目的:比较不同的根尖孔直径以及生物陶瓷材料充填的不同厚度下,iRoot BP Plus进行根尖屏障治疗时根尖微渗漏的情况。方法:收集新鲜完整的下颌单根管前磨牙共100颗,经根管预备、建立根尖孔模型后按2个影响因素分为6个实验组(n=15)和2个...目的:比较不同的根尖孔直径以及生物陶瓷材料充填的不同厚度下,iRoot BP Plus进行根尖屏障治疗时根尖微渗漏的情况。方法:收集新鲜完整的下颌单根管前磨牙共100颗,经根管预备、建立根尖孔模型后按2个影响因素分为6个实验组(n=15)和2个对照组(n=5),选择iRoot BP Plus作为根尖屏障充填材料,实验组分为2个方向:其中A、B、C组为根尖孔直径组(0.5 mm、0.7 mm、1.0 mm),D、E、F组为根尖屏障材料充填厚度组(封闭根尖3 mm、4 mm、5 mm),G、H组分别为阳、阴性对照组。经过染料渗透实验后,比较iRoot BP Plus在不同情况下行根尖屏障术的根尖微渗漏情况。结果:在根尖孔直径组中,B组和C组的根尖微渗漏值大于A组、有统计学差异(P<0.001),但B组和C组间渗漏值无统计差异(P>0.05)。在根尖屏障材料充填厚度组中,D组、E组和F组渗漏值无统计学差异(P>0.05)。结论:iRoot BP Plus作为根尖屏障术充填材料,当患牙根尖孔直径为0.5 mm时,生物陶瓷材料充填厚度3~5 mm时根尖封闭效果并没有显著差异。在根尖孔直径达到0.7、1.0 mm条件下,根尖区会有微渗漏的发生。展开更多
Nanotechnology has provided thousands of novel nano-antimicrobials possessing features uncommon in clinically available antimicrobials.Here,nanocarriers loaded with conventional antimicrobials and responding to enviro...Nanotechnology has provided thousands of novel nano-antimicrobials possessing features uncommon in clinically available antimicrobials.Here,nanocarriers loaded with conventional antimicrobials and responding to environmental changes upon entry into oral biofilms are reviewed.Supra-gingival biofilms are characterized by acidic pH,the presence of bacterial enzymes,and the development of hypoxia in deeper layers.Sub-gingival biofilms are slightly alkaline,with hypoxia occurring over their entire depth.Upon entering biofilms,negatively charged,pH-and/or hypoxia-responsive nanocarriers become positively charged.This charge reversal leads to electrostatic double-layer attraction between positively charged nanocarriers towards negatively charged,waterfilled channel walls in biofilms,enhancing their accumulation in a biofilm.Degradation of bacterial enzyme-responsive nanocarriers causes in-biofilm release of antimicrobial cargo,yielding higher local antimicrobial concentrations than can be achieved through their direct,oral administration without harming soft tissues.Enhanced antibiofilm activity after in-biofilm antimicrobial release from biofilm-responsive micelles and liposomes has been demonstrated in vitro towards single-species Streptococcus mutans and Staphylococcus aureus biofilms or in vivo using specific-pathogen-free rodents inoculated with selected pathogens.This preferential antibacterial activity regulated the microbial composition of ex vivo human oral biofilm towards a more healthy microbiome composition.Although clinical confirmation is limited,the potential benefits of stimuli-responsive,antimicrobial-loaded nanocarriers for oral biofilm control and microbiome restoration are worth further investigation towards clinical translation.展开更多
文摘目的:比较不同的根尖孔直径以及生物陶瓷材料充填的不同厚度下,iRoot BP Plus进行根尖屏障治疗时根尖微渗漏的情况。方法:收集新鲜完整的下颌单根管前磨牙共100颗,经根管预备、建立根尖孔模型后按2个影响因素分为6个实验组(n=15)和2个对照组(n=5),选择iRoot BP Plus作为根尖屏障充填材料,实验组分为2个方向:其中A、B、C组为根尖孔直径组(0.5 mm、0.7 mm、1.0 mm),D、E、F组为根尖屏障材料充填厚度组(封闭根尖3 mm、4 mm、5 mm),G、H组分别为阳、阴性对照组。经过染料渗透实验后,比较iRoot BP Plus在不同情况下行根尖屏障术的根尖微渗漏情况。结果:在根尖孔直径组中,B组和C组的根尖微渗漏值大于A组、有统计学差异(P<0.001),但B组和C组间渗漏值无统计差异(P>0.05)。在根尖屏障材料充填厚度组中,D组、E组和F组渗漏值无统计学差异(P>0.05)。结论:iRoot BP Plus作为根尖屏障术充填材料,当患牙根尖孔直径为0.5 mm时,生物陶瓷材料充填厚度3~5 mm时根尖封闭效果并没有显著差异。在根尖孔直径达到0.7、1.0 mm条件下,根尖区会有微渗漏的发生。
基金supported by the National Science Fund for Excellent Young Scholars (32322044)the CQMU Program for Youth Innovation in Future Medicine (W0077)+1 种基金the Program for Scientific and Technological Innovation Leader of Chongqing (CQYC20220303655)the Young Scientists Fund of the National Natural Science Foundation of China (82301144)。
文摘Nanotechnology has provided thousands of novel nano-antimicrobials possessing features uncommon in clinically available antimicrobials.Here,nanocarriers loaded with conventional antimicrobials and responding to environmental changes upon entry into oral biofilms are reviewed.Supra-gingival biofilms are characterized by acidic pH,the presence of bacterial enzymes,and the development of hypoxia in deeper layers.Sub-gingival biofilms are slightly alkaline,with hypoxia occurring over their entire depth.Upon entering biofilms,negatively charged,pH-and/or hypoxia-responsive nanocarriers become positively charged.This charge reversal leads to electrostatic double-layer attraction between positively charged nanocarriers towards negatively charged,waterfilled channel walls in biofilms,enhancing their accumulation in a biofilm.Degradation of bacterial enzyme-responsive nanocarriers causes in-biofilm release of antimicrobial cargo,yielding higher local antimicrobial concentrations than can be achieved through their direct,oral administration without harming soft tissues.Enhanced antibiofilm activity after in-biofilm antimicrobial release from biofilm-responsive micelles and liposomes has been demonstrated in vitro towards single-species Streptococcus mutans and Staphylococcus aureus biofilms or in vivo using specific-pathogen-free rodents inoculated with selected pathogens.This preferential antibacterial activity regulated the microbial composition of ex vivo human oral biofilm towards a more healthy microbiome composition.Although clinical confirmation is limited,the potential benefits of stimuli-responsive,antimicrobial-loaded nanocarriers for oral biofilm control and microbiome restoration are worth further investigation towards clinical translation.
