Ascorbic acid, also referred to as vitamin C(Vc), is an important nutrient found in fruits and vegetables that promotes produce quality and human health. Rosa roxburghii is an underutilized natural fruit that contains...Ascorbic acid, also referred to as vitamin C(Vc), is an important nutrient found in fruits and vegetables that promotes produce quality and human health. Rosa roxburghii is an underutilized natural fruit that contains very high levels of Vc. However, the Vc content of R. roxburghii varies considerably during plant development and ripening. To better understand the molecular mechanisms that underlie fluctuations in Vc content of R. roxburghii fruit at different developmental stages, we performed transcriptomic and metabolomic analyses and identified two significant gene networks/modules and 168 transcription factors directly involved in Vc synthesis. Promoter analysis of two core genes involved in Vc synthesis, RrGGP and RrGalUR, revealed the presence of a retroviral long terminal repeat(LTR) insert in the RrGalUR promoter. Using yeast one-hybrid and dual-luciferase assays, we demonstrated that the transcription factors RrHY5H and RrZIP9 bind to the promoter of RrGGP to promote its expression. RrZIP6 and RrWRKY4 bind to the LTR in the RrGalUR promoter to promote its expression. Our results reveal a molecular mechanism that controls Vc synthesis and accumulation in R. roxburghii fruit.展开更多
扁桃(Amygdalus communis L.)属蔷薇科李亚科桃属扁桃亚属植物,为世界著名的干果树种,起源于中亚细亚,约6000年的栽培历史,大面积栽培始于19世纪后期。目前扁桃的引种栽培遍及美国、西班牙、希腊、意大利、土耳其等32个国家和地区,其中...扁桃(Amygdalus communis L.)属蔷薇科李亚科桃属扁桃亚属植物,为世界著名的干果树种,起源于中亚细亚,约6000年的栽培历史,大面积栽培始于19世纪后期。目前扁桃的引种栽培遍及美国、西班牙、希腊、意大利、土耳其等32个国家和地区,其中美国的栽培面积及产量均居世界之首。在我国,栽培扁桃的分布和产区位于新疆的南部,目前的栽培面积约10000hm^2。2001年世界扁桃总产量为610769t,其中美国的年总产量为397000t,其产量的75％出口到40多个国家,创汇5.87亿美元。综合有关文献介绍了世界扁桃的分布、产业发展和我国扁桃的引种、栽培概况及国内外扁桃的研究进展情况。展开更多
基金supported in part by the Priority Academic Program Development of Jiangsu Higher Education Institutions and the State Key Laboratory of Crop Genetics and Germplasm Enhancement (Grant No. ZW201813)supported by the high-performance computing platform at the Bioinformatics Center of Nanjing Agricultural University。
文摘Ascorbic acid, also referred to as vitamin C(Vc), is an important nutrient found in fruits and vegetables that promotes produce quality and human health. Rosa roxburghii is an underutilized natural fruit that contains very high levels of Vc. However, the Vc content of R. roxburghii varies considerably during plant development and ripening. To better understand the molecular mechanisms that underlie fluctuations in Vc content of R. roxburghii fruit at different developmental stages, we performed transcriptomic and metabolomic analyses and identified two significant gene networks/modules and 168 transcription factors directly involved in Vc synthesis. Promoter analysis of two core genes involved in Vc synthesis, RrGGP and RrGalUR, revealed the presence of a retroviral long terminal repeat(LTR) insert in the RrGalUR promoter. Using yeast one-hybrid and dual-luciferase assays, we demonstrated that the transcription factors RrHY5H and RrZIP9 bind to the promoter of RrGGP to promote its expression. RrZIP6 and RrWRKY4 bind to the LTR in the RrGalUR promoter to promote its expression. Our results reveal a molecular mechanism that controls Vc synthesis and accumulation in R. roxburghii fruit.
文摘扁桃(Amygdalus communis L.)属蔷薇科李亚科桃属扁桃亚属植物,为世界著名的干果树种,起源于中亚细亚,约6000年的栽培历史,大面积栽培始于19世纪后期。目前扁桃的引种栽培遍及美国、西班牙、希腊、意大利、土耳其等32个国家和地区,其中美国的栽培面积及产量均居世界之首。在我国,栽培扁桃的分布和产区位于新疆的南部,目前的栽培面积约10000hm^2。2001年世界扁桃总产量为610769t,其中美国的年总产量为397000t,其产量的75％出口到40多个国家,创汇5.87亿美元。综合有关文献介绍了世界扁桃的分布、产业发展和我国扁桃的引种、栽培概况及国内外扁桃的研究进展情况。
