目的分析1株产酸克雷伯菌噬菌体(KOX20)的生物学特性,探讨其在防治产酸克雷伯菌感染性疾病方面的应用价值。方法于2024年通过双层琼脂平板法观察产酸克雷伯菌噬菌体(KOX20)噬菌斑形态,电子显微镜观察产酸克雷伯菌体(KOX20)的大小与外观...目的分析1株产酸克雷伯菌噬菌体(KOX20)的生物学特性,探讨其在防治产酸克雷伯菌感染性疾病方面的应用价值。方法于2024年通过双层琼脂平板法观察产酸克雷伯菌噬菌体(KOX20)噬菌斑形态,电子显微镜观察产酸克雷伯菌体(KOX20)的大小与外观结构,测定其效价、最佳感染复数(multiplicity of infection,MOI)、一步生长曲线、宿主谱、温度耐受性、紫外线耐受性以及pH的耐受程度。结果产酸克雷伯菌噬菌体(KOX20)在双层琼脂平板上形成边缘整齐、亮度高、无晕环、直径大约1mm的噬菌斑。产酸克雷伯菌噬菌体(KOX20)电镜下头部直径约为80nm,尾部长度约为250nm,属于长尾噬菌体,效价为1.03×10^(9)PFU/ml,最佳感染复数为1000,潜伏期为30min,裂解期为30min,宿主谱较窄,只能裂解宿主菌和1株肺炎克雷伯菌,在4~50℃环境下裂解活性保持稳定,超过60℃完全失活,裂解活性在pH值为6~11时保持稳定,pH值为1~3或pH值为12~14时完全失活,紫外线照射30min内可以保持活性,随着时间的延长,产酸克雷伯菌噬菌体(KOX20)裂解活性下降。结论产酸克雷伯菌噬菌体(KOX20)是1株宿主谱较窄、潜伏期较短、具有良好温度和pH敏感性且紫外线耐受能力弱的长尾噬菌体,具有应用于个性化临床治疗的潜力。展开更多
new heterocyclic dipeptide with a highly functionalized 1,2-oxazadecaline core,named trichodermamide H(1),and three known analogues,along with three known polyketides,were isolated from the fermentation extract of the...new heterocyclic dipeptide with a highly functionalized 1,2-oxazadecaline core,named trichodermamide H(1),and three known analogues,along with three known polyketides,were isolated from the fermentation extract of the mangrovederived fungus Penicillium janthinellum XLN32122.The structure of 1 was elucidated on the basis of extensive 1D and 2D NMR spectra data analysis,HR-ESI-MS,electronic circular dichroism(ECD)calculations.Trichodermamide B(3)exhibited better inhibitory effect on nitric oxide(NO)production in lipopolysaccharide(LPS)induced RAW 264.7 cells with an IC_(50)value of(13.13±0.005)μmol/L than that of the positive control dexamethasone[IC_(50)=(136.84±1.33)μmol/L].Compound 3 exhibited antibacterial activity against methicillin-resistant Staphylococcus aureus(MRSA)with an IC_(50)value of 12.5μg/mL,while the positive control vancomycin showed an IC_(50)value of 1.563μg/mL.展开更多
背景:人羊膜作为一种天然生物材料,因优异的生物相容性、低免疫原性和丰富的生物活性物质,在组织工程学中展现出广泛应用前景。近年来,针对人羊膜的储存、制备及在组织修复和再生医学中的研究不断深入,推动了多领域的转化与临床应用。目...背景:人羊膜作为一种天然生物材料,因优异的生物相容性、低免疫原性和丰富的生物活性物质,在组织工程学中展现出广泛应用前景。近年来,针对人羊膜的储存、制备及在组织修复和再生医学中的研究不断深入,推动了多领域的转化与临床应用。目的:综述人羊膜的储存、制备、改性及在组织工程和再生医学中的研究进展,为人羊膜的进一步开发与临床应用提供理论依据。方法:通过计算机检索中国知网、中国生物医学文献数据库、万方数据库、PubMed、Web of Science、Scopus以及Elsevier数据库,中文检索词为“人羊膜,人羊膜的储存,人羊膜的改性,人羊膜的应用,组织工程学”。英文检索词为“Human amniotic membrane,modification of human amniotic membrane,Amniotic membrane modification,Tissue engineering,Stem cells,Bioactive factors,Scaffold,Regenerative medicine”。对初步检索到的文章标题和摘要进行阅读,排除不符合要求的文章,随后根据纳入及排除标准对文章全文进行阅读,最终纳入72篇符合要求的文章。结果与结论:人羊膜具有出色的生物相容性且含有多种活性因子,通过对人羊膜储存和改性技术的不断优化,其应用形式得到进一步拓展,如支架材料、补片等应用形式,同时其力学性能、降解速率和细胞黏附能力都得到了有效提升,可为软组织修复和再生提供支持,未来应关注人羊膜与多功能材料的复合应用,深入研究人羊膜作为组织支架、补片及活性因子载体等的作用机制,进一步提升人羊膜在再生医学中的应用价值。展开更多
