Nine novel fluorescent dyes of Benzoxanthene-3,4-dicarboxylic anhydride were synthesized through bromination,condensation,reduction,cyclization and imidation with 1,8-naphthalic anhydride as raw material.Their fluores...Nine novel fluorescent dyes of Benzoxanthene-3,4-dicarboxylic anhydride were synthesized through bromination,condensation,reduction,cyclization and imidation with 1,8-naphthalic anhydride as raw material.Their fluorescent and dyeing properties were studied.They can be used to color polyester fiber in fluorescent greenish-yellow hues of great brilliance with fastness to light:6～7．展开更多
Organic small molecule fluorophores have been widely used in biology and biochemistry to study cellular structures and processes at high spatial and temporal resolution.Small-molecule dyes offer various benefits,such ...Organic small molecule fluorophores have been widely used in biology and biochemistry to study cellular structures and processes at high spatial and temporal resolution.Small-molecule dyes offer various benefits,such as high photostability,low molecular weight,and great biocompatibility.However,the poor brightness of most of conventional dyes in biological environments limits their use in high-quality superresolution fluorescence imaging.Chemists have conceived and developed many methods to enhance the brightness of fluorophores,including structural alterations that raise extinction coefficients and quantum yields.This review outlines current attempts and substantial advances achieved by chemists to improve the brightness of organic small-molecule fluorescent dyes,such as scaffold rigidification and twisted intramolecular charge transfer(TICT)inhibition.We think that this review will help researchers understand the chemical mechanisms involved in increasing the brightness of fluorophores for biological applications.展开更多
文摘Nine novel fluorescent dyes of Benzoxanthene-3,4-dicarboxylic anhydride were synthesized through bromination,condensation,reduction,cyclization and imidation with 1,8-naphthalic anhydride as raw material.Their fluorescent and dyeing properties were studied.They can be used to color polyester fiber in fluorescent greenish-yellow hues of great brilliance with fastness to light:6～7．
基金supported by the National Science Foundation for Distinguished Young Scholars(No.22325401)the National Natural Science Foundation of China(No.22404049)the China Postdoctoral Science Foundation(No.2024M750866)。
文摘Organic small molecule fluorophores have been widely used in biology and biochemistry to study cellular structures and processes at high spatial and temporal resolution.Small-molecule dyes offer various benefits,such as high photostability,low molecular weight,and great biocompatibility.However,the poor brightness of most of conventional dyes in biological environments limits their use in high-quality superresolution fluorescence imaging.Chemists have conceived and developed many methods to enhance the brightness of fluorophores,including structural alterations that raise extinction coefficients and quantum yields.This review outlines current attempts and substantial advances achieved by chemists to improve the brightness of organic small-molecule fluorescent dyes,such as scaffold rigidification and twisted intramolecular charge transfer(TICT)inhibition.We think that this review will help researchers understand the chemical mechanisms involved in increasing the brightness of fluorophores for biological applications.
