Peripheral nerve injury causes severe neuroinflammation and has become a global medical challenge.Previous research has demonstrated that porcine decellularized nerve matrix hydrogel exhibits excellent biological prop...Peripheral nerve injury causes severe neuroinflammation and has become a global medical challenge.Previous research has demonstrated that porcine decellularized nerve matrix hydrogel exhibits excellent biological properties and tissue specificity,highlighting its potential as a biomedical material for the repair of severe peripheral nerve injury;however,its role in modulating neuroinflammation post-peripheral nerve injury remains unknown.Here,we aimed to characterize the anti-inflammatory properties of porcine decellularized nerve matrix hydrogel and their underlying molecular mechanisms.Using peripheral nerve injury model rats treated with porcine decellularized nerve matrix hydrogel,we evaluated structural and functional recovery,macrophage phenotype alteration,specific cytokine expression,and changes in related signaling molecules in vivo.Similar parameters were evaluated in vitro using monocyte/macrophage cell lines stimulated with lipopolysaccharide and cultured on porcine decellularized nerve matrix hydrogel-coated plates in complete medium.These comprehensive analyses revealed that porcine decellularized nerve matrix hydrogel attenuated the activation of excessive inflammation at the early stage of peripheral nerve injury and increased the proportion of the M2 subtype in monocytes/macrophages.Additionally,porcine decellularized nerve matrix hydrogel negatively regulated the Toll-like receptor 4/myeloid differentiation factor 88/nuclear factor-κB axis both in vivo and in vitro.Our findings suggest that the efficacious anti-inflammatory properties of porcine decellularized nerve matrix hydrogel induce M2 macrophage polarization via suppression of the Toll-like receptor 4/myeloid differentiation factor 88/nuclear factor-κB pathway,providing new insights into the therapeutic mechanism of porcine decellularized nerve matrix hydrogel in peripheral nerve injury.展开更多
目的分析面肌痉挛患者行显微血管减压术的效果及术后听力障碍的影响因素。方法前瞻性选取2021年1月至2023年5月西安交通大学附属红会医院收治的108例面肌痉挛患者作为研究对象。按照随机数字表法将其分为对照组和观察组,每组各54例。两...目的分析面肌痉挛患者行显微血管减压术的效果及术后听力障碍的影响因素。方法前瞻性选取2021年1月至2023年5月西安交通大学附属红会医院收治的108例面肌痉挛患者作为研究对象。按照随机数字表法将其分为对照组和观察组,每组各54例。两组患者均行显微血管减压术,其中对照组采取传统竖切口,观察组采取微创直切口。比较两组手术预后和手术指标(术中出血量、手术时间、术后瘢痕长度),手术前后的疼痛情况[视觉模拟评分法(VAS)评分]、并发症发生情况。随访12个月,根据患者术后是否发生听力障碍,分为听力障碍组(n=14)和非听力障碍组(n=94)。收集两组患者临床资料,使用单因素分析和多因素Logistic回归分析对面肌痉挛患者行显微血管减压术后听力障碍的独立危险因素进行分析。结果两组患者均顺利完成手术,即刻治愈78例,延迟治愈30例。经随访12个月,均未见复发病例。观察组的手术时间、术后瘢痕长度分别为(62.14±3.51)min、(31.26±4.08)mL,均短于对照组[(76.53±5.12)min、(56.82±6.74)mL],术中出血量为(4.20±0.87)cm,少于对照组[(6.91±1.23)cm],差异均有统计学意义(P<0.05)。术后3 d,两组VAS评分均较术前明显降低,且观察组术后3 d VAS评分为(1.16±0.15)分,低于对照组[(2.23±0.28)分],差异有统计学意义(P<0.05)。两组均未见严重并发症发生,其中观察组并发症发生率为7.41%,低于对照组(20.37%),差异有统计学意义(P<0.05)。经单因素分析和多因素Logistic回归分析,合并高血压、责任血管为椎动脉、术中听神经物理性损伤、术中听神经滋养血管痉挛均是术后听力障碍的危险因素(OR=2.636,95%CI:1.025~7.552;OR=0.528,95%CI:0.013~5.021;OR=5.452,95%CI:1.245~24.578;OR=9.234,95%CI:1.206~68.763;P<0.05)。结论面肌痉挛患者行显微血管减压术效果较好,经微创直切口可进一步减轻手术创伤,而合并高血压、责任血管为椎动脉、术中听神经物理性损伤并滋养血管痉挛均是术后听力障碍的独立危险因素。展开更多
基金supported by the Shenzhen Hong Kong Joint Funding Project,No.SGDX20230116093645007(to LY)the Shenzhen Science and Technology Innovation Committee International Cooperation Project,No.GJHZ20200731095608025(to LY)+7 种基金Shenzhen Development and Reform Commission’s Intelligent Diagnosis,Treatment and Prevention of Adolescent Spinal Health Public Service Platform,No.S2002Q84500835(to LY)Shenzhen Medical Research Fund,No.B2303005(to LY)Team-based Medical Science Research Program,No.2024YZZ02(to LY)Zhejiang Provincial Natural Science Foundation of China,No.LWQ20H170001(to RL)Basic Research Project of Shenzhen Science and Technology from Shenzhen Science and Technology Innovation Commission,No.JCYJ20210324103010029(to BY)Shenzhen Second People’s Hospital Clinical Research Fund of Guangdong Province High-level Hospital Construction Project,Nos.2023yjlcyj029(to BY),2023yjlcyj021(to LL)Guangdong Basic and Applied Basic Research Foundation,No.2022A1515110679(to LL)China Postdoctoral Science Foundation,No.2022M722203(to GL).
