[目的]介绍经皮椎弓钉固定联合射频消融治疗多发性骨髓瘤椎体骨折的手术技术与初步临床疗效。[方法]2020年7月—2023年7月对21例多发性骨髓瘤椎体骨折行上述治疗。跨病椎上下分别各置入1组经皮椎弓根螺钉,于伤椎主要病变侧穿刺。置入套...[目的]介绍经皮椎弓钉固定联合射频消融治疗多发性骨髓瘤椎体骨折的手术技术与初步临床疗效。[方法]2020年7月—2023年7月对21例多发性骨髓瘤椎体骨折行上述治疗。跨病椎上下分别各置入1组经皮椎弓根螺钉,于伤椎主要病变侧穿刺。置入套管,取活组织检查后,置入射频电极,并于同侧椎弓根处放置测温针行术中温度监测,必要时行术中脊髓神经电生理监测,启动热消融,给予2~3个周期的热消融处理。置入预弯双侧棒,调整紧固钉-棒系统,缝合切口。术后佩戴支具进行康复功能锻炼,并进一步接受系统内科治疗。[结果]21例患者均顺利完成手术,无血管、神经损伤等严重并发症。本组患者随访18个月以上,与术前相比,末次随访时疼痛评分(visual analog scale,VAS)[分,(7.6±0.8),(2.0±0.8),P<0.001]显著下降,而KPS评分(Karnofsky Performance Status)[分,(51.4±1.8),(90.0±1.3),P<0.001]和Frankal评分[分,(3.4±1.8),(5.0±0.3),P<0.001]显著增加。局部脊柱Cobb角术后矫正率>80%,无内固定松动或断裂。[结论]经皮椎弓钉固定联合射频消融术不仅微创,而且在缓解疼痛、稳定椎体、控制肿瘤进展等方面疗效确切。展开更多
1概述骨巨细胞瘤(giant cell tumor of bone,GCTB)是一种原发于骨骼的交界性肿瘤,主要由多核巨细胞和单核细胞组成。该疾病常发生在20到40岁的成年人,尤其是女性。骨巨细胞瘤多数位于长骨骨端。在临床上,该疾病具有局部侵袭性,可局部复...1概述骨巨细胞瘤(giant cell tumor of bone,GCTB)是一种原发于骨骼的交界性肿瘤,主要由多核巨细胞和单核细胞组成。该疾病常发生在20到40岁的成年人,尤其是女性。骨巨细胞瘤多数位于长骨骨端。在临床上,该疾病具有局部侵袭性,可局部复发和远处转移。展开更多
The published article titled“MicroRNA-148a Acts as a Tumor Suppressor in Osteosarcoma via Targeting Rho-Associated Coiled-Coil Kinase”has been retracted from Oncology Research,Vol.25,No.8,2017,pp.1231–1243.DOI:10.3...The published article titled“MicroRNA-148a Acts as a Tumor Suppressor in Osteosarcoma via Targeting Rho-Associated Coiled-Coil Kinase”has been retracted from Oncology Research,Vol.25,No.8,2017,pp.1231–1243.DOI:10.3727/096504017X14850134190255 URL:https://www.techscience.com/or/v25n8/56908 Following the publication,concerns have been raised about a number of figures in this article.An unexpected area of similarity was identified in terms of the cellular data,where the results from differently performed experiments were intended to have been shown,although the areas immediately surrounding this area featured comparatively different distributions of cells.In addition,the western blots in this article were presented with atypical,unusually shaped and possibly anomalous protein bands in many cases.展开更多
Background:Photodynamic therapy(PDT)may eradicate residual malignant cells following sarcoma resection,through reactive oxygen species(ROS)mediated cytotoxicity,thus improve clinical outcomes.This study aims to assess...Background:Photodynamic therapy(PDT)may eradicate residual malignant cells following sarcoma resection,through reactive oxygen species(ROS)mediated cytotoxicity,thus improve clinical outcomes.This study aims to assess the efficacy of 5-aminolevulinic acid(5-ALA)as a photosensitizer in combination with red light(RL)for PDT of bone sarcoma cells in vitro.Methods:Three bone sarcoma cell lines underwent treatment with 5-ALA and RL or sham-RL(SL).5-ALA uptake was assessed using flow cytometry.Production of ROS was measured using CellROX Green staining and fluorescence microscopy.Cell viability was assessed using Cell Counting Kit-8 assays.Results:All cell lines showed significant 5-ALA uptake in comparison to the 0 mM control(p<0.05).Production of ROS was significantly increased in cells treated with 5-ALA and RL,compared to those treated with RL and no 5-ALA or SL(p<0.05).Viability was significantly reduced in cells treated with 5-ALA and RL,compared to SL(p<0.05).At 72 h post-treatment,cell viability ranged from 6%-12%in 0.5 mM 5-ALA and RL-treated cells vs.90%-137%in 0.5 mM 5-ALA and SL-treated cells.Conclusion:5-ALA-based PDT led to the desired increased production of ROS and reduction in cell viability in all cell lines.These preliminary in vitro results warrant further study with multicellular spheroid or animal models and suggest PDT has potential to be used as an adjuvant therapy to surgical resection in sarcoma management.展开更多
