Both genders of the dioecious gymnosperm Ginkgo bilob a have distinct practical production and application uses,so quick,accurate identification of males and females is important for early seedling breeding.To develop...Both genders of the dioecious gymnosperm Ginkgo bilob a have distinct practical production and application uses,so quick,accurate identification of males and females is important for early seedling breeding.To develop a fast method to identify the sexes,we used the Easy DNA extraction(EZ-D)method to extract DNA from leaves within1 min for use with the recombinase polymerase amplification-lateral flow dipstick(RPA-LFD)system and identify the sex.A portable nucleic acid detection card kit(PNADCK)was used for on-site analysis.This method facilitates rapid extraction of nucleic acids from a single and can accurately detect 100 pg/μL of G.biloba female genomic DNA within20 min at 39°C.The EZ-DRPA-LFD-PNADCK system enables precise on-site determination of G.bilob a leaf sex and is rapid,efficient,sensitive,and convenient,greatly enhancing productivity for G.biloba because seedlings with specific sex characteristics can be selected at an earlier stage,planting strategies can be optimized,and production efficiency improved.展开更多
根据其他植物PAL基因的DNA序列的保守区域,设计了一对简并引物,经PCR扩增,得到一条862 bp的特异性扩增带。将PCR扩增产物构建到T-载体并测序,获得了862 bp DNA序列。通过分析所得的862 bp DNA序列及其编码的氨基酸序列,与其他植物PAL基...根据其他植物PAL基因的DNA序列的保守区域,设计了一对简并引物,经PCR扩增,得到一条862 bp的特异性扩增带。将PCR扩增产物构建到T-载体并测序,获得了862 bp DNA序列。通过分析所得的862 bp DNA序列及其编码的氨基酸序列,与其他植物PAL基因的DNA序列和蛋白质序列分别进行比较,证实了所得序列为银杏PAL基因的部分序列。Gbpal1已经被Genebank收录,序列号为AY578145。Southern杂交结果表明银杏PAL是一个多基因家族。展开更多
基金supported by the National Natural Science Foundation of China(Grant 32471873)the Major Projects in Agricultural Biological Breeding(Grant 2023ZD04056)+3 种基金the National Key R&D Program of China(Grant 2023YFD1401304)the Natural Science Foundation of Jiangsu Province,China(Grants BK20221426,BK20231291)the China Postdoctoral Science Foundation project(Grant 2024M751426)the Postgraduate Research&Practice Innovation Program of Jiangsu Province(Grant KYCX241264)。
文摘Both genders of the dioecious gymnosperm Ginkgo bilob a have distinct practical production and application uses,so quick,accurate identification of males and females is important for early seedling breeding.To develop a fast method to identify the sexes,we used the Easy DNA extraction(EZ-D)method to extract DNA from leaves within1 min for use with the recombinase polymerase amplification-lateral flow dipstick(RPA-LFD)system and identify the sex.A portable nucleic acid detection card kit(PNADCK)was used for on-site analysis.This method facilitates rapid extraction of nucleic acids from a single and can accurately detect 100 pg/μL of G.biloba female genomic DNA within20 min at 39°C.The EZ-DRPA-LFD-PNADCK system enables precise on-site determination of G.bilob a leaf sex and is rapid,efficient,sensitive,and convenient,greatly enhancing productivity for G.biloba because seedlings with specific sex characteristics can be selected at an earlier stage,planting strategies can be optimized,and production efficiency improved.
文摘根据其他植物PAL基因的DNA序列的保守区域,设计了一对简并引物,经PCR扩增,得到一条862 bp的特异性扩增带。将PCR扩增产物构建到T-载体并测序,获得了862 bp DNA序列。通过分析所得的862 bp DNA序列及其编码的氨基酸序列,与其他植物PAL基因的DNA序列和蛋白质序列分别进行比较,证实了所得序列为银杏PAL基因的部分序列。Gbpal1已经被Genebank收录,序列号为AY578145。Southern杂交结果表明银杏PAL是一个多基因家族。
