Phytophthora blight is a devastating disease of pigeon pea(Cajanus cajan)that severely impacts plant growth and productivity.This study investigates the morphological,anatomical,and biochemical responses of a suscepti...Phytophthora blight is a devastating disease of pigeon pea(Cajanus cajan)that severely impacts plant growth and productivity.This study investigates the morphological,anatomical,and biochemical responses of a susceptible variety(ICPL 11260)and a resistant variety(IPAC-02)following infection by Phytophthora.Morphological analyses showed that infection caused a drastic reduction in root length,shoot length,leaf number,fresh weight,and dry weight in the susceptible ICPL 11260 variety,with reductions ranging from 0.5-to 2-fold compared to non-infected controls.Anatomical observations revealed pronounced cellular damage and mycelial invasion in infected ICPL 11260 plants by 30 days after infection,whereas infected IPAC-02 plants exhibited no fungal colonization.Biochemical analyses further demonstrated that the resistant IPAC-02 variety accumulated higher levels of total soluble sugars,proteins,phenols,and flavonoids,along with increased activities of defense-related enzymes(chitinase andβ-1,3-glucanase),compared with the susceptible ICPL 11260.Under P.cajani stress,IPAC-02 maintained significantly elevated osmolyte concentrations(total sugars 153.7 mg g^(−1)FW;proteins 25.4 mg g^(−1)FW),secondary metabolites(phenols 51.7 mg g^(−1)FW;flavonoids 33.1 mg g^(−1)FW),and PR-enzyme activities(chitinase 11.4 U mg^(−1)protein;β-1,3-glucanase 9.1 U mg^(−1)protein).These responses support a lignification-mediated defense mechanism in IPAC-02 and highlight its potential value for breeding Phytophthora-resistant pigeon pea cultivars.展开更多
为了明确紫外线(UV-B)辐射对红色型和绿色型豌豆蚜生物学特性的影响,本研究将2种不同色型豌豆蚜的初生若蚜经过40 W UV-B波段紫外线每日照射30 min,在3种温度梯度(16℃、20℃、24℃)和2种苜蓿品种"甘农3号"和"甘农5号&qu...为了明确紫外线(UV-B)辐射对红色型和绿色型豌豆蚜生物学特性的影响,本研究将2种不同色型豌豆蚜的初生若蚜经过40 W UV-B波段紫外线每日照射30 min,在3种温度梯度(16℃、20℃、24℃)和2种苜蓿品种"甘农3号"和"甘农5号"条件下,在人工气候箱中单头饲养,测定各个处理豌豆蚜的发育历期、体质量差和相对日均体质量增长率等生物学参数。结果显示:在"甘农3号"上,16℃时,在UV-B辐射对2种色型豌豆蚜的发育历期、体质量差和日均体质量增长率均无显著影响(P>0.05);在20℃时,UV-B辐射下红色型豌豆蚜的发育历期显著延长(P<0.05),相对日均体质量增长率也显著减少(P<0.05),体质量差无显著变化(P>0.05);而在24℃时,UV-B辐射下绿色型豌豆蚜的发育历期显著延长(P<0.05),体质量差和相对日均体质量增长率也显著减少(P<0.05)。在"甘农5号"上,在16℃时,UV-B辐射下红色型豌豆蚜的发育历期显著延长(P<0.05),体质量差和相对日均体质量增长率也显著减少(P<0.05);在20℃和24℃时,UV-B辐射绿色型豌豆蚜的发育历期显著延长(P<0.05),体质量差和相对日均体质量增长率也显著减少(P<0.05)。由此可知,UV-B辐射对豌豆蚜的影响与温度和苜蓿品种均密切相关。展开更多
对天津地区保护地菜豆菌核病病原菌进行分离纯化,在实验室条件下对病原菌的形态特征、致病性和生物学特性进行了研究,结果表明病原菌为核盘菌[Sclerotinia sclerotiorum(Lib) de Bary.]。此菌在多种培养基上均可生长,最适生长培养基为PD...对天津地区保护地菜豆菌核病病原菌进行分离纯化,在实验室条件下对病原菌的形态特征、致病性和生物学特性进行了研究,结果表明病原菌为核盘菌[Sclerotinia sclerotiorum(Lib) de Bary.]。此菌在多种培养基上均可生长,最适生长培养基为PDA。菌落生长温度范围为5～30℃,最适菌落生长温度为18～23℃。菌丝生长的适宜湿度为90%～100%;在所测试的碳源中,病原菌对乳糖利用最好;在供试的氮源中,对硫酸铵利用最好;菌丝在pH3～10均可生长,生长的最佳pH值为6.5。展开更多
基金supported and funded by the Deanship of Scientific Research,Vice Presidency for Graduate Studies and Scientific Research,King Faisal University,Saudi Arabia,grant number(KFU252909).
