为探究池塘养殖凡纳对虾肠道微生物与环境微生物之间的关系,揭示养殖过程中微生物的变化规律,采用16S r DNA高通量测序和生物信息学方法,对水体、沉积物和对虾肠道微生物的组成、变化、功能及群落构建机制进行了分析研究。结果显示,凡...为探究池塘养殖凡纳对虾肠道微生物与环境微生物之间的关系,揭示养殖过程中微生物的变化规律,采用16S r DNA高通量测序和生物信息学方法,对水体、沉积物和对虾肠道微生物的组成、变化、功能及群落构建机制进行了分析研究。结果显示,凡纳对虾养殖池塘水体、沉积物和对虾肠道微生物存在差异且处于动态变化之中,水体中的优势微生物为变形菌门(Proteobacteria)和蓝细菌门(Cyanobacteria),沉积物中主要为拟杆菌门(Bacteroidota)和变形菌门,凡纳对虾肠道中主要为变形菌门和厚壁菌门(Firmicutes)。微生物来源(Source Tracker)分析表明,沉积物与肠道微生物的交换数量高于水体与肠道微生物的交换数量。群落构建机制分析结果显示,确定性过程主导水体微生物群落的构建,水体酸碱度和化学需氧量(COD)是最主要的环境因子;沉积物和肠道中的微生物群落由确定性过程与随机过程共同决定。结果表明,凡纳对虾肠道微生物的组成和功能受到环境微生物和环境因子等多种因素影响。展开更多
The conservation of aquatic systems is closely linked to the maintenance and improvement of aquaculture products'yield and quality.In this experiment,a high-quality aquatic system was established,comprising Litope...The conservation of aquatic systems is closely linked to the maintenance and improvement of aquaculture products'yield and quality.In this experiment,a high-quality aquatic system was established,comprising Litopenaeus vannamei as a model species and two strains of Bacillus(W1 and XYB4)combined with sodium humate under zero-water exchange conditions.The growth performance,enzyme activity,and aquatic system microbial environment of L.vannamei were analyzed.Results showed that the combination of sodium humate and Bacillus strains effectively enhanced environmental conditions for the growth and reproduction of heterotrophic bacteria while inhibiting the growth of Vibrio species,including green and yellow variants.Microbiome analysis showed that the group treated with Bacillus strains combined with sodium humate exhibited significantly higher relative abundances of Firmicutes and Actinobacteriota than the other groups.Correspondingly,this treatment group showed substantially enhanced weight gain rate,specific growth rate,survival rate,and feed coefficient.Moreover,the phenol oxidase,catalase,lysozyme,and superoxide dismutase indexes of shrimps subjected to Bacillus–sodium humate treatment were considerably higher than those of the control group.These findings confirm that the combination of Bacillus and sodium humate has beneficial effects on shrimp growth and aquatic system quality control,providing a promising strategy for enhancing the efficiency of shrimp farming and aquaculture.展开更多
文摘为探究池塘养殖凡纳对虾肠道微生物与环境微生物之间的关系,揭示养殖过程中微生物的变化规律,采用16S r DNA高通量测序和生物信息学方法,对水体、沉积物和对虾肠道微生物的组成、变化、功能及群落构建机制进行了分析研究。结果显示,凡纳对虾养殖池塘水体、沉积物和对虾肠道微生物存在差异且处于动态变化之中,水体中的优势微生物为变形菌门(Proteobacteria)和蓝细菌门(Cyanobacteria),沉积物中主要为拟杆菌门(Bacteroidota)和变形菌门,凡纳对虾肠道中主要为变形菌门和厚壁菌门(Firmicutes)。微生物来源(Source Tracker)分析表明,沉积物与肠道微生物的交换数量高于水体与肠道微生物的交换数量。群落构建机制分析结果显示,确定性过程主导水体微生物群落的构建,水体酸碱度和化学需氧量(COD)是最主要的环境因子;沉积物和肠道中的微生物群落由确定性过程与随机过程共同决定。结果表明,凡纳对虾肠道微生物的组成和功能受到环境微生物和环境因子等多种因素影响。
基金supported by the National Key R&D Program of China(No.2023YFD2401703)。
文摘The conservation of aquatic systems is closely linked to the maintenance and improvement of aquaculture products'yield and quality.In this experiment,a high-quality aquatic system was established,comprising Litopenaeus vannamei as a model species and two strains of Bacillus(W1 and XYB4)combined with sodium humate under zero-water exchange conditions.The growth performance,enzyme activity,and aquatic system microbial environment of L.vannamei were analyzed.Results showed that the combination of sodium humate and Bacillus strains effectively enhanced environmental conditions for the growth and reproduction of heterotrophic bacteria while inhibiting the growth of Vibrio species,including green and yellow variants.Microbiome analysis showed that the group treated with Bacillus strains combined with sodium humate exhibited significantly higher relative abundances of Firmicutes and Actinobacteriota than the other groups.Correspondingly,this treatment group showed substantially enhanced weight gain rate,specific growth rate,survival rate,and feed coefficient.Moreover,the phenol oxidase,catalase,lysozyme,and superoxide dismutase indexes of shrimps subjected to Bacillus–sodium humate treatment were considerably higher than those of the control group.These findings confirm that the combination of Bacillus and sodium humate has beneficial effects on shrimp growth and aquatic system quality control,providing a promising strategy for enhancing the efficiency of shrimp farming and aquaculture.
