The effects of extraction time,alkaline concentration,and the addition of cartilage on the extraction of mucopolysaccharides from shark fin cartilage digested with NaOH were investigated.The main components of mucopol...The effects of extraction time,alkaline concentration,and the addition of cartilage on the extraction of mucopolysaccharides from shark fin cartilage digested with NaOH were investigated.The main components of mucopolysaccharides during the degrading process were analyzed by cellulose polyacetate strip electrophoresis and agarose gel electrophoresis.The results showed that the extraction of mucopolysaccharides was increased with the increase of extraction time,alkaline concentration and the addition of cartilage.According to the different electrophoretic migrations,the preparative mucopolysaccharides composed of different polysaccharides with electronegativity can be divided into two parts,the chondroitin sulfate with higher migration and the other with lower migration.The lower part is unstable and easily degraded with alkaline solution.展开更多
文摘The effects of extraction time,alkaline concentration,and the addition of cartilage on the extraction of mucopolysaccharides from shark fin cartilage digested with NaOH were investigated.The main components of mucopolysaccharides during the degrading process were analyzed by cellulose polyacetate strip electrophoresis and agarose gel electrophoresis.The results showed that the extraction of mucopolysaccharides was increased with the increase of extraction time,alkaline concentration and the addition of cartilage.According to the different electrophoretic migrations,the preparative mucopolysaccharides composed of different polysaccharides with electronegativity can be divided into two parts,the chondroitin sulfate with higher migration and the other with lower migration.The lower part is unstable and easily degraded with alkaline solution.
文摘应用Box-Behnken设计-响应面法优化猴头菇粗多糖的提取工艺。以提取时间、液料比、温度为影响粗多糖提取率的主要考察因素,通过Box-Behnken试验设计,以猴头菇粗多糖提取率为响应值,应用Designexpert 7.0软件对结果进行二次多项式模型拟合,绘制等高线图和响应面图,比较各因素交互作用对提取率影响的强弱,得出最优工艺,并对其进行验证。结果显示最佳工艺条件为:提取时间3.8 h,液料比25 m L/g,提取温度81℃。验证实验显示猴头菇粗多糖提取率为3.09%,实测值与预测值接近。研究结果说明响应曲面法建立的模型预测性良好,能合理地优化猴头菇中粗多糖的提取工艺。
