Hyphantria cuea nucleopolyhedrovirus(HcNPV) and several other nucleopolyhedrosis was extracted by differential centrifugation,and purified with sucrose gradient centrifugation method.Polyhedrin was gotten and analysed...Hyphantria cuea nucleopolyhedrovirus(HcNPV) and several other nucleopolyhedrosis was extracted by differential centrifugation,and purified with sucrose gradient centrifugation method.Polyhedrin was gotten and analysed by SDS-PAGE.It indicated that most of those proteins had 32 KD protein band,and with 64 KD protein,and might be the main protein dimer with the structure and found Hyphantria cunea nuclear polyhedrosis virus protein in 18 KD department had a specific band.展开更多
文摘在温度为20～22℃的实验室条件下,用AfMNPV和PrGV的混合病毒悬液感染3龄期菜粉蝶幼虫,可有效降低2种病毒的用量,并显示出良好的增效作用,PrGV和AfMNPV毒力倍数分别为单剂的600倍和400倍以上.不同浓度混合病毒悬液的LT50分别比AfMNPV和PrGV单剂的LT50缩短1.10～2.06 d和0.40～1.93 d.用PrGV病毒悬液感染4龄期菜粉蝶幼虫,其LT50比3龄期增加3.43～6.06 d.
文摘Hyphantria cuea nucleopolyhedrovirus(HcNPV) and several other nucleopolyhedrosis was extracted by differential centrifugation,and purified with sucrose gradient centrifugation method.Polyhedrin was gotten and analysed by SDS-PAGE.It indicated that most of those proteins had 32 KD protein band,and with 64 KD protein,and might be the main protein dimer with the structure and found Hyphantria cunea nuclear polyhedrosis virus protein in 18 KD department had a specific band.
