In order to elucidate the mechanism of the seed germination in Cistanche deserticola,we investigated the effects of stratification treatment of high/low temperature on seed germination of C.deserticola using the tissu...In order to elucidate the mechanism of the seed germination in Cistanche deserticola,we investigated the effects of stratification treatment of high/low temperature on seed germination of C.deserticola using the tissue culture technique.The effects of high/low temperature stratification and plant hormones on seed germination rate of C.deserticola were investigated using the orthogonal experiment design.Results showed that the seed germination rate was 6.7% at 50 ℃ for 1 h and 1 week at（4 ℃） twice repeated stratification treatment for the seeds,and the germination took 60 d.To the contrast,the seed germination rate was 17.8% at 50 ℃ for 1 h and 1 week at 4 ℃ twice repeated stratification treatment combined with 1.4 μmol·L-16-BA,（2.9 μmol·L）-1 GA3 and 5.8 μmol·L-1 IBA treatment,and the germination needed only 30 d.GA3 was a key plant hormone to the seed germination to promote the germination rate at 50 ℃ and 1 w at 4 ℃.Two repeated stratification treatments and exogenous plant hormone were the essential conditions for the seed germination after being excised from host.展开更多
文摘In order to elucidate the mechanism of the seed germination in Cistanche deserticola,we investigated the effects of stratification treatment of high/low temperature on seed germination of C.deserticola using the tissue culture technique.The effects of high/low temperature stratification and plant hormones on seed germination rate of C.deserticola were investigated using the orthogonal experiment design.Results showed that the seed germination rate was 6.7% at 50 ℃ for 1 h and 1 week at（4 ℃） twice repeated stratification treatment for the seeds,and the germination took 60 d.To the contrast,the seed germination rate was 17.8% at 50 ℃ for 1 h and 1 week at 4 ℃ twice repeated stratification treatment combined with 1.4 μmol·L-16-BA,（2.9 μmol·L）-1 GA3 and 5.8 μmol·L-1 IBA treatment,and the germination needed only 30 d.GA3 was a key plant hormone to the seed germination to promote the germination rate at 50 ℃ and 1 w at 4 ℃.Two repeated stratification treatments and exogenous plant hormone were the essential conditions for the seed germination after being excised from host.
