PCV1 was isolated from IBRS-2 cells line,and its complete genomic sequence was cloned by PCR.Sequence analysis indicated that this PCV1 strain shares >98% nucleotide identity with the other PCV1 strains in GenBank....PCV1 was isolated from IBRS-2 cells line,and its complete genomic sequence was cloned by PCR.Sequence analysis indicated that this PCV1 strain shares >98% nucleotide identity with the other PCV1 strains in GenBank.Then double copy molecule clone(pSK2PCV1) was constructed and used to transfect PK-15 cell line.The results of indirect immunofluorescence(IIF) and RT-PCR suggested that pSK2PCV1 could form infectious virus after being transfected into PK-15 cells.展开更多
文摘PCV1 was isolated from IBRS-2 cells line,and its complete genomic sequence was cloned by PCR.Sequence analysis indicated that this PCV1 strain shares >98% nucleotide identity with the other PCV1 strains in GenBank.Then double copy molecule clone(pSK2PCV1) was constructed and used to transfect PK-15 cell line.The results of indirect immunofluorescence(IIF) and RT-PCR suggested that pSK2PCV1 could form infectious virus after being transfected into PK-15 cells.
