Newly grown branch buds on node of Dendrocalamus latiflorus were used as explants to induce buds in MS, 1/2 MS, and N_ 6 media with different hormone combinations, and tube seedlings were obtained. In order to improve...Newly grown branch buds on node of Dendrocalamus latiflorus were used as explants to induce buds in MS, 1/2 MS, and N_ 6 media with different hormone combinations, and tube seedlings were obtained. In order to improve its reproductive coefficient, survival rate and rooting morphology, four aspects were tested: the number of the inoculated seedlings per cluster, multiplication culture, rooting culture and field transplanting. The results showed that the optimum medium for primary culture was MS+BA2.0 mg5L+{-1}+IBA 0.5 mg5L+{-1}; the reproductive coefficient reached 7.31 with sturdy seedlings during multiplication and rooting culture, if four seedlings were inoculated per cluster in MS+ BA2.0 mg5L+{-1} + IBA0.3 mg5L+{-1} medium; the optimal medium for rooting was 1/2MS+ IBA4.0 mg5L+{-1}, with survival rate as high as 95%. The rooted seedlings were transplanted in the medium consisting of perlite+ earth+ cinders ash (1∶1∶1); After 3 to 7 days of hardening in the bottle and then growing for 10 to 15 days, 90% of the seedlings were survived. Through the above method, more than 2 million bamboo seedlings have been propagated so far.展开更多
文摘Newly grown branch buds on node of Dendrocalamus latiflorus were used as explants to induce buds in MS, 1/2 MS, and N_ 6 media with different hormone combinations, and tube seedlings were obtained. In order to improve its reproductive coefficient, survival rate and rooting morphology, four aspects were tested: the number of the inoculated seedlings per cluster, multiplication culture, rooting culture and field transplanting. The results showed that the optimum medium for primary culture was MS+BA2.0 mg5L+{-1}+IBA 0.5 mg5L+{-1}; the reproductive coefficient reached 7.31 with sturdy seedlings during multiplication and rooting culture, if four seedlings were inoculated per cluster in MS+ BA2.0 mg5L+{-1} + IBA0.3 mg5L+{-1} medium; the optimal medium for rooting was 1/2MS+ IBA4.0 mg5L+{-1}, with survival rate as high as 95%. The rooted seedlings were transplanted in the medium consisting of perlite+ earth+ cinders ash (1∶1∶1); After 3 to 7 days of hardening in the bottle and then growing for 10 to 15 days, 90% of the seedlings were survived. Through the above method, more than 2 million bamboo seedlings have been propagated so far.
