Cucumber mosaic virus(CMV)threatens lily production by reducing floral quality and enabling carry-over via infected planting stock.To explore tissue-specific host responses,we analyzed a legacy,single-replicate RNA-se...Cucumber mosaic virus(CMV)threatens lily production by reducing floral quality and enabling carry-over via infected planting stock.To explore tissue-specific host responses,we analyzed a legacy,single-replicate RNA-seq dataset from two cultivars,‘Cancun’and‘Connecticut King’(CK),profiling leaf(source)and bulb(sink)tissues at 0 and 28 days post-inoculation(dpi),alongside leaf DAS-ELISA.Principal component analysis indicated that tissue identity dominated the transcriptome(PC1=47.7%),with CMV treatment driving within-tissue shifts over time.Exploratory Gene Ontology/KEGG summaries and a focused marker panel revealed a consistent split:in leaves,genes linked to jasmonate/WRKY-associated defense(e.g.,WRKY40/41/51/53;AOS/OPR1/2;CYP74A/DDE2)tended to show higher expression at 28 dpi,whereas cell-wall/transport-related terms were reduced;in bulbs,transcripts associated with photosynthetic/organellar maintenance(LHCB/CAB,HCF107)andβ-amylase-linked carbohydrate turnover were more prominent,with comparatively limited elevation of canonical defense modules.Leaf ELISA trajectories were compatible with this framework:CK showed a transient peak at 14 dpi followed by a decline at 24 dpi,whereas‘Cancun’increased progressively.Taken together,the concordance among ordination,enrichment patterns,marker behavior,and leaf titers in this non-replicated dataset is consistent with a working model in which stronger or earlier leaf responses may contribute to partial containment and reduced systemic accumulation.We propose a compact leaf marker set(WRKY40/41/51/53;AOS/OPR1/2;CYP74A/DDE2)and bulb candidates(β-amylase;LHCB/CAB/HCF107)as hypothesis-generating indicators of containment and sink maintenance.These tissue-resolved patterns provide a descriptive framework and a starting point for future validation by qPCR and replicated RNA-seq across additional cultivars,with the long-term goal of informing selection and stock hygiene in lily production.展开更多
The strawberry crimp nematode(Aphelenchoides fragariae) is a serious pathogen of ornamental crops and a significant quarantine concern in approximately 50 countries and regions,including China.A nematode population be...The strawberry crimp nematode(Aphelenchoides fragariae) is a serious pathogen of ornamental crops and a significant quarantine concern in approximately 50 countries and regions,including China.A nematode population belonging to the genus Aphelenchoides was isolated from symptomatic leaves of fuchsia plants(Fuchsia×hybrida Hort.ex Sieb.& Voss.) in Chengdu,Sichuan Province,China.Morphological and morphometric characteristics were determined using light microscopy and scanning electron microscopy.Detailed examination revealed diagnostic features consistent with A.fragariae.Three ribosomal DNA(rDNA) regions,i.e.,partial small subunit(SSU) rRNA,D2-D3 expansion segments of the large subunit(LSU) rRNA,and the internal transcribed spacer(ITS),were amplified and sequenced.Bayesian phylogenetic analyses based on these sequences placed the isolate in a well-supported monophyletic clade with reference A.fragariae specimens,clearly separated from other Aphelenchoides species.Furthermore,host-suitability assays demonstrated that this nematode population not only infects and reproduces on Fuchsia×hybrida,but also on Fragaria ananassa and Pteris vittata,two known hosts of A.fragariae.Collectively,morphological,molecular,and host-range evidence confirm the identification of this nematode as A.fragariae.To our knowledge,this represents the first molecular and morphological confirmation of A.fragariae in China,and the first report of Fuchsia×hybrida as a natural host for this species.展开更多
基金the support of“Cooperative Research Program for Agriculture Science and Technology Development(Project No.PJ00926803)”Rural Development Administration,Republic of Korea.
文摘Cucumber mosaic virus(CMV)threatens lily production by reducing floral quality and enabling carry-over via infected planting stock.To explore tissue-specific host responses,we analyzed a legacy,single-replicate RNA-seq dataset from two cultivars,‘Cancun’and‘Connecticut King’(CK),profiling leaf(source)and bulb(sink)tissues at 0 and 28 days post-inoculation(dpi),alongside leaf DAS-ELISA.Principal component analysis indicated that tissue identity dominated the transcriptome(PC1=47.7%),with CMV treatment driving within-tissue shifts over time.Exploratory Gene Ontology/KEGG summaries and a focused marker panel revealed a consistent split:in leaves,genes linked to jasmonate/WRKY-associated defense(e.g.,WRKY40/41/51/53;AOS/OPR1/2;CYP74A/DDE2)tended to show higher expression at 28 dpi,whereas cell-wall/transport-related terms were reduced;in bulbs,transcripts associated with photosynthetic/organellar maintenance(LHCB/CAB,HCF107)andβ-amylase-linked carbohydrate turnover were more prominent,with comparatively limited elevation of canonical defense modules.Leaf ELISA trajectories were compatible with this framework:CK showed a transient peak at 14 dpi followed by a decline at 24 dpi,whereas‘Cancun’increased progressively.Taken together,the concordance among ordination,enrichment patterns,marker behavior,and leaf titers in this non-replicated dataset is consistent with a working model in which stronger or earlier leaf responses may contribute to partial containment and reduced systemic accumulation.We propose a compact leaf marker set(WRKY40/41/51/53;AOS/OPR1/2;CYP74A/DDE2)and bulb candidates(β-amylase;LHCB/CAB/HCF107)as hypothesis-generating indicators of containment and sink maintenance.These tissue-resolved patterns provide a descriptive framework and a starting point for future validation by qPCR and replicated RNA-seq across additional cultivars,with the long-term goal of informing selection and stock hygiene in lily production.
基金financially supported by the Shaanxi Innovation Team Project,China (2024RS-CXTD-73)the National Natural Science Foundation of China (31772136)。
文摘The strawberry crimp nematode(Aphelenchoides fragariae) is a serious pathogen of ornamental crops and a significant quarantine concern in approximately 50 countries and regions,including China.A nematode population belonging to the genus Aphelenchoides was isolated from symptomatic leaves of fuchsia plants(Fuchsia×hybrida Hort.ex Sieb.& Voss.) in Chengdu,Sichuan Province,China.Morphological and morphometric characteristics were determined using light microscopy and scanning electron microscopy.Detailed examination revealed diagnostic features consistent with A.fragariae.Three ribosomal DNA(rDNA) regions,i.e.,partial small subunit(SSU) rRNA,D2-D3 expansion segments of the large subunit(LSU) rRNA,and the internal transcribed spacer(ITS),were amplified and sequenced.Bayesian phylogenetic analyses based on these sequences placed the isolate in a well-supported monophyletic clade with reference A.fragariae specimens,clearly separated from other Aphelenchoides species.Furthermore,host-suitability assays demonstrated that this nematode population not only infects and reproduces on Fuchsia×hybrida,but also on Fragaria ananassa and Pteris vittata,two known hosts of A.fragariae.Collectively,morphological,molecular,and host-range evidence confirm the identification of this nematode as A.fragariae.To our knowledge,this represents the first molecular and morphological confirmation of A.fragariae in China,and the first report of Fuchsia×hybrida as a natural host for this species.
