The synthesis of a new type of X-ray-sensitive compound ＂di-（1-hydroxylundecyl） diselenide＂ and its application in the preparation of a new type of liposome with X-ray sensitivity was reported.This new liposome wa...The synthesis of a new type of X-ray-sensitive compound ＂di-（1-hydroxylundecyl） diselenide＂ and its application in the preparation of a new type of liposome with X-ray sensitivity was reported.This new liposome was synthesized to encapsulate doxorubicin hydrochloride（Dox）,with its physical and chemical properties,stability,and radiation sensitivity determined.Based on the pH-gradient method,liposomal Dox was prepared via ultrasonic emulsification and then purified on a Sephadex G50 mini-column.UV spectrophotometry and liquid chromatography were used to detect the encapsulation efficiency and radiation sensitivity of the Dox-loaded liposome.The results show that through changes in release rate,this liposome shows a relative radiosensitivity.In terms of radiation sensitivity,the drug leak rate of the X-ray-sensitive Dox-loaded liposome increased gradually and peaked at 65.4% under the X-ray radiation of a dose of 10 Gy or more than 10 Gy,which is significantly different from that of ordinary liposomes.Meanwhile,X-ray-sensitive Dox-loaded liposome has a good dispersion stability,with an average particle size of approximate 120 nm.The efficiency of this liposome encapsulating Dox was 75.84%,slightly lower than that of ordinary liposomes.The X-ray-sensitive Dox-loaded liposome exhibited suspension stability within 30 d of storage at 4 °C,without visible precipitation.Di-（1-hydroxylundecyl） diselenide is safe and noncytotoxic and compared with those of synthetic phospholipids its synthesis is low cost and does not require complex conditions.展开更多
Herbage seeds of legume and grass (Defu, American fine stem spread alkaline grass and so on) were penetrated with 80 MeV/u 20Ne10+ ions supplied by HIRFL, NLHIAL. Average LET in plateau of Bragg curve of the ions was ...Herbage seeds of legume and grass (Defu, American fine stem spread alkaline grass and so on) were penetrated with 80 MeV/u 20Ne10+ ions supplied by HIRFL, NLHIAL. Average LET in plateau of Bragg curve of the ions was 120 keV/μm. Irradiation dose was 40, 60, 80, 100, 150 Gy for leguminous herbage and 15, 20, 30, 40, 50 Gy for grass herbage. Dose rate of irradiation was 3 Gy/min. Irradiation was carried out in atmosphere. Some of irradiated seeds were sown in the fields and others were used to do experiments for cytology.展开更多
基金Supported by the National Basic Research Program of China(No.2007CB808000)the National Natural Science Foundation of China(Nos.81072337,21074066)
文摘The synthesis of a new type of X-ray-sensitive compound ＂di-（1-hydroxylundecyl） diselenide＂ and its application in the preparation of a new type of liposome with X-ray sensitivity was reported.This new liposome was synthesized to encapsulate doxorubicin hydrochloride（Dox）,with its physical and chemical properties,stability,and radiation sensitivity determined.Based on the pH-gradient method,liposomal Dox was prepared via ultrasonic emulsification and then purified on a Sephadex G50 mini-column.UV spectrophotometry and liquid chromatography were used to detect the encapsulation efficiency and radiation sensitivity of the Dox-loaded liposome.The results show that through changes in release rate,this liposome shows a relative radiosensitivity.In terms of radiation sensitivity,the drug leak rate of the X-ray-sensitive Dox-loaded liposome increased gradually and peaked at 65.4% under the X-ray radiation of a dose of 10 Gy or more than 10 Gy,which is significantly different from that of ordinary liposomes.Meanwhile,X-ray-sensitive Dox-loaded liposome has a good dispersion stability,with an average particle size of approximate 120 nm.The efficiency of this liposome encapsulating Dox was 75.84%,slightly lower than that of ordinary liposomes.The X-ray-sensitive Dox-loaded liposome exhibited suspension stability within 30 d of storage at 4 °C,without visible precipitation.Di-（1-hydroxylundecyl） diselenide is safe and noncytotoxic and compared with those of synthetic phospholipids its synthesis is low cost and does not require complex conditions.
文摘Herbage seeds of legume and grass (Defu, American fine stem spread alkaline grass and so on) were penetrated with 80 MeV/u 20Ne10+ ions supplied by HIRFL, NLHIAL. Average LET in plateau of Bragg curve of the ions was 120 keV/μm. Irradiation dose was 40, 60, 80, 100, 150 Gy for leguminous herbage and 15, 20, 30, 40, 50 Gy for grass herbage. Dose rate of irradiation was 3 Gy/min. Irradiation was carried out in atmosphere. Some of irradiated seeds were sown in the fields and others were used to do experiments for cytology.
