Spider mites are significant pests in agricultural production.The increasing resistance of spider mites,along with environmental pollution and ecological imbalance caused by their control,is primarily attributed to th...Spider mites are significant pests in agricultural production.The increasing resistance of spider mites,along with environmental pollution and ecological imbalance caused by their control,is primarily attributed to the long-term use of chemical acaricides in agriculture.In contrast,bioactive substances of biological origin offer advantages such as wide availability,environmental friendliness,and low tendency to induce resistance,making them a research hotspot for spider mite control.This review summarizes recent advances in the use of plant-derived active compounds(exemplified by extracts from Veratrum rhizomes),RNA interference(RNAi)technology,and microorganism-derived active substances for controlling spider mites.These bioactive agents exert acaricidal effects by disrupting the nervous system,interfering with metabolic processes,or silencing key genes in mites,demonstrating favorable efficacy and considerable potential for development.However,challenges remain,including poor environmental stability,slow action,high production costs,and insufficient understanding of their effects on non-target organisms.Therefore,future research should focus on the screening and development of novel bioactive substances of biological origin,elucidation of their mechanisms of action,optimization of formulation technologies,and assessment of their ecological safety.These efforts will provide valuable insights for promoting the advancement of bioactive substances and supporting sustainable agricultural development.展开更多
为了探究不同内共生菌感染品系对土耳其斯坦叶螨生殖的调控效果,通过系统发育分析沃尔巴克氏体(Wolbachia)、Cardinium所属群组,用RNA-Seq对分别感染Wolbachia、Cardinium及共同感染Wolbachia、Cardinium的雌成螨进行转录组学分析,并用...为了探究不同内共生菌感染品系对土耳其斯坦叶螨生殖的调控效果,通过系统发育分析沃尔巴克氏体(Wolbachia)、Cardinium所属群组,用RNA-Seq对分别感染Wolbachia、Cardinium及共同感染Wolbachia、Cardinium的雌成螨进行转录组学分析,并用加权基因共表达网络分析(WGCNA)筛选与生殖调控相关的基因。结果表明,Wolbachia属于B群,Cardinium归属于A群。不同内共生菌感染对土耳其斯坦叶螨转录水平产生了显著影响,其中A-WC-F vs A-F的差异基因最多,而A-W-F vs A-F的差异基因最少。基因本体(GO)功能富集结果显示,A-W-F vs A-F、A-C-F vs A-F均富集在蛋白质去泛素化、泛素蛋白转移酶活性等条目,A-WC-F vs A-F富集到蛋白水解、脂质转运体活性等条目。KEGG通路富集结果显示,3个比较组合均富集到FoxO信号通路、Wnt信号通路,A-W-F vs A-F、A-C-F vs A-F还富集到泛素介导的蛋白水解。对生殖调控相关的差异表达基因进行分析,结果显示,大部分基因与泛素化相关,如编码Fizzled、CK1、E1、E2和E3等的基因。进一步通过WGCNA进行分析,鉴定出2个模块与内共生菌感染高度相关,并筛选出调控生殖相关的关键基因,这些基因可能在生殖调控中发挥重要作用。研究结果可为深入探讨内共生菌对宿主生殖调控机制提供参考。展开更多
文摘Spider mites are significant pests in agricultural production.The increasing resistance of spider mites,along with environmental pollution and ecological imbalance caused by their control,is primarily attributed to the long-term use of chemical acaricides in agriculture.In contrast,bioactive substances of biological origin offer advantages such as wide availability,environmental friendliness,and low tendency to induce resistance,making them a research hotspot for spider mite control.This review summarizes recent advances in the use of plant-derived active compounds(exemplified by extracts from Veratrum rhizomes),RNA interference(RNAi)technology,and microorganism-derived active substances for controlling spider mites.These bioactive agents exert acaricidal effects by disrupting the nervous system,interfering with metabolic processes,or silencing key genes in mites,demonstrating favorable efficacy and considerable potential for development.However,challenges remain,including poor environmental stability,slow action,high production costs,and insufficient understanding of their effects on non-target organisms.Therefore,future research should focus on the screening and development of novel bioactive substances of biological origin,elucidation of their mechanisms of action,optimization of formulation technologies,and assessment of their ecological safety.These efforts will provide valuable insights for promoting the advancement of bioactive substances and supporting sustainable agricultural development.
文摘为了探究不同内共生菌感染品系对土耳其斯坦叶螨生殖的调控效果,通过系统发育分析沃尔巴克氏体(Wolbachia)、Cardinium所属群组,用RNA-Seq对分别感染Wolbachia、Cardinium及共同感染Wolbachia、Cardinium的雌成螨进行转录组学分析,并用加权基因共表达网络分析(WGCNA)筛选与生殖调控相关的基因。结果表明,Wolbachia属于B群,Cardinium归属于A群。不同内共生菌感染对土耳其斯坦叶螨转录水平产生了显著影响,其中A-WC-F vs A-F的差异基因最多,而A-W-F vs A-F的差异基因最少。基因本体(GO)功能富集结果显示,A-W-F vs A-F、A-C-F vs A-F均富集在蛋白质去泛素化、泛素蛋白转移酶活性等条目,A-WC-F vs A-F富集到蛋白水解、脂质转运体活性等条目。KEGG通路富集结果显示,3个比较组合均富集到FoxO信号通路、Wnt信号通路,A-W-F vs A-F、A-C-F vs A-F还富集到泛素介导的蛋白水解。对生殖调控相关的差异表达基因进行分析,结果显示,大部分基因与泛素化相关,如编码Fizzled、CK1、E1、E2和E3等的基因。进一步通过WGCNA进行分析,鉴定出2个模块与内共生菌感染高度相关,并筛选出调控生殖相关的关键基因,这些基因可能在生殖调控中发挥重要作用。研究结果可为深入探讨内共生菌对宿主生殖调控机制提供参考。
