背景:富血小板血浆因其含有多种细胞因子与其他蛋白成分且易制备、较安全,被作为治疗肌腱病的主要或辅助疗法。目前,对于富血小板血浆肌腱病的最佳治疗方案和疗效存在争议。目的:综述影响富血小板血浆对肌腱病疗效的相关因素以及富血小...背景:富血小板血浆因其含有多种细胞因子与其他蛋白成分且易制备、较安全,被作为治疗肌腱病的主要或辅助疗法。目前,对于富血小板血浆肌腱病的最佳治疗方案和疗效存在争议。目的:综述影响富血小板血浆对肌腱病疗效的相关因素以及富血小板血浆治疗的肌腱病机制,给出富血小板血浆联合辅助治疗肌腱病的方案。方法:检索PubMed、Web of Science、中国知网数据库,英文检索词为“PRP,tendinopathy,preparation,mechanism,combination therapy”,中文检索词为“富血小板血浆,肌腱病,制备,机制,联合疗法”,检索各数据库建库至2021年7月发表的相关文献。共检索到相关文献490篇,按照纳入与排除标准,最终纳入135篇文献进行总结。结果与结论:目前,富血小板血浆制备过程无统一标准,制备方法和成分、激活方式以及给药方式和施用次数等治疗方法差异可能影响富血小板血浆的疗效,最佳制备工艺参数尚未确定。评价富血小板血浆对肌腱病疗效的最佳方式是确定特定生长因子在肌腱修复相关分子信号通路中所起的作用,体外和体内基础研究发现,富血小板血浆具有促进组织再生和调节炎症的功能,可增加肌腱的力学强度、弹性模量和机械载荷。此外,肌腱病治疗的影响因素是多重的,应用富血小板血浆联合治疗能在一定意义上克服单一富血小板血浆存在的局限性,对于肌腱病更具现实意义。与组织工程学疗法、临床疗法联用可以在一定程度上提升富血小板血浆对肌腱病的治疗效果,取得更好的预后,进一步拓展富血小板血浆的应用范围。展开更多
Background： It has been reported that strenuous exercise increases the number of bone marrow-derived progenitor cells such as CD34＋ cells in the blood, but no previous studies have investigated the changes in circul...Background： It has been reported that strenuous exercise increases the number of bone marrow-derived progenitor cells such as CD34＋ cells in the blood, but no previous studies have investigated the changes in circulating CD34＋ cells following resistance exercise. This study tested the hypothesis that the number of CD34＋ cells in the blood would increase after eccentric exercise of the elbow flexors, but decrease in recovery, and the magnitude of the changes would be dependent on the magnitude of muscle damage. Methods： Nine men （28.0 ± 6.6 years） performed exercises consisting of 10 sets of six maximal voluntary eccentric contractions of the elbow flexors with their non-dominant arm. Six of them performed the same exercise with the same ann 4 weeks later. Changes in indirect markers of muscle damage were measured before, within 10 min after, and at 24, 48, 72, and 96 h after eccentric exercise. Differential leukocyte counts （total leukocytes, neutrophils, lymphocytes, monocytes） and CD34＋ cells in the blood were measured before, immediately after, and at 2, 24, 48, 72, and 96 h following the exercises. Results： After eccentric exercise, significant （p 〈 0.05） decreases in maximal voluntary isometric contraction torque and increases in delayed onset muscle soreness and plasma creatine kinase activity were observed. However, no significant changes in leukocytes and CD34＋ cells were evident. The changes in muscle damage markers were significantly （p 〈 0.05） smaller following the second exercise session as compared with the first exercise session, but the changes in leukocytes and CD34＋ cells were not significantly different between sessions. Conclusion： These results did not support the hypothesis, and showed that eccentric exercise-induced muscle damage to the elbow flexors did not influence the number of circulating CD34＋ cells.展开更多
Micro- injury （exercise-induced muscle damage, EIMD） will cause a temporary decline in muscle function, if not handled properly, it could cause more serious injury or overtraining, and even lead to the end of the at...Micro- injury （exercise-induced muscle damage, EIMD） will cause a temporary decline in muscle function, if not handled properly, it could cause more serious injury or overtraining, and even lead to the end of the athletes ＇ sports life. However, some studies have shown that an appropriate period of eccentric exercise training resulted in EIMD while promoted heart muscle fiber hypertrophy and corresponding muscle growth faster than exercise training, and there are the typical ＂ repetitive training effect＂ , ie after a period of the EIMD recovery, the muscle＇ s injury or delayed onset muscle soreness （DOMS） caused by the same movement is significantly reduced. So its deep study could help to guide the athlete to avoid or mitigate the negative effects which brought by EIMD, while could help to further clarify the process of the body to adapt to exercise training or mechanism.展开更多
