Chakouyi(CKY)horses from the Qinghai-Xizang Plateau are well known for their unique lateral gaits and high-altitude adaptation,but genetic mechanisms underlying these phenotypes remain unclear.This study presents a co...Chakouyi(CKY)horses from the Qinghai-Xizang Plateau are well known for their unique lateral gaits and high-altitude adaptation,but genetic mechanisms underlying these phenotypes remain unclear.This study presents a comparison of 60 newly resequenced genomes of gaited CKY horses with 139 public genomes from 19 horse breeds.Population structure analyses(admixture,PCA,and neighbor-joining tree)reveal a close genetic relationship between CKY and other highland breeds(Tibetan and Chaidamu horses).Compared with other Chinese breeds,CKY horses present reduced nucleotide diversity(θπ)and lower inbreeding(FROHcoefficient),suggesting possible selective pressures.A key region on chromosome 23(Chr23:22.3-22.6 Mb)is associated with the lateral gaits and harbors a highly prevalent nonsense mutation(Chr 23:22,391,254 C>A,Ser301STOP)in the DMRT3 gene,with an 88%homozygosity rate,which is strongly correlated with the distinctive gait of CKY horses.Furthermore,selection signals reveal that the EPAS1 gene is related to high-altitude adaptation,and the CAT gene contributes to altitude resilience in CKY horses.These findings suggest that preserving genetic diversity is essential for maintaining the unique gaits and high-altitude adaptations of CKY horses.展开更多
成簇规则间隔短回文重复序列相关蛋白9 (The clustered regularly interspaced short palindromic repeats/CRISPR associated protein 9, CRISPR/Cas9)基因编辑技术是目前探索动物基因功能的有效手段之一。CRISPR/Cas9基因编辑技术表...成簇规则间隔短回文重复序列相关蛋白9 (The clustered regularly interspaced short palindromic repeats/CRISPR associated protein 9, CRISPR/Cas9)基因编辑技术是目前探索动物基因功能的有效手段之一。CRISPR/Cas9基因编辑技术表现出对宿主细胞内失活和/或消除整合前病毒DNA的显著潜力,对创造优良性状和提高生产性能具有深远的意义。尽管CRISPR/Cas9基因编辑技术在模式动物上已取得诸多成果,但应用于马的基因编辑研究仍处于起步阶段。该文就CRISPR/Cas9基因编辑技术用于马的生殖生理、疾病防控等方面的研究应用进行综述,并结合现代技术发展对未来运用前景做展望,为马的遗传育种研究和生产提供一定的参考依据。展开更多
基金the members of the Extending Station for Animal Husbandry and Veterinary Technology of Tianzhu Xizang Autonomous County for their help in sample collection and data acquisition.This research was supported by the Supercomputing Center of Lanzhou University and the Chakouyi horse conservation project from the Tianzhu Xizang Autonomous County Government([20]0097).
文摘Chakouyi(CKY)horses from the Qinghai-Xizang Plateau are well known for their unique lateral gaits and high-altitude adaptation,but genetic mechanisms underlying these phenotypes remain unclear.This study presents a comparison of 60 newly resequenced genomes of gaited CKY horses with 139 public genomes from 19 horse breeds.Population structure analyses(admixture,PCA,and neighbor-joining tree)reveal a close genetic relationship between CKY and other highland breeds(Tibetan and Chaidamu horses).Compared with other Chinese breeds,CKY horses present reduced nucleotide diversity(θπ)and lower inbreeding(FROHcoefficient),suggesting possible selective pressures.A key region on chromosome 23(Chr23:22.3-22.6 Mb)is associated with the lateral gaits and harbors a highly prevalent nonsense mutation(Chr 23:22,391,254 C>A,Ser301STOP)in the DMRT3 gene,with an 88%homozygosity rate,which is strongly correlated with the distinctive gait of CKY horses.Furthermore,selection signals reveal that the EPAS1 gene is related to high-altitude adaptation,and the CAT gene contributes to altitude resilience in CKY horses.These findings suggest that preserving genetic diversity is essential for maintaining the unique gaits and high-altitude adaptations of CKY horses.
文摘成簇规则间隔短回文重复序列相关蛋白9 (The clustered regularly interspaced short palindromic repeats/CRISPR associated protein 9, CRISPR/Cas9)基因编辑技术是目前探索动物基因功能的有效手段之一。CRISPR/Cas9基因编辑技术表现出对宿主细胞内失活和/或消除整合前病毒DNA的显著潜力,对创造优良性状和提高生产性能具有深远的意义。尽管CRISPR/Cas9基因编辑技术在模式动物上已取得诸多成果,但应用于马的基因编辑研究仍处于起步阶段。该文就CRISPR/Cas9基因编辑技术用于马的生殖生理、疾病防控等方面的研究应用进行综述,并结合现代技术发展对未来运用前景做展望,为马的遗传育种研究和生产提供一定的参考依据。
