本研究旨在探讨原花青素-铁网络涂层包裹乳酸菌后对发酵黑莓汁的影响。通过原花青素(Pc)与铁离子反应生成金属-多酚网络,将其包裹植物乳植杆菌BC17形成BC17@Pc-Fe保护结构,并应用于发酵黑莓汁中,研究原花青素-铁包裹乳酸菌后对黑莓汁发...本研究旨在探讨原花青素-铁网络涂层包裹乳酸菌后对发酵黑莓汁的影响。通过原花青素(Pc)与铁离子反应生成金属-多酚网络,将其包裹植物乳植杆菌BC17形成BC17@Pc-Fe保护结构,并应用于发酵黑莓汁中,研究原花青素-铁包裹乳酸菌后对黑莓汁发酵过程中的活菌数、总糖、还原糖、总酚、花色苷、pH、总酸以及抗氧化能力的影响。结果表明,包裹后的BC17的粒径由1990±156 nm增加至5560±901 nm,所带电荷由-13.77±1.44 mV增加至-4.05±1.48 mV;扫描电子显微镜研究显示,由原花青素和铁离子构成的金属-多酚网络成功包裹BC17。在发酵黑莓汁过程中,BC17@Pc-Fe组的活菌数能达到7.59±0.03 lg CFU/mL,显著(P<0.05)高于BC17组的活菌数(6.64±0.03 lg CFU/mL),其总糖和还原糖含量、总酚和花色苷含量与BC17组相比分别升高了13.29%±2.81%和6.49%±1.01%、4.47%±2.02%和33.96%±4.26%。同时,与BC17组相比,BC17@Pc-Fe组pH降低了2.72%±0.01%、总酸含量升高了4.60%±0.03%。经过抗氧化能力评估,BC17@Pc-Fe组的DPPH自由基清除率、ABTS+自由基清除率、·OH清除率以及亚铁离子还原能力比BC17组分别提高19.33%±2.94%、29.17%±2.82%、12.31%±7.22%和11.31%±0.03%,原花青素-铁网络涂层包裹BC17可以有效提高黑莓发酵汁的抗氧化能力。本研究通过改善乳酸菌生长和发酵性能来提升黑莓发酵汁的品质,为乳酸菌发酵果汁类产品提供了新思路。展开更多
The first example of Nd@C_(3)N_(4)-photoredox/chlorine dual catalyzed alkylation with unactivated alkanes as the alkyl sources has been developed,which allows for the synthesis of various 4-alkylated cyclic sulfonyl k...The first example of Nd@C_(3)N_(4)-photoredox/chlorine dual catalyzed alkylation with unactivated alkanes as the alkyl sources has been developed,which allows for the synthesis of various 4-alkylated cyclic sulfonyl ketimines.In this process,chlorine functions as both a redox and hydrogen atom transfer catalyst.The synergism of the reversible Nd^(2+)/Nd^(3+)and Cl^(ˉ)/Cl˙redox pairs significantly enhances overall photocatalytic efficiency.The in vitro anticancer activity of 4-alkylated products was evaluated by using the CCK8assay against both human choroidal melanoma(MUM-2B)and lung cancer(A549)cell.Compound 3da showed approximately triple the potency of 5-fluorouracil.展开更多
文摘本研究旨在探讨原花青素-铁网络涂层包裹乳酸菌后对发酵黑莓汁的影响。通过原花青素(Pc)与铁离子反应生成金属-多酚网络,将其包裹植物乳植杆菌BC17形成BC17@Pc-Fe保护结构,并应用于发酵黑莓汁中,研究原花青素-铁包裹乳酸菌后对黑莓汁发酵过程中的活菌数、总糖、还原糖、总酚、花色苷、pH、总酸以及抗氧化能力的影响。结果表明,包裹后的BC17的粒径由1990±156 nm增加至5560±901 nm,所带电荷由-13.77±1.44 mV增加至-4.05±1.48 mV;扫描电子显微镜研究显示,由原花青素和铁离子构成的金属-多酚网络成功包裹BC17。在发酵黑莓汁过程中,BC17@Pc-Fe组的活菌数能达到7.59±0.03 lg CFU/mL,显著(P<0.05)高于BC17组的活菌数(6.64±0.03 lg CFU/mL),其总糖和还原糖含量、总酚和花色苷含量与BC17组相比分别升高了13.29%±2.81%和6.49%±1.01%、4.47%±2.02%和33.96%±4.26%。同时,与BC17组相比,BC17@Pc-Fe组pH降低了2.72%±0.01%、总酸含量升高了4.60%±0.03%。经过抗氧化能力评估,BC17@Pc-Fe组的DPPH自由基清除率、ABTS+自由基清除率、·OH清除率以及亚铁离子还原能力比BC17组分别提高19.33%±2.94%、29.17%±2.82%、12.31%±7.22%和11.31%±0.03%,原花青素-铁网络涂层包裹BC17可以有效提高黑莓发酵汁的抗氧化能力。本研究通过改善乳酸菌生长和发酵性能来提升黑莓发酵汁的品质,为乳酸菌发酵果汁类产品提供了新思路。
基金supported by grants from the Provincial Natural Science Foundation of Hunan(No.2023JJ60335)。
文摘The first example of Nd@C_(3)N_(4)-photoredox/chlorine dual catalyzed alkylation with unactivated alkanes as the alkyl sources has been developed,which allows for the synthesis of various 4-alkylated cyclic sulfonyl ketimines.In this process,chlorine functions as both a redox and hydrogen atom transfer catalyst.The synergism of the reversible Nd^(2+)/Nd^(3+)and Cl^(ˉ)/Cl˙redox pairs significantly enhances overall photocatalytic efficiency.The in vitro anticancer activity of 4-alkylated products was evaluated by using the CCK8assay against both human choroidal melanoma(MUM-2B)and lung cancer(A549)cell.Compound 3da showed approximately triple the potency of 5-fluorouracil.
