),exporting products to 82 countries and regions.
基金funded under the National key research and development plan(2021YFC2101404)Chinese Academy of Engineering Strategic Research and Consulting Project(2023-XZ-79,2022-30-19).
文摘Cell cultured meat has been extensively studied as an environmentally friendly,energy-saving and more effective technology.However,there are many technical bottlenecks,especially the regulatory mechanism and manufacturing method of in vitro myogenesis.Based on an edible modified silk protein scaffold,with 3D culturing,in situ differentiated and transcriptome analysis,this study describes novel scaffolds and fabrication methods for cell cultured meat.The results showed that the effective space and utilization efficiency for cell culture of the scaffold is 26–1000 that of the traditional culture dish;it could form a tissue-like structure.Transcriptomics revealed the regulatory pathways and key factors of different cycles.It clarifies that the multi-cycle process of myoblast myogenesis in vitro is different from the single feedback regulation in vivo.More importantly,a novel scaffold-based cell cultured meat manufacturing method was developed,further develop a new tissue culture solution that is different from existing cell culture meat production.For manufacturing processes,it provides a new cell culture meat technology system,provides a theoretical basis for the regulation of cell proliferation and muscle growth,and lays the technical foundation for in situ tissue culture of cell cultured meat in vitro.
文摘Liposarcoma is one of the most common soft tissue sarcomas,however,its occurrence rate is still rare compared to other cancers.Due to its rarity,in vitro experiments are an essential approach to elucidate liposarcoma pathobiology.Conventional cell culture-based research(2D cell culture)is still playing a pivotal role,while several shortcomings have been recently under discussion.In vivo,mouse models are usually adopted for pre-clinical analyses with expectations to overcome the issues of 2D cell culture.However,they do not fully recapitulate human dedifferentiated liposarcoma(DDLPS)characteristics.Therefore,three-dimensional(3D)culture systems have been the recent research focus in the cell biology field with the expectation to overcome at the same time the disadvantages of 2D cell culture and in vivo animal models and fill in the gap between them.Given the liposarcoma rarity,we believe that 3D cell culture techniques,including 3D cell cultures/co-cultures,and Patient-Derived tumor Organoids(PDOs),represent a promising approach to facilitate liposarcoma investigation and elucidate its molecular mechanisms and effective therapy development.In this review,we first provide a general overview of 3D cell cultures compared to 2D cell cultures.We then focus on one of the recent 3D cell culture applications,Patient-Derived Organoids(PDOs),summarizing and discussing several PDO methodologies.Finally,we discuss the current and future applications of PDOs to sarcoma,particularly in the field of liposarcoma.
文摘An earlier version of this paper has been presented online at the annual Conference of Canadian Sociological Association,during the Congress of Humanities and Social Sciences in Toronto in June 2025.It explores how rap music in Quebec,particularly through the work of the rap artist Webster and his collective Limoilou Starz(LS),serves as a tool for meaning-making,political expression,and resistance against racial profiling and police brutality experienced by Black youth in Quebec City.The study focuses on the song“SPVQ”(Service de police de la Ville de Québec)as a case study to analyze the encoding and reception of anti-racist messages within rap culture.Webster draws from a global tradition of socio-political rap-like that of African-American rappers KRS-One,LL Cool J,and Tupac-while anchoring his critique in the local realities of Limoilou,a marginalized,racially diverse district of Quebec City.Through a narrative,figurative,semantic,and ideological analysis of the song,the paper reveals how Webster articulates themes of police brutality,structural discrimination,economic marginalization,and resistance.The rapper’s message is both a form of testimony and a civic intervention.His broader activism is analyzed through ethnographic techniques-including participant observation and interviews during workshops,media appearances,and online campaigns.It translates these messages into tangible social practices.To explain the persistence of these injustices,the paper situates the issue within Quebec’s interculturalism framework,which ostensibly promotes dialogue and integration but often masks or even reinforces structural racism.This model maintains a symbolic majority/minority duality and conditions inclusion on conformity to a dominant White Francophone identity,thus rendering racialized youth as perpetual outsiders.
基金supported by the Research Center for Chengdu History and Chengdu Literature[CLWX24004]the Centre for Southeast Asia Economic and Culture Studies[DNY2415]the Sichuan Landscape and Recreation Research Center[JGYQ2025027].
