[Objectives]The present study was conducted to investigate the change rule ofβ-fructofuranosidase gene expression and its enzyme activity in the midgut of 5 th instar silkworm(Bombyx mori),in order to provide a refer...[Objectives]The present study was conducted to investigate the change rule ofβ-fructofuranosidase gene expression and its enzyme activity in the midgut of 5 th instar silkworm(Bombyx mori),in order to provide a reference for illustrating the enzymatic mechanism of usingβ-fructofuranosidase to absorb sucrose nutrition from mulberry leaves.[Methods]Real-time fluorescent quantitative PCR was applied to analyze the expression of BmSuc1 and BmSuc2 in midgut of 5 th-instar silkworm larvae,meanwhile the activities ofβ-fructofuranosidase was determined.[Results]BmSuc1 was expressed in the midgut of 5 th-instar silkworm larvae at different developmental stages.Its expression was upregulated at the beginning of the 5 th instar and during the peak feeding period,whereas BmSuc2 expression remained very low throughout the entire 5 th instar.The activity ofβ-fructofuranosidase was relatively high during the peak feeding period of 5 th-instar larvae,showing a trend of increasing first and then decreasing.[Conclusions]The expression pattern of the BmSuc1 gene and the changes inβ-fructofuranosidase activity were generally consistent with the physiological process of sugar nutrient absorption and utilization from mulberry leaves in 5 th-instar silkworms.It suggests that BmSuc1,as a sucrose hydrolase gene,plays a major role in the digestion and absorption of sucrose nutrients from mulberry leaves in the midgut tissue.展开更多
桑枝是桑科植物桑(Mrous alba L.)的干燥嫩枝,具有祛风湿、利关节等功效,现代研究发现生物碱是桑枝中的主要活性物质。本文以高脂饮食小鼠为模型研究桑枝生物碱(SZ-A)对降脂的功效。实验结果表明:饲喂高脂饲料16周后,小鼠体重显著上升;...桑枝是桑科植物桑(Mrous alba L.)的干燥嫩枝,具有祛风湿、利关节等功效,现代研究发现生物碱是桑枝中的主要活性物质。本文以高脂饮食小鼠为模型研究桑枝生物碱(SZ-A)对降脂的功效。实验结果表明:饲喂高脂饲料16周后,小鼠体重显著上升;总生物碱干预后,SZ-A组小鼠体重显著低于模型组;理化指标分析发现,总生物碱显著降低了HFD小鼠血脂(TG、TC、LDL-C)、谷丙转氨酶、谷草转氨酶活性,有效缓解高脂饮食导致的小鼠肝脏病变;RT-PCR结果显示,总生物碱具有抑制CD36/PPARγ介导的脂质摄取,激活AMPK/ACC通路促进脂肪酸氧化,抑制SREBP1核转位减少脂质合成等降脂活性。本实验结果表明,桑枝生物碱SZ-A具有良好的降脂功效,能激活CD36/PPARγ与AMPK/ACC通路,抑制下游脂质代谢相关基因表达,发挥降脂功效和肝脏保护作用。展开更多
基金Supported by General Project of Yunnan Provincial Agricultural Basic Research Joint Special Project(202301BD070001-229)Yunnan Provincial Key R&D Program(202403AK140075)+1 种基金Modern Sericulture Industry Technology System of Yunan Province(KJTX-07)Honghe Comprehensive Test Station of National Sericulture Industry Technology System(CARS-18).
文摘[Objectives]The present study was conducted to investigate the change rule ofβ-fructofuranosidase gene expression and its enzyme activity in the midgut of 5 th instar silkworm(Bombyx mori),in order to provide a reference for illustrating the enzymatic mechanism of usingβ-fructofuranosidase to absorb sucrose nutrition from mulberry leaves.[Methods]Real-time fluorescent quantitative PCR was applied to analyze the expression of BmSuc1 and BmSuc2 in midgut of 5 th-instar silkworm larvae,meanwhile the activities ofβ-fructofuranosidase was determined.[Results]BmSuc1 was expressed in the midgut of 5 th-instar silkworm larvae at different developmental stages.Its expression was upregulated at the beginning of the 5 th instar and during the peak feeding period,whereas BmSuc2 expression remained very low throughout the entire 5 th instar.The activity ofβ-fructofuranosidase was relatively high during the peak feeding period of 5 th-instar larvae,showing a trend of increasing first and then decreasing.[Conclusions]The expression pattern of the BmSuc1 gene and the changes inβ-fructofuranosidase activity were generally consistent with the physiological process of sugar nutrient absorption and utilization from mulberry leaves in 5 th-instar silkworms.It suggests that BmSuc1,as a sucrose hydrolase gene,plays a major role in the digestion and absorption of sucrose nutrients from mulberry leaves in the midgut tissue.
文摘桑枝是桑科植物桑(Mrous alba L.)的干燥嫩枝,具有祛风湿、利关节等功效,现代研究发现生物碱是桑枝中的主要活性物质。本文以高脂饮食小鼠为模型研究桑枝生物碱(SZ-A)对降脂的功效。实验结果表明:饲喂高脂饲料16周后,小鼠体重显著上升;总生物碱干预后,SZ-A组小鼠体重显著低于模型组;理化指标分析发现,总生物碱显著降低了HFD小鼠血脂(TG、TC、LDL-C)、谷丙转氨酶、谷草转氨酶活性,有效缓解高脂饮食导致的小鼠肝脏病变;RT-PCR结果显示,总生物碱具有抑制CD36/PPARγ介导的脂质摄取,激活AMPK/ACC通路促进脂肪酸氧化,抑制SREBP1核转位减少脂质合成等降脂活性。本实验结果表明,桑枝生物碱SZ-A具有良好的降脂功效,能激活CD36/PPARγ与AMPK/ACC通路,抑制下游脂质代谢相关基因表达,发挥降脂功效和肝脏保护作用。