Hyperactivation of DNA repairing pathway is highly associated with the chemosensitivity and chemoresistance of cancer cells.In this manuscript,guided by cascaded one strain many compounds-global natural products socia...Hyperactivation of DNA repairing pathway is highly associated with the chemosensitivity and chemoresistance of cancer cells.In this manuscript,guided by cascaded one strain many compounds-global natural products social molecular networking(OSMAC-GNPS)strategy,a pair of epimeric environmental-induced metabolites were isolated from Aspergillus sp.EGF 15-0-3.Structurally,sterpiperazines A(1)and B(2)represent the first steroid-based indole alkaloids with unprecedented backbones.Biologically,compound 1 could be identified as a novel tyrosyl-DNA phosphodiesterase 1(Tdp1)inhibitor with a unique mechanism distinct from the reported modulators,and was able to significantly enhance the sensitivity of NCI-H460 cells to the clinic chemotherapeutic drug through inhibiting the DNA repairment and enhanced the DNA damage of cancer cells.展开更多
文摘目的分析1株产酸克雷伯菌噬菌体(KOX20)的生物学特性,探讨其在防治产酸克雷伯菌感染性疾病方面的应用价值。方法于2024年通过双层琼脂平板法观察产酸克雷伯菌噬菌体(KOX20)噬菌斑形态,电子显微镜观察产酸克雷伯菌体(KOX20)的大小与外观结构,测定其效价、最佳感染复数(multiplicity of infection,MOI)、一步生长曲线、宿主谱、温度耐受性、紫外线耐受性以及pH的耐受程度。结果产酸克雷伯菌噬菌体(KOX20)在双层琼脂平板上形成边缘整齐、亮度高、无晕环、直径大约1mm的噬菌斑。产酸克雷伯菌噬菌体(KOX20)电镜下头部直径约为80nm,尾部长度约为250nm,属于长尾噬菌体,效价为1.03×10^(9)PFU/ml,最佳感染复数为1000,潜伏期为30min,裂解期为30min,宿主谱较窄,只能裂解宿主菌和1株肺炎克雷伯菌,在4~50℃环境下裂解活性保持稳定,超过60℃完全失活,裂解活性在pH值为6~11时保持稳定,pH值为1~3或pH值为12~14时完全失活,紫外线照射30min内可以保持活性,随着时间的延长,产酸克雷伯菌噬菌体(KOX20)裂解活性下降。结论产酸克雷伯菌噬菌体(KOX20)是1株宿主谱较窄、潜伏期较短、具有良好温度和pH敏感性且紫外线耐受能力弱的长尾噬菌体,具有应用于个性化临床治疗的潜力。
基金Project supported by the National Natural Science Foundation of China(No.32160108)the Science and Technology Special Fund of Hainan Province(No.ZDYF2024SHFZ116)+2 种基金the Specific Research Fund of the Innovation Center for Academicians of Hainan Province(No.YSPTZX202309)the Scientific Research Project of Hainan Higher Education Institutions(No.Hnky2022ZD-6)the Hainan Normal University National College Student Innovation Training Program(No.202411658006)。
