Hard clams Mercenaria mercenaria were introduced from America to China in 2000. In this paper, the contents of 18 common acids,protein, fat as well as vitamines and mineral ions in edible part of hard calm were analyz...Hard clams Mercenaria mercenaria were introduced from America to China in 2000. In this paper, the contents of 18 common acids,protein, fat as well as vitamines and mineral ions in edible part of hard calm were analyzed. The results showed that the contents of 18 common acids and the essential amino acid were 52.42% and 40.88% respectively and the average score of essential amino acid was 96.4±11.3 according to the FAO/WHO suggested level (1973), and in which the smelled amino acid takes about 48.30%. The content of protein in edible part of hard clam was higher(60.69%) and the content of fat was much lower(5.60%) than that of some bivalves. The results also indicated that the edible part of hard clam was also rich in amino acids, vitamins(A, B1,B2,B5,B6), and mineral ions such as Ca,Zn,Fe,Mn, etc. Hard clam was suggested to be with higher nutritional value which was good for human health.展开更多
文摘【目的】基于转录组测序分析低氧胁迫下缢蛏鳃组织的重要调控通路,探究低氧胁迫对缢蛏能量代谢相关基因的影响,以明确缢蛏应对低氧胁迫的响应机制。【方法】对缢蛏进行低氧胁迫(溶解氧2.0±0.2 mg/L)12 h(L1)和24 h(L2),以常氧条件(溶解氧7.0±0.2 mg/L)为对照组(Control),采集缢蛏鳃组织进行转录组测序,筛选差异表达基因(DEGs)并进行KEGG信号通路富集分析。采用实时荧光定量PCR检测低氧胁迫0、4、8、12、24、48和96 h缢蛏鳃组织中能量代谢相关基因的相对表达量。【结果】L1 vs Control比较组有5445个DEGs,L2 vs Control比较组有2980个DEGs。KEGG信号通路富集分析结果表明,L1 vs Control比较组共有498个上调DEGs注释到177条通路,显著(P<0.05,下同)富集的前10条通路包括钙信号通路、c GMP-PKG信号通路、C型凝集素受体信号通路等;L2 vs Control比较组共有583个上调DEGs注释到224条通路,其富集的代谢通路较L1 vs Control比较组增多,包括类固醇生物合成、亚油酸代谢、视黄醇代谢、泛醌和其他萜类醌的生物合成等通路。从L2 vs Control比较组DEGs中筛选出缢蛏鳃组织中响应低氧胁迫的能量代谢相关基因,主要涉及糖酵解/糖异生、TCA循环、AMPK信号通路、HIF-1信号通路等重要的调控通路。实时荧光定量PCR检测结果表明,随着低氧胁迫时间的延长,缢蛏鳃组织中己糖激酶基因(HK)、磷酸烯醇丙酮酸羧化激酶基因(PEPCK)、磷酸甘油酸激酶基因(PGK)的相对表达量呈先升高后降低再升高的变化趋势,在低氧胁迫4 h较0 h明显升高,且在低氧胁迫96 h呈高表达;葡萄糖转运蛋白-1基因(GLUT1)、烯醇化酶基因(ENO)的相对表达量在低氧胁迫4 h较0 h显著降低,在低氧胁迫12 h降至最低值,低氧胁迫持续24 h后GLUT1和ENO基因的相对表达量逐渐上升,在低氧胁迫96 h达最高值且与0 h差异显著。【结论】低氧胁迫促进缢蛏糖酵解/糖异生、HIF-1信号通路等调控通路中的基因上调,对缢蛏能量代谢存在显著影响,推测低氧胁迫下缢蛏通过糖酵解途径维持能量供应,PEPCK、HK、GLUT1、PGK等基因参与能量代谢调节及低氧胁迫响应。
文摘Hard clams Mercenaria mercenaria were introduced from America to China in 2000. In this paper, the contents of 18 common acids,protein, fat as well as vitamines and mineral ions in edible part of hard calm were analyzed. The results showed that the contents of 18 common acids and the essential amino acid were 52.42% and 40.88% respectively and the average score of essential amino acid was 96.4±11.3 according to the FAO/WHO suggested level (1973), and in which the smelled amino acid takes about 48.30%. The content of protein in edible part of hard clam was higher(60.69%) and the content of fat was much lower(5.60%) than that of some bivalves. The results also indicated that the edible part of hard clam was also rich in amino acids, vitamins(A, B1,B2,B5,B6), and mineral ions such as Ca,Zn,Fe,Mn, etc. Hard clam was suggested to be with higher nutritional value which was good for human health.