文摘Phytophthora blight is a devastating disease of pigeon pea(Cajanus cajan)that severely impacts plant growth and productivity.This study investigates the morphological,anatomical,and biochemical responses of a susceptible variety(ICPL 11260)and a resistant variety(IPAC-02)following infection by Phytophthora.Morphological analyses showed that infection caused a drastic reduction in root length,shoot length,leaf number,fresh weight,and dry weight in the susceptible ICPL 11260 variety,with reductions ranging from 0.5-to 2-fold compared to non-infected controls.Anatomical observations revealed pronounced cellular damage and mycelial invasion in infected ICPL 11260 plants by 30 days after infection,whereas infected IPAC-02 plants exhibited no fungal colonization.Biochemical analyses further demonstrated that the resistant IPAC-02 variety accumulated higher levels of total soluble sugars,proteins,phenols,and flavonoids,along with increased activities of defense-related enzymes(chitinase andβ-1,3-glucanase),compared with the susceptible ICPL 11260.Under P.cajani stress,IPAC-02 maintained significantly elevated osmolyte concentrations(total sugars 153.7 mg g^(−1)FW;proteins 25.4 mg g^(−1)FW),secondary metabolites(phenols 51.7 mg g^(−1)FW;flavonoids 33.1 mg g^(−1)FW),and PR-enzyme activities(chitinase 11.4 U mg^(−1)protein;β-1,3-glucanase 9.1 U mg^(−1)protein).These responses support a lignification-mediated defense mechanism in IPAC-02 and highlight its potential value for breeding Phytophthora-resistant pigeon pea cultivars.
文摘为了明确紫外线(UV-B)辐射对红色型和绿色型豌豆蚜生物学特性的影响,本研究将2种不同色型豌豆蚜的初生若蚜经过40 W UV-B波段紫外线每日照射30 min,在3种温度梯度(16℃、20℃、24℃)和2种苜蓿品种"甘农3号"和"甘农5号"条件下,在人工气候箱中单头饲养,测定各个处理豌豆蚜的发育历期、体质量差和相对日均体质量增长率等生物学参数。结果显示:在"甘农3号"上,16℃时,在UV-B辐射对2种色型豌豆蚜的发育历期、体质量差和日均体质量增长率均无显著影响(P>0.05);在20℃时,UV-B辐射下红色型豌豆蚜的发育历期显著延长(P<0.05),相对日均体质量增长率也显著减少(P<0.05),体质量差无显著变化(P>0.05);而在24℃时,UV-B辐射下绿色型豌豆蚜的发育历期显著延长(P<0.05),体质量差和相对日均体质量增长率也显著减少(P<0.05)。在"甘农5号"上,在16℃时,UV-B辐射下红色型豌豆蚜的发育历期显著延长(P<0.05),体质量差和相对日均体质量增长率也显著减少(P<0.05);在20℃和24℃时,UV-B辐射绿色型豌豆蚜的发育历期显著延长(P<0.05),体质量差和相对日均体质量增长率也显著减少(P<0.05)。由此可知,UV-B辐射对豌豆蚜的影响与温度和苜蓿品种均密切相关。
文摘对天津地区保护地菜豆菌核病病原菌进行分离纯化,在实验室条件下对病原菌的形态特征、致病性和生物学特性进行了研究,结果表明病原菌为核盘菌[Sclerotinia sclerotiorum(Lib) de Bary.]。此菌在多种培养基上均可生长,最适生长培养基为PDA。菌落生长温度范围为5～30℃,最适菌落生长温度为18～23℃。菌丝生长的适宜湿度为90%～100%;在所测试的碳源中,病原菌对乳糖利用最好;在供试的氮源中,对硫酸铵利用最好;菌丝在pH3～10均可生长,生长的最佳pH值为6.5。
