鱼油因较低的水溶性限制了其应用。该研究通过在鱼油中添加不同浓度的磷脂,构建鱼油-磷脂自乳化体系,表征其理化性质,并结合药代动力学分析,系统评价该体系的体内吸收效果。结果表明,鱼油中添加6%(质量分数)磷脂能形成良好的自乳化乳液...鱼油因较低的水溶性限制了其应用。该研究通过在鱼油中添加不同浓度的磷脂,构建鱼油-磷脂自乳化体系,表征其理化性质,并结合药代动力学分析,系统评价该体系的体内吸收效果。结果表明,鱼油中添加6%(质量分数)磷脂能形成良好的自乳化乳液,可显著降低油水界面张力和乳液油滴粒径,抑制液滴聚并,增强乳液的稳定性;药代动力学实验表明,该磷脂-鱼油自乳化体系能显著缩短鱼油中二十碳五烯酸(eicosapentaenoic acid,EPA)和二十二碳六烯酸(docosahexaenoic acid,DHA)的达峰时间(T_(max)最快至1.5 h)并提高最大血药浓度(C_(max)达1.39 mg/mL),显著改善不同类型鱼油的体内吸收效果,其中EPA的0~24 h的血药浓度-时间曲线下面积(area under the plasma concentration-time curve from time 0 to 24 hours,AUC_(0-24h))可提升至2.66~3.74倍,DHA的AUC_(0-24h)可提升至3.20~7.44倍。该研究为高生物利用度的鱼油制剂产品开发提供了数据支撑和技术参考。展开更多
Krill oil is effective in reducing blood lipid levels,particularly in individuals with severe hyperlipidemia.However,poor water insolubility and stability limited its usage.This study investigated a method for encapsu...Krill oil is effective in reducing blood lipid levels,particularly in individuals with severe hyperlipidemia.However,poor water insolubility and stability limited its usage.This study investigated a method for encapsulating Antarctic krill oil using alginate(ALG)and gelatin(GLN)to enhance its stability and bioactivity.The encapsulation efficiency,functional group integrity,swelling rate,and lipid-lowering activity were assessed.Results indicated that the optimal encapsulation conditions were identified with an ALG:GLN ratio of 2:1(m/m),coagulation bath of 9%CaCl_(2),and a nozzle size of 750μm,resulting in 69.34%encapsulation efficiency.Fourier-transform infrared spectroscopy confirmed successful encapsulation.The ALG-GLN shell materials enriched astaxanthin in krill oil and protected it from harsh gastric conditions,enabling targeted intestinal release.In a high-fat diet-induced rat model,krill oil microcapsules significantly reduced triglycerides(TG),total cholesterol(TC),and low-density lipoprotein-cholesterol(LDL-C)levels while increasing high-density lipoprotein-cholesterol(HDL-C)levels compared to unencapsulated krill oil.Additionally,the microcapsules elevated nitric oxide(NO)levels,enhanced superoxide dismutase(SOD)activity,and reduced malondialdehyde(MDA)levels,liver and perirenal fat weight.Therefore,encapsulating Antarctic krill oil in alginate-gelatin hydrogel offers a promising strategy for managing hyperlipidemia and associated metabolic disorders.展开更多
文摘鱼油因较低的水溶性限制了其应用。该研究通过在鱼油中添加不同浓度的磷脂,构建鱼油-磷脂自乳化体系,表征其理化性质,并结合药代动力学分析,系统评价该体系的体内吸收效果。结果表明,鱼油中添加6%(质量分数)磷脂能形成良好的自乳化乳液,可显著降低油水界面张力和乳液油滴粒径,抑制液滴聚并,增强乳液的稳定性;药代动力学实验表明,该磷脂-鱼油自乳化体系能显著缩短鱼油中二十碳五烯酸(eicosapentaenoic acid,EPA)和二十二碳六烯酸(docosahexaenoic acid,DHA)的达峰时间(T_(max)最快至1.5 h)并提高最大血药浓度(C_(max)达1.39 mg/mL),显著改善不同类型鱼油的体内吸收效果,其中EPA的0~24 h的血药浓度-时间曲线下面积(area under the plasma concentration-time curve from time 0 to 24 hours,AUC_(0-24h))可提升至2.66~3.74倍,DHA的AUC_(0-24h)可提升至3.20~7.44倍。该研究为高生物利用度的鱼油制剂产品开发提供了数据支撑和技术参考。
基金funded by the National Key Research and Development Program of China(2023YFF1103804)the Academic Research Projects of Beijing Union University(ZK20202516).
文摘Krill oil is effective in reducing blood lipid levels,particularly in individuals with severe hyperlipidemia.However,poor water insolubility and stability limited its usage.This study investigated a method for encapsulating Antarctic krill oil using alginate(ALG)and gelatin(GLN)to enhance its stability and bioactivity.The encapsulation efficiency,functional group integrity,swelling rate,and lipid-lowering activity were assessed.Results indicated that the optimal encapsulation conditions were identified with an ALG:GLN ratio of 2:1(m/m),coagulation bath of 9%CaCl_(2),and a nozzle size of 750μm,resulting in 69.34%encapsulation efficiency.Fourier-transform infrared spectroscopy confirmed successful encapsulation.The ALG-GLN shell materials enriched astaxanthin in krill oil and protected it from harsh gastric conditions,enabling targeted intestinal release.In a high-fat diet-induced rat model,krill oil microcapsules significantly reduced triglycerides(TG),total cholesterol(TC),and low-density lipoprotein-cholesterol(LDL-C)levels while increasing high-density lipoprotein-cholesterol(HDL-C)levels compared to unencapsulated krill oil.Additionally,the microcapsules elevated nitric oxide(NO)levels,enhanced superoxide dismutase(SOD)activity,and reduced malondialdehyde(MDA)levels,liver and perirenal fat weight.Therefore,encapsulating Antarctic krill oil in alginate-gelatin hydrogel offers a promising strategy for managing hyperlipidemia and associated metabolic disorders.
