以青鳉为模式生物,通过表型观测和miRNAs测序,探究三氯生对青鳉胚胎发育的毒性机制.结果表明:5μg/L三氯生暴露15d后,青鳉胚胎(仔鱼)出现孵化率降低、体长变短、卵黄囊肿大、心包囊肿、生殖嵴缩短、眼直径减小、眼轴延长和眼间距异常.mi...以青鳉为模式生物,通过表型观测和miRNAs测序,探究三氯生对青鳉胚胎发育的毒性机制.结果表明:5μg/L三氯生暴露15d后,青鳉胚胎(仔鱼)出现孵化率降低、体长变短、卵黄囊肿大、心包囊肿、生殖嵴缩短、眼直径减小、眼轴延长和眼间距异常.miRNAs测序共鉴定出6个差异表达miRNAs,其中2个显著上调,4个显著下调,这些miRNAs共靶向3166个基因.GO(Gene Ontology)功能分析表明,显著富集的条目涉及神经肌肉接头突触的特异性形成、肌节组装、肌肉牵张感知等过程.KEGG(Kyoto Encyclopedia of Genes and Genomes)通路分析发现19条显著富集通路,包括轴突导向、PI3K-Akt信号通路和PPAR信号通路等,这些通路与代谢调控、神经系统功能、运动能力、致癌风险和发育毒性密切相关.展开更多
Halogens are common in industrial thermal processes and can induce formation of toxic organic pollutants.Currently,the specific effects of coexisting halogens on toxic pollutant formation are unclear.In this study,we ...Halogens are common in industrial thermal processes and can induce formation of toxic organic pollutants.Currently,the specific effects of coexisting halogens on toxic pollutant formation are unclear.In this study,we found the boosting effects of halogens on organic pollutants formation during thermal processes.In situ electron paramagnetic resonance spectroscopy was used to distinguish the organic free radical intermediates of organic pollutants during thermal processes.The pure chlorine-containing reaction system dominantly induce the formation of highly chlorinated phenoxy radicals,whereas the pure bromine-containing reaction system induce the formation of semiquinone radicals.The results provide clear evidence for the molecular mechanisms by which halogen coexistence boosts pollutants formation.Coexisting halogens made the reactions much more complex.Bromine atom radicals(Br·)can enhance the level of active chlorine atoms radicals(Cl·),then(1)facilitating highly chlorinated organic radicals and toxic pollutants formation,(2)activating polycyclic aromatic hydrocarbons molecules for subsequent ring growth and(3)accelerating oxidation reactions.Combining the identification of organic free radical intermediates and characteristics of products,detailed mechanisms of halogens on the formations of organic pollutants during thermal-related processes were clarified,which can be helpful for their efficient control in the widespread bromines and chlorines co-existence reaction system.展开更多
文摘以青鳉为模式生物,通过表型观测和miRNAs测序,探究三氯生对青鳉胚胎发育的毒性机制.结果表明:5μg/L三氯生暴露15d后,青鳉胚胎(仔鱼)出现孵化率降低、体长变短、卵黄囊肿大、心包囊肿、生殖嵴缩短、眼直径减小、眼轴延长和眼间距异常.miRNAs测序共鉴定出6个差异表达miRNAs,其中2个显著上调,4个显著下调,这些miRNAs共靶向3166个基因.GO(Gene Ontology)功能分析表明,显著富集的条目涉及神经肌肉接头突触的特异性形成、肌节组装、肌肉牵张感知等过程.KEGG(Kyoto Encyclopedia of Genes and Genomes)通路分析发现19条显著富集通路,包括轴突导向、PI3K-Akt信号通路和PPAR信号通路等,这些通路与代谢调控、神经系统功能、运动能力、致癌风险和发育毒性密切相关.
基金supported by the National Key Research and Development Program of China(No.2023YFC3707004)the National Natural Science Foundation of China(Nos.22376204,22076201 and 92143201)+1 种基金the Strategic Priority Research Program of the Chinese Academy of Sciences(Nos.XDB0750400,XDB0750100 and XDB0750000)the Second Tibetan Plateau Scientific Expedition and Research Program(STEP)(No.2019 QZKK0605).
文摘Halogens are common in industrial thermal processes and can induce formation of toxic organic pollutants.Currently,the specific effects of coexisting halogens on toxic pollutant formation are unclear.In this study,we found the boosting effects of halogens on organic pollutants formation during thermal processes.In situ electron paramagnetic resonance spectroscopy was used to distinguish the organic free radical intermediates of organic pollutants during thermal processes.The pure chlorine-containing reaction system dominantly induce the formation of highly chlorinated phenoxy radicals,whereas the pure bromine-containing reaction system induce the formation of semiquinone radicals.The results provide clear evidence for the molecular mechanisms by which halogen coexistence boosts pollutants formation.Coexisting halogens made the reactions much more complex.Bromine atom radicals(Br·)can enhance the level of active chlorine atoms radicals(Cl·),then(1)facilitating highly chlorinated organic radicals and toxic pollutants formation,(2)activating polycyclic aromatic hydrocarbons molecules for subsequent ring growth and(3)accelerating oxidation reactions.Combining the identification of organic free radical intermediates and characteristics of products,detailed mechanisms of halogens on the formations of organic pollutants during thermal-related processes were clarified,which can be helpful for their efficient control in the widespread bromines and chlorines co-existence reaction system.
