To obtain a new lysine rich protein gene, tomato tsb cDNA was synthesized from nearly matural anther mRNA by RT PCR. Sequence analysis indicated that the isolated fragment was composed of 748 bp, encoding a glutamic a...To obtain a new lysine rich protein gene, tomato tsb cDNA was synthesized from nearly matural anther mRNA by RT PCR. Sequence analysis indicated that the isolated fragment was composed of 748 bp, encoding a glutamic acid rich (22 55%) and lysine rich (14 89%) protein which was hydrophilic throughout and contained seven imperfect repeated motifs of sequence V V E K K N/E E. It had 70 22% homology to the sequence of SB 401 cDNA reported by Liu et al. The deduced amino acid sequence shares 69 96% identity with the reported gene. Southern blot analysis indicated that tsb was one copy in the tomato genome tsb expressed only in pollen.展开更多
文摘To obtain a new lysine rich protein gene, tomato tsb cDNA was synthesized from nearly matural anther mRNA by RT PCR. Sequence analysis indicated that the isolated fragment was composed of 748 bp, encoding a glutamic acid rich (22 55%) and lysine rich (14 89%) protein which was hydrophilic throughout and contained seven imperfect repeated motifs of sequence V V E K K N/E E. It had 70 22% homology to the sequence of SB 401 cDNA reported by Liu et al. The deduced amino acid sequence shares 69 96% identity with the reported gene. Southern blot analysis indicated that tsb was one copy in the tomato genome tsb expressed only in pollen.
