SWEET转运蛋白通过交替转运机制介导单糖和二糖双向跨膜运输,在植物的生长发育过程中糖的分配和利用起关键作用。本研究基于水仙(Narcissus tazetta var. chinensis)转录组分析获得的9条在花器官中表达量相对较高的9个SWEEET基因,这些Nt...SWEET转运蛋白通过交替转运机制介导单糖和二糖双向跨膜运输,在植物的生长发育过程中糖的分配和利用起关键作用。本研究基于水仙(Narcissus tazetta var. chinensis)转录组分析获得的9条在花器官中表达量相对较高的9个SWEEET基因,这些NtSWEETs在SWEET家族的4个分支中皆有分布。在水仙开花过程中,NtSWEET1a、NtSWEET1b和NtSWEET4b基因在花被片中的表达水平随着花朵的开放表达量逐渐上升,其他基因则无明显规律;在花芽分化过程中,NtSWEET1a、NtSWEET1b和NtSWEET13既有较高的表达水平,又在鳞茎中各组织里的表达水平随着花芽分化而逐渐上升;在水仙鳞茎膨大期间,NtSWEET1a在鳞茎膨大期间保持较高的表达量,且随着鳞茎的膨大表达水平上升。进一步利用酵母功能互补实验分析这些SWEET蛋白对葡萄糖、半乳糖、甘露糖、果糖、蔗糖的转运功能,发现NtSWEET1a可以转运葡萄糖和半乳糖,暗示NtSWEET1a在开花、花芽分化及鳞茎膨大过程中参与了糖的转运,特别是葡萄糖和半乳糖的转运有重要作用。展开更多
A new gene,named as NTZDS1(GenBank accession number:EU138882),was cloned from the alabastrum of Narcissus tazetta var.chinensis through RT-PCR.The sequence consists of 1 899 bp,has an open reading frame of 1 725 bp en...A new gene,named as NTZDS1(GenBank accession number:EU138882),was cloned from the alabastrum of Narcissus tazetta var.chinensis through RT-PCR.The sequence consists of 1 899 bp,has an open reading frame of 1 725 bp encoding a polypeptide of 574 amino acids.Homology analysis showed that the deduced NTZDS1 protein was highly homologous to other ZDS proteins from different species.Phylogenetic analysis indicated that NTZDS1 was more related to ZDS of Narcissus pseudonarcissus(O 49901).NTZDS1 gene was detected in flowers,leaves and roots except scales,and expression level was especially high in flowers.Within open flowers,level was the highest in petals and higher in corana while lower in stamens and pistils.展开更多
文摘SWEET转运蛋白通过交替转运机制介导单糖和二糖双向跨膜运输,在植物的生长发育过程中糖的分配和利用起关键作用。本研究基于水仙(Narcissus tazetta var. chinensis)转录组分析获得的9条在花器官中表达量相对较高的9个SWEEET基因,这些NtSWEETs在SWEET家族的4个分支中皆有分布。在水仙开花过程中,NtSWEET1a、NtSWEET1b和NtSWEET4b基因在花被片中的表达水平随着花朵的开放表达量逐渐上升,其他基因则无明显规律;在花芽分化过程中,NtSWEET1a、NtSWEET1b和NtSWEET13既有较高的表达水平,又在鳞茎中各组织里的表达水平随着花芽分化而逐渐上升;在水仙鳞茎膨大期间,NtSWEET1a在鳞茎膨大期间保持较高的表达量,且随着鳞茎的膨大表达水平上升。进一步利用酵母功能互补实验分析这些SWEET蛋白对葡萄糖、半乳糖、甘露糖、果糖、蔗糖的转运功能,发现NtSWEET1a可以转运葡萄糖和半乳糖,暗示NtSWEET1a在开花、花芽分化及鳞茎膨大过程中参与了糖的转运,特别是葡萄糖和半乳糖的转运有重要作用。
文摘A new gene,named as NTZDS1(GenBank accession number:EU138882),was cloned from the alabastrum of Narcissus tazetta var.chinensis through RT-PCR.The sequence consists of 1 899 bp,has an open reading frame of 1 725 bp encoding a polypeptide of 574 amino acids.Homology analysis showed that the deduced NTZDS1 protein was highly homologous to other ZDS proteins from different species.Phylogenetic analysis indicated that NTZDS1 was more related to ZDS of Narcissus pseudonarcissus(O 49901).NTZDS1 gene was detected in flowers,leaves and roots except scales,and expression level was especially high in flowers.Within open flowers,level was the highest in petals and higher in corana while lower in stamens and pistils.
