本试验旨在研究胚蛋注射不同剂量的赭曲霉毒素A(OTA)对出雏质量、空肠绒毛形态以及空肠炎性因子的含量影响。选用300枚受精种鸭蛋,分为5个处理组,对照组(注射0.1 mL 0.1 mol/L的NaHCO3溶液)和4个OTA剂量组(剂量分别为0.4、0.8、1.6、2.4...本试验旨在研究胚蛋注射不同剂量的赭曲霉毒素A(OTA)对出雏质量、空肠绒毛形态以及空肠炎性因子的含量影响。选用300枚受精种鸭蛋,分为5个处理组,对照组(注射0.1 mL 0.1 mol/L的NaHCO3溶液)和4个OTA剂量组(剂量分别为0.4、0.8、1.6、2.4 ng/g),每个处理6个重复,每个重复10枚蛋。在孵化的第13天进行胚蛋注射,出壳当天进行采样测定。结果表明,注射剂量为2.4 ng/g时降低了孵化率和出壳当天小肠相对长度(P<0.05);十二指肠、回肠、盲肠和直肠的相对长度随OTA注射剂量的升高呈线性降低(P<0.01);各肠段相对重量随着OTA剂量的升高均呈一次线性下降趋势(P<0.05);当注射剂量达到0.8ng/g时,空肠绒毛高度和绒隐比较对照组和0.4ng/g注射组显著降低,且空肠绒毛高度和绒隐比随着OTA剂量的增加呈线性下降趋势(P<0.05);随着OTA注射剂量的升高,雏鸭空肠炎性因子白介素-6(IL-6)含量呈线性上升趋势(P<0.01)。综上,胚蛋注射OTA会降低种蛋孵化率,减缓孵化过程中肝脏、胰腺和肠道的发育,并破坏雏鸭空肠组织形态,当注射剂量达到2.4 ng/g时会诱导空肠发生炎症。展开更多
Background AflatoxinB1(AFB_(1))is a prevalent contaminant in agricultural products,presenting significant risks to animal health.CotA laccase from Bacillus licheniformis has shown significant efficacy in degrading myc...Background AflatoxinB1(AFB_(1))is a prevalent contaminant in agricultural products,presenting significant risks to animal health.CotA laccase from Bacillus licheniformis has shown significant efficacy in degrading mycotoxins in vitro test.The efficacy of Bacillus CotA laccase in animals,however,remains to be confirmed.A 2×2 factorial design was used to investigate the effects of Bacillus CotA laccase level(0 or 1 U/kg),AFB_(1) challenge(challenged or unchal-lenged)and their interactions on ducks.The purpose of this study was to evaluate the efficacy of Bacillus CotA laccase in alleviatingAFB_(1) toxicosis of ducks.Results Bacillus CotA laccase alleviatedAFB_(1)-induced declines in growth performance of ducks accompanied by improved average daily gain(ADG)and lower feed/gain ratio(F/G).Bacillus CotA laccase amelioratedAFB_(1)-induced gut barrier dysfunctions and inflammation testified by increasing the jejunal villi height/crypt depth ratio(VH/CD)and the mRNA expression of tight junction protein 1(TJP1)and zonula occluden-1(ZO-1)as well as decreasing the expression of inflammation-related genes in the jejunum of ducks.Amino acid metabolome showed that Bacillus CotA laccase amelioratedAFB_(1)-induced amino acid metabolism disorders evidenced by increasing the level of glu-tamic acid in serum and upregulating the expression of amino acid transport related genes in jejunum of ducks.Bacil-lus CotA laccase amelioratedAFB_(1)-induced liver injury testified by suppressing oxidative stress,inhibiting apoptosis,and downregulating the expression of hepatic metabolic enzyme related genes of ducks.Moreover,Bacillus CotA laccase degradedAFB_(1) in digestive tract of ducks,resulting in the reduced absorption level ofAFB_(1) across intestinal epithelium testified by the decreased level ofAFB_(1)-DNA adduct in the liver,and the reduced content ofAFB_(1) residues in liver and feces of ducks.Conclusions Bacillus CotA laccase effectively improved the growth performance,intestinal health,amino acid metabolism and hepatic aflatoxin metabolism of ducks fedAFB_(1) diets,highlighting its potential as an efficient and safe feed enzyme forAFB_(1) degradation in animal production.展开更多
基金National Key Research and Development Program of China(2021YFC2103003)National Natural Science Foundation of China(31972604)+1 种基金Jinan Introductory Innovation Team Project(No.202228037)China Postdoctoral Science Foundation(2023M730998).
文摘Background AflatoxinB1(AFB_(1))is a prevalent contaminant in agricultural products,presenting significant risks to animal health.CotA laccase from Bacillus licheniformis has shown significant efficacy in degrading mycotoxins in vitro test.The efficacy of Bacillus CotA laccase in animals,however,remains to be confirmed.A 2×2 factorial design was used to investigate the effects of Bacillus CotA laccase level(0 or 1 U/kg),AFB_(1) challenge(challenged or unchal-lenged)and their interactions on ducks.The purpose of this study was to evaluate the efficacy of Bacillus CotA laccase in alleviatingAFB_(1) toxicosis of ducks.Results Bacillus CotA laccase alleviatedAFB_(1)-induced declines in growth performance of ducks accompanied by improved average daily gain(ADG)and lower feed/gain ratio(F/G).Bacillus CotA laccase amelioratedAFB_(1)-induced gut barrier dysfunctions and inflammation testified by increasing the jejunal villi height/crypt depth ratio(VH/CD)and the mRNA expression of tight junction protein 1(TJP1)and zonula occluden-1(ZO-1)as well as decreasing the expression of inflammation-related genes in the jejunum of ducks.Amino acid metabolome showed that Bacillus CotA laccase amelioratedAFB_(1)-induced amino acid metabolism disorders evidenced by increasing the level of glu-tamic acid in serum and upregulating the expression of amino acid transport related genes in jejunum of ducks.Bacil-lus CotA laccase amelioratedAFB_(1)-induced liver injury testified by suppressing oxidative stress,inhibiting apoptosis,and downregulating the expression of hepatic metabolic enzyme related genes of ducks.Moreover,Bacillus CotA laccase degradedAFB_(1) in digestive tract of ducks,resulting in the reduced absorption level ofAFB_(1) across intestinal epithelium testified by the decreased level ofAFB_(1)-DNA adduct in the liver,and the reduced content ofAFB_(1) residues in liver and feces of ducks.Conclusions Bacillus CotA laccase effectively improved the growth performance,intestinal health,amino acid metabolism and hepatic aflatoxin metabolism of ducks fedAFB_(1) diets,highlighting its potential as an efficient and safe feed enzyme forAFB_(1) degradation in animal production.
