Ceratocystis fimbriata is a hazardous fungal pathogen that causes black rot disease in sweet potato roots,leading to devastating losses during sweet potato growth and storage.All methods for managing C.fimbriata on sw...Ceratocystis fimbriata is a hazardous fungal pathogen that causes black rot disease in sweet potato roots,leading to devastating losses during sweet potato growth and storage.All methods for managing C.fimbriata on sweet potato based on synthetic fungicides,biocontrol agents,coatings,and natural compounds are reviewed for the first time.The advantages and disadvantages of the different methods are discussed,and their efficacies are compared.Bacillus strains and the synthetic fungicides carbendazim,flutriafol,liguoling,Stadium?,thiabendazole,and thiophanate methyl show the strongest inhibitory effects.Bacillus strains possess an advantage over synthetic fungicides due to the detrimental impact of the latter on human health.The application of filamentous fungi and yeasts as biocontrol agents for C.fimbriata management has not yet been investigated.Coatings exhibit limited protection,and no attempts have been made to combine coatings with nanoparticles or essential oils.Although the natural compounds perillaldehyde and nerol are inexpensive and non-toxic,they are of low efficacy.All management strategies have been used on postharvest sweet potato roots and leaves.However,there is no approach to controlling C.fimbriata in roots during plant growth.Even though several methods for controlling C.fimbriata on sweet potato have been reported in recent years,numerous knowledge gaps impede the development of effective and safe approaches.展开更多
Vascular wilt caused by Fusarium oxysporum f.sp.batatas(Fob)is a devastating disease threatening global sweet potato production.To elucidate Fob’s pathogenicitymechanisms and informeffective control strategies,we gen...Vascular wilt caused by Fusarium oxysporum f.sp.batatas(Fob)is a devastating disease threatening global sweet potato production.To elucidate Fob’s pathogenicitymechanisms and informeffective control strategies,we generated a green fluorescent protein(GFP)-tagged Fob strain to track infection dynamics in sweet potato susceptible cultivar Xinzhonghua and resistant cultivar Xiangshu75-55,respectively.Through cytological observation,we found in the susceptible Xinzhonghua,Fob predominantly colonized stem villi,injured root growth points,and directly invaded vascular bundles through stemwounds.Spore germination peaked at 2-3 h post-inoculation(hpi),followed by cyclical mycelial expansion and sporulation within vascular tissues with sustaining infection.In contrast,the resistant Xiangshu75-55 exhibited strong suppression of Fob:spores rarely germinated in vascular bundles or on trichomes by 3 hpi,and mature hyphae were absent in stems at 24 hpi.Quantitative reverse transcription PCR(qRT-PCR)confirmed significantly higher Fob biomass in Xinzhonghua than in Xiangshu75-55 by 16 hpi.Additionally,transcriptional profiling revealed distinct pathogen-host interactions during the compatible and incompatible reactions.In Xinzhonghua,Fob virulence genes FobPGX1,FobICL1,FobCTF2,FobFUB5 and FobFUB6 were upregulated within 16 hpi.Conversely,host defense genes IbMAPKK9,IbWRKY61,IbWRKY75,IbSWEET10,IbBBX24 and IbPIF4 were activated in Xiangshu75-55 during the same period.This study provides spatiotemporal cytological and molecular insights into Fob pathogenicity and host resistance,offering a foundation for early disease detection and improved Fusarium wilt management in sweet potato.展开更多
Citronellol is a kind of terpene produced by plants in response to external stress;thus can be used as a gas biomarker to detect black spot Ceratocystis fimbriata infection in sweetpotato.However,many contemporary ana...Citronellol is a kind of terpene produced by plants in response to external stress;thus can be used as a gas biomarker to detect black spot Ceratocystis fimbriata infection in sweetpotato.However,many contemporary analytical methods,exemplified by gas chromatography-mass spectrometry,are technically demanding,time-consuming,and require complex sample preparation procedures.In this study,a quartz crystal microbalance(QCM)-based gas sensor fabricated via a surface molecular imprinting technique was modified with a Co/Zn-ZIF@MIP composite,in which cobalt-zinc bimetallic ZIF(Co/Zn-ZIF)served as the support material.A linear relationship was observed between the frequency shift and citronellol concentrations ranging from 0.88 to 22 mg/L,with a sensitivity of−6.08 Hz/(mg·L)and a limit of detection(LOD)of 1.35 mg/L.This result indicated that this sensor has excellent selectivity for citronellol and demonstrates high repeatability,as evidenced by R^(2)value of 0.97.In evaluations with real samples,the sensor reliably identified citronellol among the complex volatile organic compounds(VOCs)emitted from black spot-infected sweetpotato,indicating a high level of selectivity.Our research achieved the rapid characterization of sweetpotato black spot disease within 4 min and provided new insights into the development of QCM-based gas sensors for the rapid assessment of agricultural product quality and safety.展开更多
基金supported by the National Natural Science Foundation of China(Nos.32172441,32302433,and 32001599)。
文摘Ceratocystis fimbriata is a hazardous fungal pathogen that causes black rot disease in sweet potato roots,leading to devastating losses during sweet potato growth and storage.All methods for managing C.fimbriata on sweet potato based on synthetic fungicides,biocontrol agents,coatings,and natural compounds are reviewed for the first time.The advantages and disadvantages of the different methods are discussed,and their efficacies are compared.Bacillus strains and the synthetic fungicides carbendazim,flutriafol,liguoling,Stadium?,thiabendazole,and thiophanate methyl show the strongest inhibitory effects.Bacillus strains possess an advantage over synthetic fungicides due to the detrimental impact of the latter on human health.The application of filamentous fungi and yeasts as biocontrol agents for C.fimbriata management has not yet been investigated.Coatings exhibit limited protection,and no attempts have been made to combine coatings with nanoparticles or essential oils.Although the natural compounds perillaldehyde and nerol are inexpensive and non-toxic,they are of low efficacy.All management strategies have been used on postharvest sweet potato roots and leaves.However,there is no approach to controlling C.fimbriata in roots during plant growth.Even though several methods for controlling C.fimbriata on sweet potato have been reported in recent years,numerous knowledge gaps impede the development of effective and safe approaches.
