The leaf anatomic structure of %Zizyphus jujuba% var. %spinosa% from seashore to western arid area (near 37° N latitude) of China were investigated with both a light microscope and a scanning electron microscope....The leaf anatomic structure of %Zizyphus jujuba% var. %spinosa% from seashore to western arid area (near 37° N latitude) of China were investigated with both a light microscope and a scanning electron microscope. The results showed that all provenances shared the following common features. The distribution of stomata was generally uniform on the abaxial surface, and the type of stomatal apparatus was mainly anomocytic. The upper epidermis was formed by one layer of cells with tight, regular arrangement, and the lower epidermis was also formed by one layer of cells, and the type of anticlinal cell wall on both the upper and on the lower epidermis was straight. In the contrast, there were some differences in the anatomic characteristics among the provenances. With the decrease of water content in the habitats, (1) the stomatas became smaller gradually, and the density of stomata increase gradually; (2) the cells of both the upper and the lower epidermis became smaller gradually; (3) thickness of the upper and the lower cuticle, as well as the thickness of leaf, increased gradually; (4) bifacial leaf changed to isobilateral leaf; (5) the proportion of palisade tissue increased gradually, whereas the spongy tissue decreased; (6) diameter of midrib vessels and of midrib vascular bundles increased gradually; (7) the epidermis ornament changed in an order of smoothness, strip, reticulation, and mountain-like that represented the leaf structural characteristics of xerophytes. The results also showed that the sequence of the drought resistance delicacy was thickness of upper cuticle>density of stomata in proximal surface, size of stomata in proximal surface (length/width), thickness of lower cuticle, thickness of palisade, and thickness of spongy>average length of stomata, average length/width of upper epidermis cells, thickness of leaf, value of CTR, thickness of upper epiderm cell, thickness of lower epidermic cell, layers of palisade>diameter of midrib vascular bundle, average width of stomata, the area of stomata in proximal surface, average length of lower epidermis cells, and average length/width of lower epidermis cells>average length/width of palisade tissue cell.展开更多
In order to discuss the relationship among the wild herbaceous peonies native in China according to microscopic characteristics, the pollen grains of four wild herbaceous peonies were examined under scanning electron ...In order to discuss the relationship among the wild herbaceous peonies native in China according to microscopic characteristics, the pollen grains of four wild herbaceous peonies were examined under scanning electron microscope (SEM), and the data acquired were used in clustering analysis. The result with SEM showed distinctly distinguishing extine surface pattern of taxonomic importance among the four species: reticulate pattern for Paeonia lactiflora; micro-foveolate, regulate and regulate-foveolate pattern for P. veitchii ; regulate and subcerebro-reticulate pattern for P. obovata ; regulate pattern for P. anomala var. intermedia . The result with clustering analysis suggested the close relationship between P. veitchii and P. anomala var. intermedia for their Euclidean distance was only 2.51, and the alienation between P. lactiflora and the other three species for their Euclidean distances were all more than 5.00. In addition, we deduced P. obovata may be the linkage between subsect Foliolatae and subsect Dissectifoliae for its pollen morphology was appreciably inclined to P. veitchii and P. anomala var. intermedia.展开更多
文摘The leaf anatomic structure of %Zizyphus jujuba% var. %spinosa% from seashore to western arid area (near 37° N latitude) of China were investigated with both a light microscope and a scanning electron microscope. The results showed that all provenances shared the following common features. The distribution of stomata was generally uniform on the abaxial surface, and the type of stomatal apparatus was mainly anomocytic. The upper epidermis was formed by one layer of cells with tight, regular arrangement, and the lower epidermis was also formed by one layer of cells, and the type of anticlinal cell wall on both the upper and on the lower epidermis was straight. In the contrast, there were some differences in the anatomic characteristics among the provenances. With the decrease of water content in the habitats, (1) the stomatas became smaller gradually, and the density of stomata increase gradually; (2) the cells of both the upper and the lower epidermis became smaller gradually; (3) thickness of the upper and the lower cuticle, as well as the thickness of leaf, increased gradually; (4) bifacial leaf changed to isobilateral leaf; (5) the proportion of palisade tissue increased gradually, whereas the spongy tissue decreased; (6) diameter of midrib vessels and of midrib vascular bundles increased gradually; (7) the epidermis ornament changed in an order of smoothness, strip, reticulation, and mountain-like that represented the leaf structural characteristics of xerophytes. The results also showed that the sequence of the drought resistance delicacy was thickness of upper cuticle>density of stomata in proximal surface, size of stomata in proximal surface (length/width), thickness of lower cuticle, thickness of palisade, and thickness of spongy>average length of stomata, average length/width of upper epidermis cells, thickness of leaf, value of CTR, thickness of upper epiderm cell, thickness of lower epidermic cell, layers of palisade>diameter of midrib vascular bundle, average width of stomata, the area of stomata in proximal surface, average length of lower epidermis cells, and average length/width of lower epidermis cells>average length/width of palisade tissue cell.
文摘In order to discuss the relationship among the wild herbaceous peonies native in China according to microscopic characteristics, the pollen grains of four wild herbaceous peonies were examined under scanning electron microscope (SEM), and the data acquired were used in clustering analysis. The result with SEM showed distinctly distinguishing extine surface pattern of taxonomic importance among the four species: reticulate pattern for Paeonia lactiflora; micro-foveolate, regulate and regulate-foveolate pattern for P. veitchii ; regulate and subcerebro-reticulate pattern for P. obovata ; regulate pattern for P. anomala var. intermedia . The result with clustering analysis suggested the close relationship between P. veitchii and P. anomala var. intermedia for their Euclidean distance was only 2.51, and the alienation between P. lactiflora and the other three species for their Euclidean distances were all more than 5.00. In addition, we deduced P. obovata may be the linkage between subsect Foliolatae and subsect Dissectifoliae for its pollen morphology was appreciably inclined to P. veitchii and P. anomala var. intermedia.
