Bulked segregant analysis was employed to construct two mixed DNA pools to screen the RAPd marker linked with the fertilityrestoring gene（\%Rf\-i）\% of upland cotton. A total of 425 arbitrary 10mer oligonucleotide p...Bulked segregant analysis was employed to construct two mixed DNA pools to screen the RAPd marker linked with the fertilityrestoring gene（\%Rf\-i）\% of upland cotton. A total of 425 arbitrary 10mer oligonucleotide primers were screened on two DNA pools, bulked male fertile and sterile DNAs isolated from BC\-3 segregating population of （06132R×Simian No. 3）. Three primers produced repeatable polymorphisms between the paired bulks and their parents. DNA was extracted and amplified with these three primers for 92 plants of （Zhong 12A1×06132R）F\-2. Based on the male fertility scoring and RAPD amplification, it is found that one RAPD marker fragment designated OPV15 300 was linked with the fertilityrestoring gene （R f \-1） with a recombination value of 13.0±2.57%.展开更多
文摘Bulked segregant analysis was employed to construct two mixed DNA pools to screen the RAPd marker linked with the fertilityrestoring gene（\%Rf\-i）\% of upland cotton. A total of 425 arbitrary 10mer oligonucleotide primers were screened on two DNA pools, bulked male fertile and sterile DNAs isolated from BC\-3 segregating population of （06132R×Simian No. 3）. Three primers produced repeatable polymorphisms between the paired bulks and their parents. DNA was extracted and amplified with these three primers for 92 plants of （Zhong 12A1×06132R）F\-2. Based on the male fertility scoring and RAPD amplification, it is found that one RAPD marker fragment designated OPV15 300 was linked with the fertilityrestoring gene （R f \-1） with a recombination value of 13.0±2.57%.
