An oligonucleotide primer set (PaF/PaR) was designed for polymerase chain reaction (PCR) amplification of an approximately 1. 2kb fragment DNA from Paulownia Witches’ broom(PaWB) MLO on the basis of 16S rRNA sequence...An oligonucleotide primer set (PaF/PaR) was designed for polymerase chain reaction (PCR) amplification of an approximately 1. 2kb fragment DNA from Paulownia Witches’ broom(PaWB) MLO on the basis of 16S rRNA sequence from O-MLO. Amplification of a 1. 2kb DNA fragment from PaWB-MLO-infected plants DNA in total nucleic acid extrats of the host plant Paulownlh spp., but no l. 2kb DNA fragment was observed when healthy DNA was used as a template. The study showed that the PCR method was able todetect PaWB-MLO in as little as 9. 4pg of total DNA from infected Paulernia tissue culture plantlets.展开更多
文摘An oligonucleotide primer set (PaF/PaR) was designed for polymerase chain reaction (PCR) amplification of an approximately 1. 2kb fragment DNA from Paulownia Witches’ broom(PaWB) MLO on the basis of 16S rRNA sequence from O-MLO. Amplification of a 1. 2kb DNA fragment from PaWB-MLO-infected plants DNA in total nucleic acid extrats of the host plant Paulownlh spp., but no l. 2kb DNA fragment was observed when healthy DNA was used as a template. The study showed that the PCR method was able todetect PaWB-MLO in as little as 9. 4pg of total DNA from infected Paulernia tissue culture plantlets.
