OBJECTIVE:To establish an in vitro model of radiation-induced lung injury using rat lung alveolar macrophages(NR8383).METHODS:Using a medical electronic linear accelerator,cells were irradiated with either 0 Gy or 6 G...OBJECTIVE:To establish an in vitro model of radiation-induced lung injury using rat lung alveolar macrophages(NR8383).METHODS:Using a medical electronic linear accelerator,cells were irradiated with either 0 Gy or 6 Gy X-rays.At 6,12,24,30 and 48 h,the DNA damage index(8-OHd G)and lipid damage index(MDA)were measured in the two groups.We also determined the levels of tumor necrosis factor-alpha(TNF-α),interleukin-6(IL-6)and transforming growth factor-β(TGF-β).RESULTS:The levels of 8-OHd G and MDA in the 6 Gy irradiation group were higher than those in the0 Gy group at 6,12,24,30 and 48 h after irradiation.The levels reached the highest value-6 h after irradiation,and then gradually decreased.The levels of the inflammatory factors TNF-α,TGF-βand IL-6 were higher in the 6 Gy irradiation group than those in the 0 Gy group at 6,12,24,30 and 48 h after irradiation.CONCLUSION:Six Gy X-ray irradiated NR8383 cells can be used to establish an in-vitro model of radiation-induced lung injury.The levels of 8-OHd G,MDA,TNF-α,TGF-βand IL-6 can be used as effective evaluation indicators.展开更多
背景:研究表明,抗阻运动不仅通过机械应力直接刺激骨组织,还可通过诱导肌肉分泌多种肌因子间接调控骨代谢。目的:从“肌骨系统”整体视角探讨抗阻运动干预下肌因子对骨骼的调控机制。方法:检索Web of Science、PubMed、中国知网、万方...背景:研究表明,抗阻运动不仅通过机械应力直接刺激骨组织,还可通过诱导肌肉分泌多种肌因子间接调控骨代谢。目的:从“肌骨系统”整体视角探讨抗阻运动干预下肌因子对骨骼的调控机制。方法:检索Web of Science、PubMed、中国知网、万方和维普数据库从建库至2024年7月的相关文献,以“resistance exercise,myokines,bone metabolism,osteoporosis,sarcopenia”为英文检索词,“抗阻运动,肌因子,骨代谢,骨质疏松症,肌肉减少症”为中文检索词,最终纳入59篇文献进行综述。结果与结论:研究发现肌肉生长抑制素在肌肉生长和发育中起负调控作用,肌肉生长抑制素减少可促进肌肉增长。抗阻运动可能通过降低肌肉生长抑制素水平间接促进骨骼健康,但具体机制尚需进一步探究。鸢尾素可促进骨形成,而抗阻运动可显著提高鸢尾素水平,并可能通过激活Wnt/β-连环蛋白信号通路等促进骨细胞增殖和分化。基质金属蛋白酶2在骨重塑过程中发挥重要作用,它能够降解骨基质成分、维持骨吸收和形成之间的平衡。抗阻运动通过调节基质金属蛋白酶2活性可能有助于维持骨组织的正常结构和功能。胰岛素样生长因子1对骨骼生长和发育具有显著促进作用。抗阻运动能够增加胰岛素样生长因子1的表达和分泌,进而促进骨细胞增殖、分化和矿化,提高骨密度和强度。抗阻运动通过调节肌肉生长抑制素、鸢尾素、基质金属蛋白酶2和胰岛素样生长因子1等肌因子的表达和活性,对骨代谢产生积极影响,有助于预防和治疗骨质疏松症,然而具体机制仍需深入研究,以进一步阐明抗阻运动对肌骨系统的整体调控作用。展开更多
基金Supported by the National Natural Science Foundation of China the Mechanism Underlying Nourishing Yin,Clearing Lung and Activating Blood Circulation to Regulate AMPK Mediated Autophagy in Radiation-induced Lung Injury(No.81673754)。
文摘OBJECTIVE:To establish an in vitro model of radiation-induced lung injury using rat lung alveolar macrophages(NR8383).METHODS:Using a medical electronic linear accelerator,cells were irradiated with either 0 Gy or 6 Gy X-rays.At 6,12,24,30 and 48 h,the DNA damage index(8-OHd G)and lipid damage index(MDA)were measured in the two groups.We also determined the levels of tumor necrosis factor-alpha(TNF-α),interleukin-6(IL-6)and transforming growth factor-β(TGF-β).RESULTS:The levels of 8-OHd G and MDA in the 6 Gy irradiation group were higher than those in the0 Gy group at 6,12,24,30 and 48 h after irradiation.The levels reached the highest value-6 h after irradiation,and then gradually decreased.The levels of the inflammatory factors TNF-α,TGF-βand IL-6 were higher in the 6 Gy irradiation group than those in the 0 Gy group at 6,12,24,30 and 48 h after irradiation.CONCLUSION:Six Gy X-ray irradiated NR8383 cells can be used to establish an in-vitro model of radiation-induced lung injury.The levels of 8-OHd G,MDA,TNF-α,TGF-βand IL-6 can be used as effective evaluation indicators.
文摘背景:研究表明,抗阻运动不仅通过机械应力直接刺激骨组织,还可通过诱导肌肉分泌多种肌因子间接调控骨代谢。目的:从“肌骨系统”整体视角探讨抗阻运动干预下肌因子对骨骼的调控机制。方法:检索Web of Science、PubMed、中国知网、万方和维普数据库从建库至2024年7月的相关文献,以“resistance exercise,myokines,bone metabolism,osteoporosis,sarcopenia”为英文检索词,“抗阻运动,肌因子,骨代谢,骨质疏松症,肌肉减少症”为中文检索词,最终纳入59篇文献进行综述。结果与结论:研究发现肌肉生长抑制素在肌肉生长和发育中起负调控作用,肌肉生长抑制素减少可促进肌肉增长。抗阻运动可能通过降低肌肉生长抑制素水平间接促进骨骼健康,但具体机制尚需进一步探究。鸢尾素可促进骨形成,而抗阻运动可显著提高鸢尾素水平,并可能通过激活Wnt/β-连环蛋白信号通路等促进骨细胞增殖和分化。基质金属蛋白酶2在骨重塑过程中发挥重要作用,它能够降解骨基质成分、维持骨吸收和形成之间的平衡。抗阻运动通过调节基质金属蛋白酶2活性可能有助于维持骨组织的正常结构和功能。胰岛素样生长因子1对骨骼生长和发育具有显著促进作用。抗阻运动能够增加胰岛素样生长因子1的表达和分泌,进而促进骨细胞增殖、分化和矿化,提高骨密度和强度。抗阻运动通过调节肌肉生长抑制素、鸢尾素、基质金属蛋白酶2和胰岛素样生长因子1等肌因子的表达和活性,对骨代谢产生积极影响,有助于预防和治疗骨质疏松症,然而具体机制仍需深入研究,以进一步阐明抗阻运动对肌骨系统的整体调控作用。
