本研究以‘毕克齐’和‘大和长芋’山药块茎为试验材料,通过RACE技术克隆山药BAM1基因,并进行生物信息学分析与亚细胞定位;分析BAM1在山药块茎不同发育时期的表达模式;筛选其互作蛋白,并研究其在低温、干旱和盐胁迫下的表达特征。结果表...本研究以‘毕克齐’和‘大和长芋’山药块茎为试验材料,通过RACE技术克隆山药BAM1基因,并进行生物信息学分析与亚细胞定位;分析BAM1在山药块茎不同发育时期的表达模式;筛选其互作蛋白,并研究其在低温、干旱和盐胁迫下的表达特征。结果表明,山药BAM1基因全长2066 bp,有BAMs家族基因的保守结构域Glyco_hydro_14,与几内亚薯蓣亲缘关系较近;该基因编码蛋白定位于细胞膜;在2个山药品种中,BAM1基因的表达量均在块茎膨大的前期和后期处于较高水平;通过酵母双杂交筛选出17个与其互作的蛋白,表达分析结果表明BAM1基因及其互作基因GEM-like Protein 5、TIFY10a-like和CXXS1响应植物的低温、干旱和盐胁迫。展开更多
The yam Dioscorea alata L.is widely cultivated globally.Purple-fleshed varieties of this important crop have enhanced market value due to their high anthocyanin contents,but how anthocyanin biosynthesis in D.alata tub...The yam Dioscorea alata L.is widely cultivated globally.Purple-fleshed varieties of this important crop have enhanced market value due to their high anthocyanin contents,but how anthocyanin biosynthesis in D.alata tubers is regulated remains poorly understood.In this study,we identified and functionally validated key transcription factors that regulate anthocyanin biosynthesis based on a comparative transcriptome and metabolome analysis of three D.alata cultivars with different colored tubers(dark purple,light purple,and white).The anthocyanin glycoside cyanidin-3-O-(2′′-O-glucosyl)glucoside was abundant during early tuber development,and we determined that its accumulation is regulated in opposite manners by two R2R3-MYB transcription factors:DaMYB75 and DaMYB56.Yeast two-hybrid and bimolecular fluorescence complementation assays in Nicotiana benthamiana and co-expression assays in D.alata demonstrated that DaMYB75 promotes anthocyanin biosynthesis by specifically activating the promoter of the late anthocyanin biosynthesis gene DaANS and enhancing its expression through an interaction with DabHLH72.By contrast,DaMYB56 is a negative regulator of anthocyanin biosynthesis that binds to the DaANS promoter together with DabHLH72.Furthermore,the methylation levels of the DaMYB75 promoter were significantly lower in purple tubers than in white tubers.These findings shed light on the regulation of anthocyanin biosynthesis by MYBs and provide the basis for genetically improving anthocyanin content in D.alata.展开更多
Yam(Dioscorea opposita)is an important economic crop,and the development of its the cultivation tecchnology is of great significance for increasing yield,improving quality,and reducing the occurrence of pests and dise...Yam(Dioscorea opposita)is an important economic crop,and the development of its the cultivation tecchnology is of great significance for increasing yield,improving quality,and reducing the occurrence of pests and diseases.With the progression of agricultural technology and changes in market demands,the cultivation tecchnology of yam is constantly innovating and improving.This paper reviewed recent research advances in yam cultivation tecchnology,covering aspects such as soil improvement,variety selection,technological innovations,green pest control,and harvesting and storage.The goal is to provide references for the sustainable development of the yam industry.展开更多
文摘本研究以‘毕克齐’和‘大和长芋’山药块茎为试验材料,通过RACE技术克隆山药BAM1基因,并进行生物信息学分析与亚细胞定位;分析BAM1在山药块茎不同发育时期的表达模式;筛选其互作蛋白,并研究其在低温、干旱和盐胁迫下的表达特征。结果表明,山药BAM1基因全长2066 bp,有BAMs家族基因的保守结构域Glyco_hydro_14,与几内亚薯蓣亲缘关系较近;该基因编码蛋白定位于细胞膜;在2个山药品种中,BAM1基因的表达量均在块茎膨大的前期和后期处于较高水平;通过酵母双杂交筛选出17个与其互作的蛋白,表达分析结果表明BAM1基因及其互作基因GEM-like Protein 5、TIFY10a-like和CXXS1响应植物的低温、干旱和盐胁迫。
基金supported by the National Natural Science Foundation of China(32460767)Jiangxi Provincial Key Research and Development Program(20232BBF60007)Jiangxi Provincial Natural Science Foundation(20224BAB205024).
文摘The yam Dioscorea alata L.is widely cultivated globally.Purple-fleshed varieties of this important crop have enhanced market value due to their high anthocyanin contents,but how anthocyanin biosynthesis in D.alata tubers is regulated remains poorly understood.In this study,we identified and functionally validated key transcription factors that regulate anthocyanin biosynthesis based on a comparative transcriptome and metabolome analysis of three D.alata cultivars with different colored tubers(dark purple,light purple,and white).The anthocyanin glycoside cyanidin-3-O-(2′′-O-glucosyl)glucoside was abundant during early tuber development,and we determined that its accumulation is regulated in opposite manners by two R2R3-MYB transcription factors:DaMYB75 and DaMYB56.Yeast two-hybrid and bimolecular fluorescence complementation assays in Nicotiana benthamiana and co-expression assays in D.alata demonstrated that DaMYB75 promotes anthocyanin biosynthesis by specifically activating the promoter of the late anthocyanin biosynthesis gene DaANS and enhancing its expression through an interaction with DabHLH72.By contrast,DaMYB56 is a negative regulator of anthocyanin biosynthesis that binds to the DaANS promoter together with DabHLH72.Furthermore,the methylation levels of the DaMYB75 promoter were significantly lower in purple tubers than in white tubers.These findings shed light on the regulation of anthocyanin biosynthesis by MYBs and provide the basis for genetically improving anthocyanin content in D.alata.
基金Supported by Shandong Provincial Key Research and Development Program(2023TZXD034)2024 National Undergraduate Innovation Training Program(202410061062).
文摘Yam(Dioscorea opposita)is an important economic crop,and the development of its the cultivation tecchnology is of great significance for increasing yield,improving quality,and reducing the occurrence of pests and diseases.With the progression of agricultural technology and changes in market demands,the cultivation tecchnology of yam is constantly innovating and improving.This paper reviewed recent research advances in yam cultivation tecchnology,covering aspects such as soil improvement,variety selection,technological innovations,green pest control,and harvesting and storage.The goal is to provide references for the sustainable development of the yam industry.
