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腰椎间盘突出症模型大鼠的疼痛与抑郁共病情况及氟西汀疗效的实验研究 被引量:3
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作者 甘国强 薛莲 《颈腰痛杂志》 2021年第3期365-368,共4页
目的探讨腰椎间盘突出症大鼠模型中疼痛和抑郁共病情况及氟西汀的治疗效果。方法将56只Wistar大鼠随机分为对照组、假手术组、模型组、氟西汀组四组,对照组大鼠未经任何特殊处理;假手术组经手术造模暴露右侧L 5背根神经节;模型组和氟西... 目的探讨腰椎间盘突出症大鼠模型中疼痛和抑郁共病情况及氟西汀的治疗效果。方法将56只Wistar大鼠随机分为对照组、假手术组、模型组、氟西汀组四组,对照组大鼠未经任何特殊处理;假手术组经手术造模暴露右侧L 5背根神经节;模型组和氟西汀组经手术造模形成椎间盘突出、压迫神经根。术后4周开始,氟西汀组大鼠给予20 mg/kg氟西汀灌胃,2次/d;对照组、假手术组、模型组给予等量生理盐水灌胃。术前、术后2周、4周、6周时,评估大鼠的机械缩爪阈值(MWT)和抑郁症行为(糖水偏爱实验、强迫游泳实验和旷场实验);末次给药完毕、检测完行为学实验后,采用酶联免疫吸附法(ELISA法检)测海马和前额叶皮质肿瘤坏死因子α(TNF-ɑ)和5羟色胺(5-HT)水平。结果术后2周、4周、6周时,模型组和氟西汀组大鼠MWT阈值均显著低于对照组和假手术组(P<0.05);术后2周和4周时,模型组和氟西汀组MWT阈值差异无统计学意义(P>0.05);术后6周时氟西汀组MWT阈值显著高于模型组(P<0.05)。术后2周、4周、6周时,模型组和氟西汀组糖水偏爱率、穿越格子数低于对照组和假手术组(P<0.05),漂浮不动时间高于对照组和模型组(P<0.05);术后2周和4周时,模型组和氟西汀组大鼠的糖水偏爱率、漂浮不动时间、穿越格子数差异均无统计学意义(P>0.05);术后6周时,氟西汀组糖水偏爱率和穿越格子数高于模型组,漂浮不动时间低于模型组,差异均有统计学意义(P<0.05)。模型组和氟西汀组前额叶和海马组织TNF-ɑ水平均显著高于对照组和假手术组(P<0.05),5-HT水平均低于对照组和假手术组;氟西汀组前额叶和海马组织TNF-ɑ水平均显著低于模型组(P<0.05),5-HT水平均显著高于模型组(P<0.05)。结论腰椎间盘突出症大鼠模型存在抑郁和疼痛共病的表现,氟西汀可通过改善脑组织5-HT和TNF-ɑ水平改善抑郁和疼痛表现,为腰椎间盘突出症疼痛和抑郁共病的治疗提供了理论基础。 展开更多
关键词 腰椎间盘突出症 模型 疼痛 抑郁 氟西汀
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Experimental Study on Decolorization and Degradation of Reactive Brilliant Red X-3B in a White Rot Fungal Biofilm Reactor 被引量:5
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作者 HUANG Min sheng, HUANG Rong, CHENG Yong qian, ZHANG Guo ying School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200072, China 《Journal of Shanghai University(English Edition)》 CAS 2001年第3期260-264,共5页
Experimental results of an azo dye(reactive brilliant red X 3B, RBR X 3B) decolorization and degradation in a white rot fungal biofilm reactor were introduced and discussed. The fungal biofilm reactor is highly pote... Experimental results of an azo dye(reactive brilliant red X 3B, RBR X 3B) decolorization and degradation in a white rot fungal biofilm reactor were introduced and discussed. The fungal biofilm reactor is highly potential for dye decolorization and degradation with the highest decoloring rate of 95% within 96 hours reaction time at initial pH 4.5 under high nitrogen level (HN) (24 mmol/L ammonium tartrate) condition. Experimental conditions, such as nutrient nitrogen levels in reaction mixture and initial pH, significantly affected dye decolorization and degradation. Effluents from this biofilm reactor can be well treated to meet the discharging requirements by use of chemical flocculation.RBR X 3B was first absorbed onto fungal biomass and then degraded gradually. The SH 13 fungus monopolized the biofilm throughout the experiments, though the reactor was exposed to open air for 4 months. 展开更多
关键词 white rot fungi dye decolorization DEGRADATION
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