[Objectives]This study was conducted to find out regulatory genes related to purple in spears of asparagus(Asparagus officinalis L.).[Methods]The stable asparagus inbred line JX1513-5(the base of the spear is purple)a...[Objectives]This study was conducted to find out regulatory genes related to purple in spears of asparagus(Asparagus officinalis L.).[Methods]The stable asparagus inbred line JX1513-5(the base of the spear is purple)and JLV1718-7(the base of the spear is green)were used as parents to study the genetic law of purple/green traits in their offspring.[Results]The results showed that the purple in the basal part of asparagus spear was controlled by a pair of alleles,and purple was dominant over green.The F 2 segregation population was resequenced by the bulk segregation analysis(BSA)method,and the purple trait in the basal part of asparagus spear was located in the interval of 24.51-25.08 Mb on Chr07 chromosome,which included 47 genes.According to the annotation information,three candidate genes were screened out:LOC109849403,LOC109849430 and LOC109849442.The candidate genes were verified by real-time fluorescence quantitative PCR(qRT-PCR),and finally LOC109849442 was obtained as the candidate gene for controlling the purple/green trait in the basal part of asparagus spear.[Conclusions]This study lays a foundation for the breeding of new asparagus varieties and molecular marker-assisted breeding.展开更多
The test on the preference of Thrips palmi to eight color sticky cards was carried out in an eggplant field. The thrip had the strongest preference to blue sticky card (P<0.01), and its preference order to the othe...The test on the preference of Thrips palmi to eight color sticky cards was carried out in an eggplant field. The thrip had the strongest preference to blue sticky card (P<0.01), and its preference order to the other 7 color cards was as follows: blue, turguoise, yellow, deep blue, green, orange, red and black. Results on the trapping effect of blue sticky card from east, south, west and north directions show that most thrips were trapped from the north,which existed a significant difference with those from the other three directions (P<0.05). Five blue sticky cards were set up at the heights of 73.9,101.7,129.5,157.3 and 185.1 cm above the ground to trap thrips, when the average height of eggplant was approximately 70 cm. More thrips were trapped of 73.9 and 101.7 cm height, which existed significant difference with those at the other three heights. During 5 continuous 3 hour spans from 5 am to 8 pm, the numbers of female, male and total adult thrips trapped were not significantly different.展开更多
基金Supported by S&T Program of Hebei (22326309D)HAAFS Science and Technology Innovation Special Project (2022KJCXZX-JZS-08).
文摘[Objectives]This study was conducted to find out regulatory genes related to purple in spears of asparagus(Asparagus officinalis L.).[Methods]The stable asparagus inbred line JX1513-5(the base of the spear is purple)and JLV1718-7(the base of the spear is green)were used as parents to study the genetic law of purple/green traits in their offspring.[Results]The results showed that the purple in the basal part of asparagus spear was controlled by a pair of alleles,and purple was dominant over green.The F 2 segregation population was resequenced by the bulk segregation analysis(BSA)method,and the purple trait in the basal part of asparagus spear was located in the interval of 24.51-25.08 Mb on Chr07 chromosome,which included 47 genes.According to the annotation information,three candidate genes were screened out:LOC109849403,LOC109849430 and LOC109849442.The candidate genes were verified by real-time fluorescence quantitative PCR(qRT-PCR),and finally LOC109849442 was obtained as the candidate gene for controlling the purple/green trait in the basal part of asparagus spear.[Conclusions]This study lays a foundation for the breeding of new asparagus varieties and molecular marker-assisted breeding.
文摘The test on the preference of Thrips palmi to eight color sticky cards was carried out in an eggplant field. The thrip had the strongest preference to blue sticky card (P<0.01), and its preference order to the other 7 color cards was as follows: blue, turguoise, yellow, deep blue, green, orange, red and black. Results on the trapping effect of blue sticky card from east, south, west and north directions show that most thrips were trapped from the north,which existed a significant difference with those from the other three directions (P<0.05). Five blue sticky cards were set up at the heights of 73.9,101.7,129.5,157.3 and 185.1 cm above the ground to trap thrips, when the average height of eggplant was approximately 70 cm. More thrips were trapped of 73.9 and 101.7 cm height, which existed significant difference with those at the other three heights. During 5 continuous 3 hour spans from 5 am to 8 pm, the numbers of female, male and total adult thrips trapped were not significantly different.
