The present investigation was carried out to investigate the effect of heat stress and revival on some antioxidative enzymes and metabolites in leaves of the wheat （Triticum aestivum L.） seedlings of heat susceptib...The present investigation was carried out to investigate the effect of heat stress and revival on some antioxidative enzymes and metabolites in leaves of the wheat （Triticum aestivum L.） seedlings of heat susceptible （cv. WH 147 and HS 277） and heat tolerant （cv. WH 1021 and HW 2045） cultivars. Seven days old seedlings grown at 25 ℃ were exposed to 40 ℃ for 6 h and these seedlings were again brought to 25 ℃. The observations were recorded in the leaves of control, stressed and revived seedlings on 2nd and 4th day of revival. For the selection ofthermo-tolerant cultivars, screening of the thirty-six cultivars was done based on wilting of primary leaf and values of chlorophyll fluorescence. The MDA （malondialdehyde） and H2O2 concentration in leaves of wheat seedlings increased at the high temperature. There was enhancement in the activities of antioxidative enzymes, viz. CAT （catalase）, POX （peroxidase）, GR （glutathione reductase） and APX （ascorbate peroxidase） in leaves of the tolerant and susceptible cultivars under heat stress, however, higher percent increase was observed in tolerant cultivars. Heat stress increased the SOD （superoxide dismutase） activity in tolerant cultivars but activity declined in susceptible cultivars. On revival, the activities of the CAT, POX and GR declined in comparison to stressed seedlings but remained higher as compared to control. Ascorbate peroxidase activity remained higher on 2nd day and 4th day of revival in all the cultivars.展开更多
文摘The present investigation was carried out to investigate the effect of heat stress and revival on some antioxidative enzymes and metabolites in leaves of the wheat （Triticum aestivum L.） seedlings of heat susceptible （cv. WH 147 and HS 277） and heat tolerant （cv. WH 1021 and HW 2045） cultivars. Seven days old seedlings grown at 25 ℃ were exposed to 40 ℃ for 6 h and these seedlings were again brought to 25 ℃. The observations were recorded in the leaves of control, stressed and revived seedlings on 2nd and 4th day of revival. For the selection ofthermo-tolerant cultivars, screening of the thirty-six cultivars was done based on wilting of primary leaf and values of chlorophyll fluorescence. The MDA （malondialdehyde） and H2O2 concentration in leaves of wheat seedlings increased at the high temperature. There was enhancement in the activities of antioxidative enzymes, viz. CAT （catalase）, POX （peroxidase）, GR （glutathione reductase） and APX （ascorbate peroxidase） in leaves of the tolerant and susceptible cultivars under heat stress, however, higher percent increase was observed in tolerant cultivars. Heat stress increased the SOD （superoxide dismutase） activity in tolerant cultivars but activity declined in susceptible cultivars. On revival, the activities of the CAT, POX and GR declined in comparison to stressed seedlings but remained higher as compared to control. Ascorbate peroxidase activity remained higher on 2nd day and 4th day of revival in all the cultivars.
