盐胁迫危害植物的各项生理指标,盐浓度过大会影响大豆的生长发育,导致大豆的产量下降。组蛋白的表观修饰,包括甲基化、乙酰化,是表观遗传研究学的一部分,对于基因的表达调控具有重要的意义。植物在生长发育过程中会遇到各种胁迫作用,如...盐胁迫危害植物的各项生理指标,盐浓度过大会影响大豆的生长发育,导致大豆的产量下降。组蛋白的表观修饰,包括甲基化、乙酰化,是表观遗传研究学的一部分,对于基因的表达调控具有重要的意义。植物在生长发育过程中会遇到各种胁迫作用,如非生物胁迫等,而组蛋白的表观修饰在这其中往往发挥着重要作用。该研究以黑河43、黑农69两个品种的大豆为研究对象,构建过表达组蛋白乙酰转移酶基因(GsHAM1)的毛状根,在发根农杆菌介导的遗传转化体系转化组蛋白乙酰转移酶基因30 d后使用50、100、200 mM NaCl处理,观察其表型变化并进行生理实验,进一步了解大豆在盐胁迫下的生理变化,以探索组蛋白乙酰化在盐胁迫中的作用。结果表明,黑河43的耐盐性优于黑农69且过表达GsHAM1基因后可以增强植物的耐盐性能。展开更多
Salinity is one of the major abiotic stresses limiting chickpea(Cicer arietinum L.)productivity,particularly in arid and semi-arid regions where soil salinization is intensifying.Developing cost-effective and practica...Salinity is one of the major abiotic stresses limiting chickpea(Cicer arietinum L.)productivity,particularly in arid and semi-arid regions where soil salinization is intensifying.Developing cost-effective and practical strategies to enhance seedling establishment and early vigor under saline conditions is therefore essential.In this study,we compared two seed-priming agents-1 mM proline and 25 mM NaCl-under identical hydroponic conditions to elucidate tissue-specific responses to 25 mM NaCl stress.Proline priming significantly improved shoot length(by~23%),total chlorophyll content(by~19%),and ascorbate peroxidase(ASPOX)activity.In contrast,NaCl priming enhanced root biomass retention(by~38%)and peroxidase(POD)activity under salinity stress.Both priming treatments induced higher proline accumulation and antioxidant capacity,though with tissue-specific effects:proline favored aboveground resilience,while NaCl strengthened root ionic and oxidative balance.These findings highlight the complementary nature of proline and NaCl priming and support the concept of stress“memory,”whereby plants acquire enhanced readiness to cope with salinity.Integrating such priming strategies into chickpea cultivation could contribute to improved yield stability and sustainability in saline agroecosystems.展开更多
文摘盐胁迫危害植物的各项生理指标,盐浓度过大会影响大豆的生长发育,导致大豆的产量下降。组蛋白的表观修饰,包括甲基化、乙酰化,是表观遗传研究学的一部分,对于基因的表达调控具有重要的意义。植物在生长发育过程中会遇到各种胁迫作用,如非生物胁迫等,而组蛋白的表观修饰在这其中往往发挥着重要作用。该研究以黑河43、黑农69两个品种的大豆为研究对象,构建过表达组蛋白乙酰转移酶基因(GsHAM1)的毛状根,在发根农杆菌介导的遗传转化体系转化组蛋白乙酰转移酶基因30 d后使用50、100、200 mM NaCl处理,观察其表型变化并进行生理实验,进一步了解大豆在盐胁迫下的生理变化,以探索组蛋白乙酰化在盐胁迫中的作用。结果表明,黑河43的耐盐性优于黑农69且过表达GsHAM1基因后可以增强植物的耐盐性能。
文摘Salinity is one of the major abiotic stresses limiting chickpea(Cicer arietinum L.)productivity,particularly in arid and semi-arid regions where soil salinization is intensifying.Developing cost-effective and practical strategies to enhance seedling establishment and early vigor under saline conditions is therefore essential.In this study,we compared two seed-priming agents-1 mM proline and 25 mM NaCl-under identical hydroponic conditions to elucidate tissue-specific responses to 25 mM NaCl stress.Proline priming significantly improved shoot length(by~23%),total chlorophyll content(by~19%),and ascorbate peroxidase(ASPOX)activity.In contrast,NaCl priming enhanced root biomass retention(by~38%)and peroxidase(POD)activity under salinity stress.Both priming treatments induced higher proline accumulation and antioxidant capacity,though with tissue-specific effects:proline favored aboveground resilience,while NaCl strengthened root ionic and oxidative balance.These findings highlight the complementary nature of proline and NaCl priming and support the concept of stress“memory,”whereby plants acquire enhanced readiness to cope with salinity.Integrating such priming strategies into chickpea cultivation could contribute to improved yield stability and sustainability in saline agroecosystems.