文摘Chengdu teahouses,as core public spaces in marketplace society,have undergone transformative reconstruction-from“containers of everyday life”to“containers of commercial traffic and digital flows”-during the process of modernization.Employing spatial archaeology as a methodology,combined with fieldwork and analysis of historical documents,this study systematically examines the diachronic evolution of architectural forms,functional orientations,and social networks within Chengdu teahouses.The study reveals the logic of spatial reconstruction under the interplay of multiple forces,including cultural heritage preservation,capital-driven development,and technological intervention.The findings identify three paradigms of spatial transformation in teahouses.First,heritage specimenization,which reinforces the continuity of collective memory through symbolic extraction but risks diminishing the vitality of everyday social interactions.Second,consumption upgrading,which caters to the demands of emerging groups through iterative business models yet necessitates vigilance against spatial differentiation eroding marketplace inclusivity.Third,digital parasitism,which expands communicative dimensions through technological empowerment but confronts the risk of flattening localized knowledge.These paradigms reflect both adaptive responses of traditional spaces to contemporary pressure and the tension of reconstruction imposed by instrumental rationality on marketplace networks.The study demonstrates that spatial transformation in Chengdu teahouses is not unidirectional alienation but rather a multifaceted configuration where the continuity of tradition coexists with innovative practices amid functional diversification.This research advocates for striking a balance between the preservation of traditional spaces and modern renewal and explores organic integration approaches for traditional and modern elements,thereby providing a theoretical framework and practical insights for the transformation of traditional public spaces.
文摘Rice callus suspension culture(RCSC)has been shown to have anticancer activity based on cytotoxic activity on human colon and lung cancer cell lines.In the present study,the effect of RCSC on the expression of proteins in lung(A549)and colon(HT29)cancer cell lines was examined by using proteomics analysis.The protein-protein interaction study of differentially expressed proteins was done by using the Search Tool for the Retrieval of Interacting Genes(STRING),and the results showed that the proteins interacting with each other belong to different pathways.
基金Supported by Social Science Foundation of Hubei Province(HBSKJJ20243227)Doctoral Initiation Project of Hubei University of Science and Technology(BK201819).
文摘The Yangtze River Economic Belt is the birthplace of the world s rice farming culture,and is the origin of common rice(Oryza sativa L.),wild rice(Oryza Rufipogon Griff.),medicinal rice(Oryza officinalis Wall.ex G.Watt),and wart-grain rice(Oryza granate Nees et Arn.ex Hook.f.).Rice culture is the main theme of agricultural culture in this economic belt and an outstanding representative of excellent traditional Chinese culture.This study provides an overview of the history of rice cultivation in the belt from the prehistory,post-history and modern progress.It studies the agricultural cultural heritage resources of the whole rice industry chain from the biological genetic resources,world cultural heritage,key cultural relics protection units,national archaeological site parks,movable cultural relics,important agricultural cultural heritage,intangible cultural heritage,China time-honored brands,historical names,traditional markers,tribute culture,archival and documentary heritage.It also analyzes the six main problems in the inheritance and development of rice farming culture,and proposes 8 strategies,including promoting the inheritance and innovative development of excellent traditional Chinese culture through the inheritance of rice farming culture,promoting the Exploration of the Origins of Chinese Civilization Project through rice farming culture,and creating the Yangtze River National Cultural Park with rice farming culture as the main theme.
文摘This paper conducts a systematic analysis of the pathways for integrating excellent traditional culture into ideological and political education in higher education institutions,exploring four dimensions:incorporating classical content into the curriculum system,enhancing teachers’cultural literacy,implementing practice-oriented teaching that integrates knowledge and action,and fostering a campus cultural environment conducive to such integration.The study proposes a“three-tier screening-multi-dimensional integration-dynamic update”mechanism for textbook integration,a“theoretical foundation-practical refinement-reflective enhancement”model for teacher development,a“goal-oriented-activity design-effect evaluation”system for practical teaching,and a“spatial renovation-activity planning-digital empowerment”strategy for cultural environment creation.By establishing a systematic operational process and a closed-loop training mechanism,the integration of traditional culture and ideological and political education is achieved,effectively enhancing the cultural depth and educational effectiveness of ideological and political teaching,and laying a solid foundation for universities to fulfill their fundamental mission of cultivating virtue and fostering talent.
基金Sponsored by Innovation and Entrepreneurship Training Program for College Students of Wuhan Polytechnic University(202510496165)Youth Project of the Education Department of Hubei Province(24Q195).
文摘With the in-depth advancement of rural revitalization and urban-rural integration strategies,the integration of agriculture,culture,and tourism has become an important path for promoting high-quality development in metropolitan suburbs.Taking Cihui Subdistrict in Wuhan as an example,this research systematically sorts out its resource endowments,development models,and implementation effectiveness of the agriculture-culture-tourism integration through field research and case analysis.It further delves into the existing problems,such as insufficient planning and coordination,weak factor support,and insufficient industrial integration,along with their underlying causes.On such basis,targeted countermeasures are proposed from the aspects of scientific planning,industrial collaboration,talent introduction and cultivation,brand building,and policy optimization.The study aims to build an integrated development system of agriculture,culture,and tourism tailored to the characteristics of metropolitan suburbs,providing theoretical references and policy inspiration for similar regions.
文摘In the context of rural revitalization,the landscape design of public spaces in Enshi Xiaomaopoying Village presents both new opportunities and challenges.This study explores the significance of integrating regional culture into rural public spaces.Field research has been conducted to assess the current utilization of regional culture in the public spaces of Xiaomaopoying Village.Subsequently,design optimization strategies are analyzed and proposed.Additionally,the practical application of regional cultural elements is examined,in order to offer guidance for the application of cultural creation of traditional characteristic villages in rural public spaces.
