人参属药用植物在栽培过程中存在连作障碍现象,而化感自毒作用是造成其连作障碍的关键因素之一。目前,化感物质种类的筛选和化感作用机理是植物生理生态研究的重点方向。本文通过搜索Web of Science数据库、提炼LetPub智库资源整理了人...人参属药用植物在栽培过程中存在连作障碍现象,而化感自毒作用是造成其连作障碍的关键因素之一。目前,化感物质种类的筛选和化感作用机理是植物生理生态研究的重点方向。本文通过搜索Web of Science数据库、提炼LetPub智库资源整理了人参属药用植物化感作用的文献,梳理和总结了化感物质种类及化感作用机制。人参属化感物质主要为酚酸类和皂苷类,剖析其作用机制主要是通过影响植物生长发育、影响细胞膜结构、功能及基因表达等方面,本研究提出在深入探究化感作用机制的同时,应关注人参植物与其他植物种间化感物质互惠作用,基于此互惠作用的人参栽培模式的优化,以及如何利用人参化感物质抑制病虫草害等未来发展方向。展开更多
Panax Ginseng(2n=48)represents a quintessential resource in traditional Chinese medicine,renowned for its outstanding medicinal and economic benefits(Choi,2008).But the late start in analyzing the ginseng genome and t...Panax Ginseng(2n=48)represents a quintessential resource in traditional Chinese medicine,renowned for its outstanding medicinal and economic benefits(Choi,2008).But the late start in analyzing the ginseng genome and the poorly developed genetic transformation system still impede the study of ginseng gene function and the application of molecular breeding.Transient transformation has the advantages of high efficiency,low cost,and short cycle while laying the foundation for stable genetic transformation(Chen et al.,2021).In the plant transformation process,the cell wall prevents exogenous DNA or protein entry,significantly reducing the efficiency of the transformation.Protoplasts,as exposed cells wrapped by the plasma membrane,are more likely to absorb exogenous DNA,RNA,and protein.Transgenic systems of protoplasts have been established in several species and applied in many fields,such as gene function research(Gou et al.,2020),gene editing(Yang et al.,2023),and physiological or molecular mechanism research(Aoyagi,2011).For instance,Oryza sativa protoplasts were employed to screen genes involved in rice defense signaling pathways through fluorescent reporter systems,with BiFC employed to verified inter-protein interactions(He et al.,2016).A study transformed Cannabis sativa L.protoplasts with the plasmids carrying GFP and RFP genes,evaluated the efficiency under different transformation conditions by flow cytometry,and verified the induction of synthetic DR5 promoter by IAA based on the constructed system(Beard et al.,2021).展开更多
文摘人参属药用植物在栽培过程中存在连作障碍现象,而化感自毒作用是造成其连作障碍的关键因素之一。目前,化感物质种类的筛选和化感作用机理是植物生理生态研究的重点方向。本文通过搜索Web of Science数据库、提炼LetPub智库资源整理了人参属药用植物化感作用的文献,梳理和总结了化感物质种类及化感作用机制。人参属化感物质主要为酚酸类和皂苷类,剖析其作用机制主要是通过影响植物生长发育、影响细胞膜结构、功能及基因表达等方面,本研究提出在深入探究化感作用机制的同时,应关注人参植物与其他植物种间化感物质互惠作用,基于此互惠作用的人参栽培模式的优化,以及如何利用人参化感物质抑制病虫草害等未来发展方向。
基金supported by the Genetic analysis of important quality and traits of ginseng and basic research on molecular design breeding(Grant No.U21A20405)。
文摘Panax Ginseng(2n=48)represents a quintessential resource in traditional Chinese medicine,renowned for its outstanding medicinal and economic benefits(Choi,2008).But the late start in analyzing the ginseng genome and the poorly developed genetic transformation system still impede the study of ginseng gene function and the application of molecular breeding.Transient transformation has the advantages of high efficiency,low cost,and short cycle while laying the foundation for stable genetic transformation(Chen et al.,2021).In the plant transformation process,the cell wall prevents exogenous DNA or protein entry,significantly reducing the efficiency of the transformation.Protoplasts,as exposed cells wrapped by the plasma membrane,are more likely to absorb exogenous DNA,RNA,and protein.Transgenic systems of protoplasts have been established in several species and applied in many fields,such as gene function research(Gou et al.,2020),gene editing(Yang et al.,2023),and physiological or molecular mechanism research(Aoyagi,2011).For instance,Oryza sativa protoplasts were employed to screen genes involved in rice defense signaling pathways through fluorescent reporter systems,with BiFC employed to verified inter-protein interactions(He et al.,2016).A study transformed Cannabis sativa L.protoplasts with the plasmids carrying GFP and RFP genes,evaluated the efficiency under different transformation conditions by flow cytometry,and verified the induction of synthetic DR5 promoter by IAA based on the constructed system(Beard et al.,2021).
