生物传感器已经成为实时监测特定小分子和精确控制生物系统中基因表达的强大工具。用于1,4-丁二胺生物合成的高通量传感器可以极大地提高1,4-丁二胺高产菌株的筛选效率。为研究调整生物传感器特性的策略,本研究开发了一种以转录调节因子...生物传感器已经成为实时监测特定小分子和精确控制生物系统中基因表达的强大工具。用于1,4-丁二胺生物合成的高通量传感器可以极大地提高1,4-丁二胺高产菌株的筛选效率。为研究调整生物传感器特性的策略,本研究开发了一种以转录调节因子PuuR为基础的1,4-丁二胺生物传感器,其同源的操作子puuO被组装在大肠杆菌组成型启动子PgapA中,以控制下游的高能绿色荧光蛋白(superfolder green fluorescent protein,sfGFP)作为报告蛋白表达。最终该传感器在1,4-丁二胺浓度处于0–50 mmol/L时GFP/OD600值与1,4-丁二胺浓度之间能稳定地表现出线性关系。本研究采用大肠杆菌基因组中不同强度的启动子对1,4-丁二胺生物传感器进行分子改造,探究并改进基于PuuR的1,4-丁二胺生物传感器的功能性质,为高通量筛选高产1,4-丁二胺的工程菌株奠定了基础。展开更多
踝泵运动是一种临床患者进行血栓预防和康复训练的重要手段,指导患者进行正确的踝泵运动不仅可以大大降低下肢静脉血栓栓塞的发生,而且可以加快患者康复进程。目前,实际康复治疗中,由于缺乏对病人踝泵运动情况的有效监测,在对康复训练...踝泵运动是一种临床患者进行血栓预防和康复训练的重要手段,指导患者进行正确的踝泵运动不仅可以大大降低下肢静脉血栓栓塞的发生,而且可以加快患者康复进程。目前,实际康复治疗中,由于缺乏对病人踝泵运动情况的有效监测,在对康复训练过程和效果无法掌握情况下,往往无法制定出最佳康复训练计划。因此,本文设计了一种基于力和角度的踝泵运动实时监测系统,来解决以上问题。该运动监测系统采用ESP32实时采集压力传感器和姿态传感器的数据,同时利用无线方式发送到上位机,在上位机通过对数据的相应处理,得出踝泵训练过程中足底用力和踝关节角度变化情况。试验结果表明,该监测系统通过采集力与角度的实时变化,可以实现踝泵运动的有效监控。Ankle pump exercise is an important method for preventing thrombosis and promoting rehabilitation in clinical patients. Guiding patients to perform proper ankle pump exercises can significantly reduce the occurrence of lower limb venous thromboembolism and accelerate the recovery process. However, in current rehabilitation treatments, due to the lack of effective monitoring of patients’ ankle pump exercise activities, it is often difficult to develop the optimal rehabilitation plan when the training process and outcomes are not well understood. Therefore, this paper designs a real-time monitoring system for ankle pump exercise based on force and angle, aiming to address the above issues. The system utilizes an ESP32 microcontroller to collect real-time data from pressure sensors and posture sensors. The collected data is wirelessly transmitted to an upper computer, where it is processed to analyze changes in plantar force and ankle joint angles during ankle pump exercises. Experimental results show that this monitoring system effectively monitors ankle pump exercises by capturing the real-time changes in force and angle.展开更多
文摘生物传感器已经成为实时监测特定小分子和精确控制生物系统中基因表达的强大工具。用于1,4-丁二胺生物合成的高通量传感器可以极大地提高1,4-丁二胺高产菌株的筛选效率。为研究调整生物传感器特性的策略,本研究开发了一种以转录调节因子PuuR为基础的1,4-丁二胺生物传感器,其同源的操作子puuO被组装在大肠杆菌组成型启动子PgapA中,以控制下游的高能绿色荧光蛋白(superfolder green fluorescent protein,sfGFP)作为报告蛋白表达。最终该传感器在1,4-丁二胺浓度处于0–50 mmol/L时GFP/OD600值与1,4-丁二胺浓度之间能稳定地表现出线性关系。本研究采用大肠杆菌基因组中不同强度的启动子对1,4-丁二胺生物传感器进行分子改造,探究并改进基于PuuR的1,4-丁二胺生物传感器的功能性质,为高通量筛选高产1,4-丁二胺的工程菌株奠定了基础。
文摘踝泵运动是一种临床患者进行血栓预防和康复训练的重要手段,指导患者进行正确的踝泵运动不仅可以大大降低下肢静脉血栓栓塞的发生,而且可以加快患者康复进程。目前,实际康复治疗中,由于缺乏对病人踝泵运动情况的有效监测,在对康复训练过程和效果无法掌握情况下,往往无法制定出最佳康复训练计划。因此,本文设计了一种基于力和角度的踝泵运动实时监测系统,来解决以上问题。该运动监测系统采用ESP32实时采集压力传感器和姿态传感器的数据,同时利用无线方式发送到上位机,在上位机通过对数据的相应处理,得出踝泵训练过程中足底用力和踝关节角度变化情况。试验结果表明,该监测系统通过采集力与角度的实时变化,可以实现踝泵运动的有效监控。Ankle pump exercise is an important method for preventing thrombosis and promoting rehabilitation in clinical patients. Guiding patients to perform proper ankle pump exercises can significantly reduce the occurrence of lower limb venous thromboembolism and accelerate the recovery process. However, in current rehabilitation treatments, due to the lack of effective monitoring of patients’ ankle pump exercise activities, it is often difficult to develop the optimal rehabilitation plan when the training process and outcomes are not well understood. Therefore, this paper designs a real-time monitoring system for ankle pump exercise based on force and angle, aiming to address the above issues. The system utilizes an ESP32 microcontroller to collect real-time data from pressure sensors and posture sensors. The collected data is wirelessly transmitted to an upper computer, where it is processed to analyze changes in plantar force and ankle joint angles during ankle pump exercises. Experimental results show that this monitoring system effectively monitors ankle pump exercises by capturing the real-time changes in force and angle.
