The stem of Ginkgo biloba were cultured on MS,modified MS and DCR basal medium,supplemented with various concentration of hormones—NAA,ZT (zeatin) and two types of nurture-CH (casein hydrolysate) and Ade (adenine). T...The stem of Ginkgo biloba were cultured on MS,modified MS and DCR basal medium,supplemented with various concentration of hormones—NAA,ZT (zeatin) and two types of nurture-CH (casein hydrolysate) and Ade (adenine). The aim was to study the effects of various basal medium,different hormones and nurtures on the growth and development of Ginkgo biloba stem. The result showed that the modified MS with CH 500 mg·L -1 was the best medium for inducing the development of axillary buds;the medium with NAA,ZT and Ade promoted axillary buds to be callus and inhibited them developing into shoots. The shoot rate was 89.3%. After cultured for 40 days,the clustered buds were emerged from axillary buds. The detached buds from clustered buds were cultured on modified MS basal medium or supplemented with CH 500 mg·L -1 . The shoots grew well,and the reproduction coefficient was 3. The shoots from apical buds were also reproduced,and the reproduction coefficient was 4. The shoots were induced to root on MS+NAA 0.1 mg·L -1 medium,and the rooting rate was 33.3%.展开更多
Terpene lactone contents in different organs of Ginkgo biloba L. and leaf, stem and root of G. biloba seedlings treated with chlorocholine chloride were determined by GC. The results showed that total terpene lactone ...Terpene lactone contents in different organs of Ginkgo biloba L. and leaf, stem and root of G. biloba seedlings treated with chlorocholine chloride were determined by GC. The results showed that total terpene lactone contents in the order from high to low were bud (1.973 mg·g-1), leaf (1.753 mg·g-1), root, stem, and the terpene lactone content in phloem was higher than that in xylem. Leaf contained more bilobalide than root and stem. Chlorocholine chloride treatment significantly increased terpene lactone content of G. biloba seedlings. The possible way of terpene lactone biosynthesis was discussed.展开更多
文摘The stem of Ginkgo biloba were cultured on MS,modified MS and DCR basal medium,supplemented with various concentration of hormones—NAA,ZT (zeatin) and two types of nurture-CH (casein hydrolysate) and Ade (adenine). The aim was to study the effects of various basal medium,different hormones and nurtures on the growth and development of Ginkgo biloba stem. The result showed that the modified MS with CH 500 mg·L -1 was the best medium for inducing the development of axillary buds;the medium with NAA,ZT and Ade promoted axillary buds to be callus and inhibited them developing into shoots. The shoot rate was 89.3%. After cultured for 40 days,the clustered buds were emerged from axillary buds. The detached buds from clustered buds were cultured on modified MS basal medium or supplemented with CH 500 mg·L -1 . The shoots grew well,and the reproduction coefficient was 3. The shoots from apical buds were also reproduced,and the reproduction coefficient was 4. The shoots were induced to root on MS+NAA 0.1 mg·L -1 medium,and the rooting rate was 33.3%.
文摘Terpene lactone contents in different organs of Ginkgo biloba L. and leaf, stem and root of G. biloba seedlings treated with chlorocholine chloride were determined by GC. The results showed that total terpene lactone contents in the order from high to low were bud (1.973 mg·g-1), leaf (1.753 mg·g-1), root, stem, and the terpene lactone content in phloem was higher than that in xylem. Leaf contained more bilobalide than root and stem. Chlorocholine chloride treatment significantly increased terpene lactone content of G. biloba seedlings. The possible way of terpene lactone biosynthesis was discussed.