文摘[目的]介绍经皮椎弓钉固定联合射频消融治疗多发性骨髓瘤椎体骨折的手术技术与初步临床疗效。[方法]2020年7月—2023年7月对21例多发性骨髓瘤椎体骨折行上述治疗。跨病椎上下分别各置入1组经皮椎弓根螺钉,于伤椎主要病变侧穿刺。置入套管,取活组织检查后,置入射频电极,并于同侧椎弓根处放置测温针行术中温度监测,必要时行术中脊髓神经电生理监测,启动热消融,给予2~3个周期的热消融处理。置入预弯双侧棒,调整紧固钉-棒系统,缝合切口。术后佩戴支具进行康复功能锻炼,并进一步接受系统内科治疗。[结果]21例患者均顺利完成手术,无血管、神经损伤等严重并发症。本组患者随访18个月以上,与术前相比,末次随访时疼痛评分(visual analog scale,VAS)[分,(7.6±0.8),(2.0±0.8),P<0.001]显著下降,而KPS评分(Karnofsky Performance Status)[分,(51.4±1.8),(90.0±1.3),P<0.001]和Frankal评分[分,(3.4±1.8),(5.0±0.3),P<0.001]显著增加。局部脊柱Cobb角术后矫正率>80%,无内固定松动或断裂。[结论]经皮椎弓钉固定联合射频消融术不仅微创,而且在缓解疼痛、稳定椎体、控制肿瘤进展等方面疗效确切。
文摘The published article titled“MicroRNA-148a Acts as a Tumor Suppressor in Osteosarcoma via Targeting Rho-Associated Coiled-Coil Kinase”has been retracted from Oncology Research,Vol.25,No.8,2017,pp.1231–1243.DOI:10.3727/096504017X14850134190255 URL:https://www.techscience.com/or/v25n8/56908 Following the publication,concerns have been raised about a number of figures in this article.An unexpected area of similarity was identified in terms of the cellular data,where the results from differently performed experiments were intended to have been shown,although the areas immediately surrounding this area featured comparatively different distributions of cells.In addition,the western blots in this article were presented with atypical,unusually shaped and possibly anomalous protein bands in many cases.
文摘Background:Photodynamic therapy(PDT)may eradicate residual malignant cells following sarcoma resection,through reactive oxygen species(ROS)mediated cytotoxicity,thus improve clinical outcomes.This study aims to assess the efficacy of 5-aminolevulinic acid(5-ALA)as a photosensitizer in combination with red light(RL)for PDT of bone sarcoma cells in vitro.Methods:Three bone sarcoma cell lines underwent treatment with 5-ALA and RL or sham-RL(SL).5-ALA uptake was assessed using flow cytometry.Production of ROS was measured using CellROX Green staining and fluorescence microscopy.Cell viability was assessed using Cell Counting Kit-8 assays.Results:All cell lines showed significant 5-ALA uptake in comparison to the 0 mM control(p<0.05).Production of ROS was significantly increased in cells treated with 5-ALA and RL,compared to those treated with RL and no 5-ALA or SL(p<0.05).Viability was significantly reduced in cells treated with 5-ALA and RL,compared to SL(p<0.05).At 72 h post-treatment,cell viability ranged from 6%-12%in 0.5 mM 5-ALA and RL-treated cells vs.90%-137%in 0.5 mM 5-ALA and SL-treated cells.Conclusion:5-ALA-based PDT led to the desired increased production of ROS and reduction in cell viability in all cell lines.These preliminary in vitro results warrant further study with multicellular spheroid or animal models and suggest PDT has potential to be used as an adjuvant therapy to surgical resection in sarcoma management.
