RT-PCR was employed to amplify the cDNA of HA gene of influenza A/Chicken/Guangdong/SS/94(H 9N 2)with a pair of degenerate primers and the cDNA were cloned into the T-T windows of plasmid pMD18-T.The inserts were sequ...RT-PCR was employed to amplify the cDNA of HA gene of influenza A/Chicken/Guangdong/SS/94(H 9N 2)with a pair of degenerate primers and the cDNA were cloned into the T-T windows of plasmid pMD18-T.The inserts were sequenced,at first time,and the results revealed that the HA gene had a long complete open reading frame and composed of 1,683 nucleotides,coding for 560 amino acids.The amino acid sequences of the HA connecting peptide revealed that A/Chicken/Guangdong/SS/94(H 9N 2)had X-X-X-R(X,not basic amino acid)at the proteolytic cleavage site.The molecular basis of the HA gene was compatible with not highly pathogenicity.Comparison of the degree of homology of HA gene showed 82%-98% nucleotide sequence and amino acid sequence homology among the isolate and the other H 9N 2 subtype AIV in GenBank. Thus, the HA gene of influenza A/Chicken/Guangdong/SS/94 belonged to H 9 subtype.展开更多
文摘RT-PCR was employed to amplify the cDNA of HA gene of influenza A/Chicken/Guangdong/SS/94(H 9N 2)with a pair of degenerate primers and the cDNA were cloned into the T-T windows of plasmid pMD18-T.The inserts were sequenced,at first time,and the results revealed that the HA gene had a long complete open reading frame and composed of 1,683 nucleotides,coding for 560 amino acids.The amino acid sequences of the HA connecting peptide revealed that A/Chicken/Guangdong/SS/94(H 9N 2)had X-X-X-R(X,not basic amino acid)at the proteolytic cleavage site.The molecular basis of the HA gene was compatible with not highly pathogenicity.Comparison of the degree of homology of HA gene showed 82%-98% nucleotide sequence and amino acid sequence homology among the isolate and the other H 9N 2 subtype AIV in GenBank. Thus, the HA gene of influenza A/Chicken/Guangdong/SS/94 belonged to H 9 subtype.