文摘new heterocyclic dipeptide with a highly functionalized 1,2-oxazadecaline core,named trichodermamide H(1),and three known analogues,along with three known polyketides,were isolated from the fermentation extract of the mangrovederived fungus Penicillium janthinellum XLN32122.The structure of 1 was elucidated on the basis of extensive 1D and 2D NMR spectra data analysis,HR-ESI-MS,electronic circular dichroism(ECD)calculations.Trichodermamide B(3)exhibited better inhibitory effect on nitric oxide(NO)production in lipopolysaccharide(LPS)induced RAW 264.7 cells with an IC_(50)value of(13.13±0.005)μmol/L than that of the positive control dexamethasone[IC_(50)=(136.84±1.33)μmol/L].Compound 3 exhibited antibacterial activity against methicillin-resistant Staphylococcus aureus(MRSA)with an IC_(50)value of 12.5μg/mL,while the positive control vancomycin showed an IC_(50)value of 1.563μg/mL.
文摘背景:人羊膜作为一种天然生物材料,因优异的生物相容性、低免疫原性和丰富的生物活性物质,在组织工程学中展现出广泛应用前景。近年来,针对人羊膜的储存、制备及在组织修复和再生医学中的研究不断深入,推动了多领域的转化与临床应用。目的:综述人羊膜的储存、制备、改性及在组织工程和再生医学中的研究进展,为人羊膜的进一步开发与临床应用提供理论依据。方法:通过计算机检索中国知网、中国生物医学文献数据库、万方数据库、PubMed、Web of Science、Scopus以及Elsevier数据库,中文检索词为“人羊膜,人羊膜的储存,人羊膜的改性,人羊膜的应用,组织工程学”。英文检索词为“Human amniotic membrane,modification of human amniotic membrane,Amniotic membrane modification,Tissue engineering,Stem cells,Bioactive factors,Scaffold,Regenerative medicine”。对初步检索到的文章标题和摘要进行阅读,排除不符合要求的文章,随后根据纳入及排除标准对文章全文进行阅读,最终纳入72篇符合要求的文章。结果与结论:人羊膜具有出色的生物相容性且含有多种活性因子,通过对人羊膜储存和改性技术的不断优化,其应用形式得到进一步拓展,如支架材料、补片等应用形式,同时其力学性能、降解速率和细胞黏附能力都得到了有效提升,可为软组织修复和再生提供支持,未来应关注人羊膜与多功能材料的复合应用,深入研究人羊膜作为组织支架、补片及活性因子载体等的作用机制,进一步提升人羊膜在再生医学中的应用价值。
基金supported by the National Natural Science Foundation of China(Nos.82273845,82304331,and 82360695)Guangxi Natural Science Foundation project(No.2023GXNSFBA026305).
文摘Hyperactivation of DNA repairing pathway is highly associated with the chemosensitivity and chemoresistance of cancer cells.In this manuscript,guided by cascaded one strain many compounds-global natural products social molecular networking(OSMAC-GNPS)strategy,a pair of epimeric environmental-induced metabolites were isolated from Aspergillus sp.EGF 15-0-3.Structurally,sterpiperazines A(1)and B(2)represent the first steroid-based indole alkaloids with unprecedented backbones.Biologically,compound 1 could be identified as a novel tyrosyl-DNA phosphodiesterase 1(Tdp1)inhibitor with a unique mechanism distinct from the reported modulators,and was able to significantly enhance the sensitivity of NCI-H460 cells to the clinic chemotherapeutic drug through inhibiting the DNA repairment and enhanced the DNA damage of cancer cells.