文摘Peripheral nerve injury causes severe neuroinflammation and has become a global medical challenge.Previous research has demonstrated that porcine decellularized nerve matrix hydrogel exhibits excellent biological properties and tissue specificity,highlighting its potential as a biomedical material for the repair of severe peripheral nerve injury;however,its role in modulating neuroinflammation post-peripheral nerve injury remains unknown.Here,we aimed to characterize the anti-inflammatory properties of porcine decellularized nerve matrix hydrogel and their underlying molecular mechanisms.Using peripheral nerve injury model rats treated with porcine decellularized nerve matrix hydrogel,we evaluated structural and functional recovery,macrophage phenotype alteration,specific cytokine expression,and changes in related signaling molecules in vivo.Similar parameters were evaluated in vitro using monocyte/macrophage cell lines stimulated with lipopolysaccharide and cultured on porcine decellularized nerve matrix hydrogel-coated plates in complete medium.These comprehensive analyses revealed that porcine decellularized nerve matrix hydrogel attenuated the activation of excessive inflammation at the early stage of peripheral nerve injury and increased the proportion of the M2 subtype in monocytes/macrophages.Additionally,porcine decellularized nerve matrix hydrogel negatively regulated the Toll-like receptor 4/myeloid differentiation factor 88/nuclear factor-κB axis both in vivo and in vitro.Our findings suggest that the efficacious anti-inflammatory properties of porcine decellularized nerve matrix hydrogel induce M2 macrophage polarization via suppression of the Toll-like receptor 4/myeloid differentiation factor 88/nuclear factor-κB pathway,providing new insights into the therapeutic mechanism of porcine decellularized nerve matrix hydrogel in peripheral nerve injury.
文摘目的分析面肌痉挛患者行显微血管减压术的效果及术后听力障碍的影响因素。方法前瞻性选取2021年1月至2023年5月西安交通大学附属红会医院收治的108例面肌痉挛患者作为研究对象。按照随机数字表法将其分为对照组和观察组,每组各54例。两组患者均行显微血管减压术,其中对照组采取传统竖切口,观察组采取微创直切口。比较两组手术预后和手术指标(术中出血量、手术时间、术后瘢痕长度),手术前后的疼痛情况[视觉模拟评分法(VAS)评分]、并发症发生情况。随访12个月,根据患者术后是否发生听力障碍,分为听力障碍组(n=14)和非听力障碍组(n=94)。收集两组患者临床资料,使用单因素分析和多因素Logistic回归分析对面肌痉挛患者行显微血管减压术后听力障碍的独立危险因素进行分析。结果两组患者均顺利完成手术,即刻治愈78例,延迟治愈30例。经随访12个月,均未见复发病例。观察组的手术时间、术后瘢痕长度分别为(62.14±3.51)min、(31.26±4.08)mL,均短于对照组[(76.53±5.12)min、(56.82±6.74)mL],术中出血量为(4.20±0.87)cm,少于对照组[(6.91±1.23)cm],差异均有统计学意义(P<0.05)。术后3 d,两组VAS评分均较术前明显降低,且观察组术后3 d VAS评分为(1.16±0.15)分,低于对照组[(2.23±0.28)分],差异有统计学意义(P<0.05)。两组均未见严重并发症发生,其中观察组并发症发生率为7.41%,低于对照组(20.37%),差异有统计学意义(P<0.05)。经单因素分析和多因素Logistic回归分析,合并高血压、责任血管为椎动脉、术中听神经物理性损伤、术中听神经滋养血管痉挛均是术后听力障碍的危险因素(OR=2.636,95%CI:1.025~7.552;OR=0.528,95%CI:0.013~5.021;OR=5.452,95%CI:1.245~24.578;OR=9.234,95%CI:1.206~68.763;P<0.05)。结论面肌痉挛患者行显微血管减压术效果较好,经微创直切口可进一步减轻手术创伤,而合并高血压、责任血管为椎动脉、术中听神经物理性损伤并滋养血管痉挛均是术后听力障碍的独立危险因素。