文摘背景:富血小板血浆因其含有多种细胞因子与其他蛋白成分且易制备、较安全,被作为治疗肌腱病的主要或辅助疗法。目前,对于富血小板血浆肌腱病的最佳治疗方案和疗效存在争议。目的:综述影响富血小板血浆对肌腱病疗效的相关因素以及富血小板血浆治疗的肌腱病机制,给出富血小板血浆联合辅助治疗肌腱病的方案。方法:检索PubMed、Web of Science、中国知网数据库,英文检索词为“PRP,tendinopathy,preparation,mechanism,combination therapy”,中文检索词为“富血小板血浆,肌腱病,制备,机制,联合疗法”,检索各数据库建库至2021年7月发表的相关文献。共检索到相关文献490篇,按照纳入与排除标准,最终纳入135篇文献进行总结。结果与结论:目前,富血小板血浆制备过程无统一标准,制备方法和成分、激活方式以及给药方式和施用次数等治疗方法差异可能影响富血小板血浆的疗效,最佳制备工艺参数尚未确定。评价富血小板血浆对肌腱病疗效的最佳方式是确定特定生长因子在肌腱修复相关分子信号通路中所起的作用,体外和体内基础研究发现,富血小板血浆具有促进组织再生和调节炎症的功能,可增加肌腱的力学强度、弹性模量和机械载荷。此外,肌腱病治疗的影响因素是多重的,应用富血小板血浆联合治疗能在一定意义上克服单一富血小板血浆存在的局限性,对于肌腱病更具现实意义。与组织工程学疗法、临床疗法联用可以在一定程度上提升富血小板血浆对肌腱病的治疗效果,取得更好的预后,进一步拓展富血小板血浆的应用范围。
文摘Background： It has been reported that strenuous exercise increases the number of bone marrow-derived progenitor cells such as CD34＋ cells in the blood, but no previous studies have investigated the changes in circulating CD34＋ cells following resistance exercise. This study tested the hypothesis that the number of CD34＋ cells in the blood would increase after eccentric exercise of the elbow flexors, but decrease in recovery, and the magnitude of the changes would be dependent on the magnitude of muscle damage. Methods： Nine men （28.0 ± 6.6 years） performed exercises consisting of 10 sets of six maximal voluntary eccentric contractions of the elbow flexors with their non-dominant arm. Six of them performed the same exercise with the same ann 4 weeks later. Changes in indirect markers of muscle damage were measured before, within 10 min after, and at 24, 48, 72, and 96 h after eccentric exercise. Differential leukocyte counts （total leukocytes, neutrophils, lymphocytes, monocytes） and CD34＋ cells in the blood were measured before, immediately after, and at 2, 24, 48, 72, and 96 h following the exercises. Results： After eccentric exercise, significant （p 〈 0.05） decreases in maximal voluntary isometric contraction torque and increases in delayed onset muscle soreness and plasma creatine kinase activity were observed. However, no significant changes in leukocytes and CD34＋ cells were evident. The changes in muscle damage markers were significantly （p 〈 0.05） smaller following the second exercise session as compared with the first exercise session, but the changes in leukocytes and CD34＋ cells were not significantly different between sessions. Conclusion： These results did not support the hypothesis, and showed that eccentric exercise-induced muscle damage to the elbow flexors did not influence the number of circulating CD34＋ cells.
文摘Micro- injury （exercise-induced muscle damage, EIMD） will cause a temporary decline in muscle function, if not handled properly, it could cause more serious injury or overtraining, and even lead to the end of the athletes ＇ sports life. However, some studies have shown that an appropriate period of eccentric exercise training resulted in EIMD while promoted heart muscle fiber hypertrophy and corresponding muscle growth faster than exercise training, and there are the typical ＂ repetitive training effect＂ , ie after a period of the EIMD recovery, the muscle＇ s injury or delayed onset muscle soreness （DOMS） caused by the same movement is significantly reduced. So its deep study could help to guide the athlete to avoid or mitigate the negative effects which brought by EIMD, while could help to further clarify the process of the body to adapt to exercise training or mechanism.