基金supported by the following grants,Earmarked fund for CARS-10-Sweet potato,High-quality development of agriculture“5511”collaborative innovation project(XTCXGC2021005)Natural Science Foundation of Fujian province(2021J01495)+1 种基金Basic Scientific Research Special Project for Fujian Provincial Public Research Institutes(2021R1031008)Science and Technology Innovation Team of Fujian Academy of Agricultural Sciences(CXTD2021012-1).
文摘Vascular wilt caused by Fusarium oxysporum f.sp.batatas(Fob)is a devastating disease threatening global sweet potato production.To elucidate Fob’s pathogenicitymechanisms and informeffective control strategies,we generated a green fluorescent protein(GFP)-tagged Fob strain to track infection dynamics in sweet potato susceptible cultivar Xinzhonghua and resistant cultivar Xiangshu75-55,respectively.Through cytological observation,we found in the susceptible Xinzhonghua,Fob predominantly colonized stem villi,injured root growth points,and directly invaded vascular bundles through stemwounds.Spore germination peaked at 2-3 h post-inoculation(hpi),followed by cyclical mycelial expansion and sporulation within vascular tissues with sustaining infection.In contrast,the resistant Xiangshu75-55 exhibited strong suppression of Fob:spores rarely germinated in vascular bundles or on trichomes by 3 hpi,and mature hyphae were absent in stems at 24 hpi.Quantitative reverse transcription PCR(qRT-PCR)confirmed significantly higher Fob biomass in Xinzhonghua than in Xiangshu75-55 by 16 hpi.Additionally,transcriptional profiling revealed distinct pathogen-host interactions during the compatible and incompatible reactions.In Xinzhonghua,Fob virulence genes FobPGX1,FobICL1,FobCTF2,FobFUB5 and FobFUB6 were upregulated within 16 hpi.Conversely,host defense genes IbMAPKK9,IbWRKY61,IbWRKY75,IbSWEET10,IbBBX24 and IbPIF4 were activated in Xiangshu75-55 during the same period.This study provides spatiotemporal cytological and molecular insights into Fob pathogenicity and host resistance,offering a foundation for early disease detection and improved Fusarium wilt management in sweet potato.
基金support of the Earmarked Fund for CARS-10-Sweetpotato,China,the National Foundation of Nature and Science of China(Nos.32102083 and M2242001)the Natural Science Foundation of Shandong Province of China(Nos.ZR2021QC204 and ZR2022MC196).
文摘Citronellol is a kind of terpene produced by plants in response to external stress;thus can be used as a gas biomarker to detect black spot Ceratocystis fimbriata infection in sweetpotato.However,many contemporary analytical methods,exemplified by gas chromatography-mass spectrometry,are technically demanding,time-consuming,and require complex sample preparation procedures.In this study,a quartz crystal microbalance(QCM)-based gas sensor fabricated via a surface molecular imprinting technique was modified with a Co/Zn-ZIF@MIP composite,in which cobalt-zinc bimetallic ZIF(Co/Zn-ZIF)served as the support material.A linear relationship was observed between the frequency shift and citronellol concentrations ranging from 0.88 to 22 mg/L,with a sensitivity of−6.08 Hz/(mg·L)and a limit of detection(LOD)of 1.35 mg/L.This result indicated that this sensor has excellent selectivity for citronellol and demonstrates high repeatability,as evidenced by R^(2)value of 0.97.In evaluations with real samples,the sensor reliably identified citronellol among the complex volatile organic compounds(VOCs)emitted from black spot-infected sweetpotato,indicating a high level of selectivity.Our research achieved the rapid characterization of sweetpotato black spot disease within 4 min and provided new insights into the development of QCM-based gas sensors for the rapid assessment of agricultural product quality and safety.
